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J Biol Chem, Vol. 273, Issue 29, 18586-18593, July 17, 1998
From the The polysaccharide intercellular adhesin (PIA) is
an important factor in the colonization of medical devices by
Staphylococcus epidermidis. The genes encoding PIA
production are organized in the icaADBC
(intercellular adhesion) operon. To
study the function of the individual genes, we have established an
in vitro assay with UDP-N-acetylglucosamine,
the substrate for PIA biosynthesis, and analyzed the products by
thin-layer chromatography and mass spectrometry. IcaA alone exhibited a
low N-acetylglucosaminyltransferase activity and represents
the catalytic enzyme. Coexpression of icaA with
icaD led to a significant increase in activity. The newly
identified icaD gene is located between icaA
and icaB and overlaