JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sharma, P. M.
Right arrow Articles by Olefsky, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sharma, P. M.
Right arrow Articles by Olefsky, J. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 29, 18528-18537, July 17, 1998

Inhibition of Phosphatidylinositol 3-Kinase Activity by Adenovirus-mediated Gene Transfer and Its Effect on Insulin Action

Prem M. SharmaDagger , Katsuya EgawaDagger , Yi HuangDagger , Jody L. MartinDagger , Ivana Huvar§, Gerry R. Boss§, and Jerrold M. OlefskyDagger parallel

From the Dagger  Department of Medicine, Division of Endocrinology and Metabolism, the parallel  Whittier Diabetes Institute, and the § Department of Medicine, University of California, San Diego, La Jolla, California 92093, the  Veterans Administration Research Service, San Diego, California 92161

Phosphatidylinositol 3-kinase (PI 3-K) is implicated in cellular events including glucose transport, glycogen synthesis, and protein synthesis. It is activated in insulin-stimulated cells by binding of the Src homology 2 (SH2) domains in its 85-kDa regulatory subunit to insulin receptor substrate-1 (IRS-1), and, others. We have previously shown that IRS-1-associated PI 3-kinase activity is not essential for insulin-stimulated glucose transport in 3T3-L1 adipocytes, and that alternate pathways exist in these cells. We now show that adenovirus-mediated overexpression of the p85N-SH2 domain in these cells behaves in a dominant-negative manner, interfering with complex formation between endogenous PI 3-K and its SH2 binding targets. This not only inhibited insulin-stimulated IRS-1-associated PI 3-kinase activity, but also completely blocked anti-phosphotyrosine-associated PI 3-kinase activity, which would include the non-IRS-1-associated activity. This resulted in inhibition of insulin-stimulated glucose transport, glycogen synthase activity and DNA synthesis. Further, Ser/Thr phosphorylation of downstream molecules Akt and p70 S6 kinase was inhibited. However, co-expression of a membrane-targeted p110C with the p85N-SH2 protein rescued glucose transport, supporting our argument that the p85N-SH2 protein specifically blocks insulin-mediated PI 3-kinase activity, and, that the signaling pathways downstream of PI 3-kinase are intact. Unexpectedly, GTP-bound Ras was elevated in the basal state. Since p85 is known to interact with GTPase-activating protein in 3T3-L1 adipocytes, the overexpressed p85N-SH2 peptide could titrate out cellular GTPase-activating protein by direct association, such that it is unavailable to hydrolyze GTP-bound Ras. However, insulin-induced mitogen-activated protein kinase phosphorylation was inhibited. Thus, PI 3-kinase may be required for this action at a step independent of and downstream of Ras. We conclude that, in 3T3-L1 adipocytes, non-IRS-1-associated PI 3-kinase activity is crucial for insulin's metabolic signaling, and that overexpressed p85N-SH2 protein inhibits a variety of insulin's ultimate biological effects.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
F. Hakuno, S. Kurihara, R. T. Watson, J. E. Pessin, and S.-I. Takahashi
53BP2S, Interacting with Insulin Receptor Substrates, Modulates Insulin Signaling
J. Biol. Chem., December 28, 2007; 282(52): 37747 - 37758.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Y. Jiang, Q. L. Zhou, J. Holik, S. Patel, J. Leszyk, K. Coleman, M. Chouinard, and M. P. Czech
Identification of WNK1 as a Substrate of Akt/Protein Kinase B and a Negative Regulator of Insulin-stimulated Mitogenesis in 3T3-L1 Cells
J. Biol. Chem., June 3, 2005; 280(22): 21622 - 21628.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Nishi, E. D. Werner, B.-C. Oh, J. D. Frantz, S. Dhe-Paganon, L. Hansen, J. Lee, and S. E. Shoelson
Kinase Activation through Dimerization by Human SH2-B
Mol. Cell. Biol., April 1, 2005; 25(7): 2607 - 2621.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
P. M. Sharma, H.-S. Son, S. Ugi, W. Ricketts, and J. M. Olefsky
Mechanism of SHIP-Mediated Inhibition of Insulin- and Platelet-Derived Growth Factor-Stimulated Mitogen-Activated Protein Kinase Activity in 3T3-L1 Adipocytes
Mol. Endocrinol., February 1, 2005; 19(2): 421 - 430.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Mitra, X. Zheng, and M. P. Czech
RNAi-based Analysis of CAP, Cbl, and CrkII Function in the Regulation of GLUT4 by Insulin
J. Biol. Chem., September 3, 2004; 279(36): 37431 - 37435.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Duan, H. Yang, M. F. White, and L. Rui
Disruption of the SH2-B Gene Causes Age-Dependent Insulin Resistance and Glucose Intolerance
Mol. Cell. Biol., September 1, 2004; 24(17): 7435 - 7443.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
N. Hay and N. Sonenberg
Upstream and downstream of mTOR
Genes & Dev., August 15, 2004; 18(16): 1926 - 1945.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. James, C. Salaun, F. M. Brandie, J. M. C. Connell, and L. H. Chamberlain
Neomycin Prevents the Wortmannin Inhibition of Insulin-stimulated Glut4 Translocation and Glucose Transport in 3T3-L1 Adipocytes
J. Biol. Chem., May 14, 2004; 279(20): 20567 - 20570.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
R. T. Watson, M. Kanzaki, and J. E. Pessin
Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes
Endocr. Rev., April 1, 2004; 25(2): 177 - 204.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
T. E. Harris and J. C. Lawrence Jr.
TOR Signaling
Sci. Signal., December 9, 2003; 2003(212): re15 - re15.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Deng, S. Bhattacharya, O. R. Swamy, R. Tandon, Y. Wang, R. Janda, and H. Riedel
Growth Factor Receptor-binding Protein 10 (Grb10) as a Partner of Phosphatidylinositol 3-Kinase in Metabolic Insulin Action
J. Biol. Chem., October 10, 2003; 278(41): 39311 - 39322.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Chunqiu Hou and J. E. Pessin
Lipid Raft Targeting of the TC10 Amino Terminal Domain Is Responsible for Disruption of Adipocyte Cortical Actin
Mol. Biol. Cell, September 1, 2003; 14(9): 3578 - 3591.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. Tardif, N. Julien, J.-L. Chiasson, and L. Coderre
Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation
Am J Physiol Endocrinol Metab, June 1, 2003; 284(6): E1055 - E1064.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Ijuin and T. Takenawa
SKIP Negatively Regulates Insulin-Induced GLUT4 Translocation and Membrane Ruffle Formation
Mol. Cell. Biol., February 15, 2003; 23(4): 1209 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. C. Chen, S. Zhuang, T. H. Nguyen, G. R. Boss, and R. B. Pilz
Oncogenic Ras Leads to Rho Activation by Activating the Mitogen-activated Protein Kinase Pathway and Decreasing Rho-GTPase-activating Protein Activity
J. Biol. Chem., January 24, 2003; 278(5): 2807 - 2818.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Farhang-Fallah, V. K. Randhawa, A. Nimnual, A. Klip, D. Bar-Sagi, and M. Rozakis-Adcock
The Pleckstrin Homology (PH) Domain-Interacting Protein Couples the Insulin Receptor Substrate 1 PH Domain to Insulin Signaling Pathways Leading to Mitogenesis and GLUT4 Translocation
Mol. Cell. Biol., October 15, 2002; 22(20): 7325 - 7336.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Gudi, J. C. Chen, D. E. Casteel, T. M. Seasholtz, G. R. Boss, and R. B. Pilz
cGMP-dependent Protein Kinase Inhibits Serum-response Element-dependent Transcription by Inhibiting Rho Activation and Functions
J. Biol. Chem., September 27, 2002; 277(40): 37382 - 37393.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ugi, P. M. Sharma, W. Ricketts, T. Imamura, and J. M. Olefsky
Phosphatidylinositol 3-Kinase Is Required for Insulin-stimulated Tyrosine Phosphorylation of Shc in 3T3-L1 Adipocytes
J. Biol. Chem., May 17, 2002; 277(21): 18592 - 18597.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Y. Jiang, A. Chawla, A. Bose, M. Way, and M. P. Czech
A Phosphatidylinositol 3-Kinase-independent Insulin Signaling Pathway to N-WASP/Arp2/3/F-actin Required for GLUT4 Glucose Transporter Recycling
J. Biol. Chem., January 4, 2002; 277(1): 509 - 515.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. G. Park, A. Bose, J. Leszyk, and M. P. Czech
PYK2 as a Mediator of Endothelin-1/Galpha 11 Signaling to GLUT4 Glucose Transporters
J. Biol. Chem., December 14, 2001; 276(51): 47751 - 47754.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Bose, A. D. Cherniack, S. E. Langille, S. M. C. Nicoloro, J. M. Buxton, J. G. Park, A. Chawla, and M. P. Czech
G{alpha}11 Signaling through ARF6 Regulates F-Actin Mobilization and GLUT4 Glucose Transporter Translocation to the Plasma Membrane
Mol. Cell. Biol., August 1, 2001; 21(15): 5262 - 5275.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Y. Tu, A. Gardner, and A. Lichtenstein
The Phosphatidylinositol 3-Kinase/AKT Kinase Pathway in Multiple Myeloma Plasma Cells: Roles in Cytokine-dependent Survival and Proliferative Responses
Cancer Res., December 1, 2000; 60(23): 6763 - 6770.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
K. Ueki, P. Algenstaedt, F. Mauvais-Jarvis, and C. R. Kahn
Positive and Negative Regulation of Phosphoinositide 3-Kinase-Dependent Signaling Pathways by Three Different Gene Products of the p85alpha Regulatory Subunit
Mol. Cell. Biol., November 1, 2000; 20(21): 8035 - 8046.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
K. M. Kriauciunas, M. G. Myers Jr., and C. R. Kahn
Cellular Compartmentalization in Insulin Action: Altered Signaling by a Lipid-Modified IRS-1
Mol. Cell. Biol., September 15, 2000; 20(18): 6849 - 6859.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
G. Razzini, A. Ingrosso, A. Brancaccio, S. Sciacchitano, D. L. Esposito, and M. Falasca
Different Subcellular Localization and Phosphoinositides Binding of Insulin Receptor Substrate Protein Pleckstrin Homology Domains
Mol. Endocrinol., June 1, 2000; 14(6): 823 - 836.
[Abstract] [Full Text]

Home page
 FASEB J.Home page
J. LI, K. L. HOUSEKNECHT, A. E. STENBIT, E. B. KATZ, and M. J. CHARRON
Reduced glucose uptake precedes insulin signaling defects in adipocytes from heterozygous GLUT4 knockout mice
FASEB J, June 1, 2000; 14(9): 1117 - 1125.
[Abstract] [Full Text]

Home page
 Clin. Cancer Res.Home page
F. C. von Lintig, I. Huvar, P. Law, M. B. Diccianni, A. L. Yu, and G. R. Boss
Ras Activation in Normal White Blood Cells and Childhood Acute Lymphoblastic Leukemia
Clin. Cancer Res., May 1, 2000; 6(5): 1804 - 1810.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. Nakashima, P. M. Sharma, T. Imamura, R. Bookstein, and J. M. Olefsky
The Tumor Suppressor PTEN Negatively Regulates Insulin Signaling in 3T3-L1 Adipocytes
J. Biol. Chem., April 21, 2000; 275(17): 12889 - 12895.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Tong, W. Chen, R. E. London, E. Murphy, and C. Steenbergen
Preconditioning Enhanced Glucose Uptake Is Mediated by p38 MAP Kinase Not by Phosphatidylinositol 3-Kinase
J. Biol. Chem., April 14, 2000; 275(16): 11981 - 11986.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Kainulainen, M. Sundvall, J. A. Maatta, E. Santiestevan, M. Klagsbrun, and K. Elenius
A Natural ErbB4 Isoform That Does Not Activate Phosphoinositide 3-Kinase Mediates Proliferation but Not Survival or Chemotaxis
J. Biol. Chem., March 17, 2000; 275(12): 8641 - 8649.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Emoto, J. K. Klarlund, S. B. Waters, V. Hu, J. M. Buxton, A. Chawla, and M. P. Czech
A Role for Phospholipase D in GLUT4 Glucose Transporter Translocation
J. Biol. Chem., March 15, 2000; 275(10): 7144 - 7151.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Tian, G. R. Boss, and D. M. Cohen
Ras signaling in the inner medullary cell response to urea and NaCl
Am J Physiol Cell Physiol, February 1, 2000; 278(2): C372 - C380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Eguchi, H. Iwasaki, H. Ueno, G. D. Frank, E. D. Motley, K. Eguchi, F. Marumo, Y. Hirata, and T. Inagami
Intracellular Signaling of Angiotensin II-induced p70 S6 Kinase Phosphorylation at Ser411 in Vascular Smooth Muscle Cells. POSSIBLE REQUIREMENT OF EPIDERMAL GROWTH FACTOR RECEPTOR, RAS, EXTRACELLULAR SIGNAL-REGULATED KINASE, AND AKT
J. Biol. Chem., December 24, 1999; 274(52): 36843 - 36851.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Imamura, K.-i. Ishibashi, S. Dalle, S. Ugi, and J. M. Olefsky
Endothelin-1-induced GLUT4 Translocation Is Mediated via Galpha q/11 Protein and Phosphatidylinositol 3-Kinase in 3T3-L1 Adipocytes
J. Biol. Chem., November 19, 1999; 274(47): 33691 - 33695.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. M. Hill, S. F. Clark, D. F. Tucker, M. J. Birnbaum, D. E. James, and S. L. Macaulay
A Role for Protein Kinase Bbeta /Akt2 in Insulin-Stimulated GLUT4 Translocation in Adipocytes
Mol. Cell. Biol., November 1, 1999; 19(11): 7771 - 7781.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Larsson, P. Klint, E. Landgren, and L. Claesson-Welsh
Fibroblast Growth Factor Receptor-1-mediated Endothelial Cell Proliferation Is Dependent on the Src Homology (SH) 2/SH3 Domain-containing Adaptor Protein Crk
J. Biol. Chem., September 3, 1999; 274(36): 25726 - 25734.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. F. Hausdorff, D. C. Fingar, K. Morioka, L. A. Garza, E. L. Whiteman, S. A. Summers, and M. J. Birnbaum
Identification of Wortmannin-sensitive Targets in 3T3-L1 Adipocytes. DISSOCIATION OF INSULIN-STIMULATED GLUCOSE UPTAKE AND GLUT4 TRANSLOCATION
J. Biol. Chem., August 27, 1999; 274(35): 24677 - 24684.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Summers, E. L. Whiteman, H. Cho, L. Lipfert, and M. J. Birnbaum
Differentiation-dependent Suppression of Platelet-derived Growth Factor Signaling in Cultured Adipocytes
J. Biol. Chem., August 20, 1999; 274(34): 23858 - 23867.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Takata, W. Ogawa, T. Kitamura, Y. Hino, S. Kuroda, K. Kotani, A. Klip, A.-C. Gingras, N. Sonenberg, and M. Kasuga
Requirement for Akt (Protein Kinase B) in Insulin-induced Activation of Glycogen Synthase and Phosphorylation of 4E-BP1 (PHAS-1)
J. Biol. Chem., July 16, 1999; 274(29): 20611 - 20618.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Egawa, P. M. Sharma, N. Nakashima, Y. Huang, E. Huver, G. R. Boss, and J. M. Olefsky
Membrane-targeted Phosphatidylinositol 3-Kinase Mimics Insulin Actions and Induces a State of Cellular Insulin Resistance
J. Biol. Chem., May 14, 1999; 274(20): 14306 - 14314.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Anrather, V. Csizmadia, M. P. Soares, and H. Winkler
Regulation of NF-kappa B RelA Phosphorylation and Transcriptional Activity by p21ras and Protein Kinase Czeta in Primary Endothelial Cells
J. Biol. Chem., May 7, 1999; 274(19): 13594 - 13603.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Bi, I. Okabe, D. J. Bernard, A. Wynshaw-Boris, and R. L. Nussbaum
Proliferative Defect and Embryonic Lethality in Mice Homozygous for a Deletion in the p110alpha Subunit of Phosphoinositide 3-Kinase
J. Biol. Chem., April 16, 1999; 274(16): 10963 - 10968.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Moodie, J. Alleman-Sposeto, and T. A. Gustafson
Identification of the APS Protein as a Novel Insulin Receptor Substrate
J. Biol. Chem., April 16, 1999; 274(16): 11186 - 11193.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. L. Craddock, E. A. Orchiston, H. J. Hinton, and M. J. Welham
Dissociation of Apoptosis from Proliferation, Protein Kinase B Activation, and BAD Phosphorylation in Interleukin-3-mediated Phosphoinositide 3-Kinase Signaling
J. Biol. Chem., April 9, 1999; 274(15): 10633 - 10640.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. P. Czech and S. Corvera
Signaling Mechanisms That Regulate Glucose Transport
J. Biol. Chem., January 22, 1999; 274(4): 1865 - 1868.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Yenush, C. Zanella, T. Uchida, D. Bernal, and M. F. White
The Pleckstrin Homology and Phosphotyrosine Binding Domains of Insulin Receptor Substrate 1 Mediate Inhibition of Apoptosis by Insulin
Mol. Cell. Biol., November 1, 1998; 18(11): 6784 - 6794.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. M. Hill, L. M. Connolly, R. J. Simpson, and D. E. James
Differential Protein Phosphorylation in 3T3-L1 Adipocytes in Response to Insulin Versus Platelet-derived Growth Factor. NO EVIDENCE FOR A PHOSPHATIDYLINOSITIDE 3-KINASE-INDEPENDENT PATHWAY IN INSULIN SIGNALING
J. Biol. Chem., August 4, 2000; 275(32): 24313 - 24320.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Qiu, S. Zhuang, F. C. von Lintig, G. R. Boss, and R. B. Pilz
Cell Type-specific Regulation of B-Raf Kinase by cAMP and 14-3-3 Proteins
J. Biol. Chem., October 6, 2000; 275(41): 31921 - 31929.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Fasshauer, J. Klein, K. Ueki, K. M. Kriauciunas, M. Benito, M. F. White, and C. R. Kahn
Essential Role of Insulin Receptor Substrate-2 in Insulin Stimulation of Glut4 Translocation and Glucose Uptake in Brown Adipocytes
J. Biol. Chem., August 11, 2000; 275(33): 25494 - 25501.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Dupuy, K. Morgan, F. C. von Lintig, H. Shen, H. Acar, D. E. Hasz, N. A. Jenkins, N. G. Copeland, G. R. Boss, and D. A. Largaespada
Activation of the Rap1 Guanine Nucleotide Exchange Gene, CalDAG-GEF I, in BXH-2 Murine Myeloid Leukemia
J. Biol. Chem., April 6, 2001; 276(15): 11804 - 11811.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. L. Craddock, J. Hobbs, C. E. Edmead, and M. J. Welham
Phosphoinositide 3-Kinase-dependent Regulation of Interleukin-3-induced Proliferation. INVOLVEMENT OF MITOGEN-ACTIVATED PROTEIN KINASES, SHP2 AND Gab2
J. Biol. Chem., June 22, 2001; 276(26): 24274 - 24283.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. L. Goalstone, J. W. Leitner, P. Berhanu, P. M. Sharma, J. M. Olefsky, and B. Draznin
Insulin Signals to Prenyltransferases via the Shc Branch of Intracellular Signaling
J. Biol. Chem., April 13, 2001; 276(16): 12805 - 12812.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Shigematsu, S. L. Miller, and J. E. Pessin
Differentiated 3T3L1 Adipocytes Are Composed of Heterogenous Cell Populations with Distinct Receptor Tyrosine Kinase Signaling Properties
J. Biol. Chem., April 27, 2001; 276(18): 15292 - 15297.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Emoto, S. E. Langille, and M. P. Czech
A Role for Kinesin in Insulin-stimulated GLUT4 Glucose Transporter Translocation in 3T3-L1 Adipocytes
J. Biol. Chem., March 30, 2001; 276(14): 10677 - 10682.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Lee-Kwon, D. C. Johns, B. Cha, M. Cavet, J. Park, P. Tsichlis, and M. Donowitz
Constitutively Active Phosphatidylinositol 3-Kinase and AKT Are Sufficient to Stimulate the Epithelial Na+/H+ Exchanger 3
J. Biol. Chem., August 10, 2001; 276(33): 31296 - 31304.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Tremblay and A. Marette
Amino Acid and Insulin Signaling via the mTOR/p70 S6 Kinase Pathway. A NEGATIVE FEEDBACK MECHANISM LEADING TO INSULIN RESISTANCE IN SKELETAL M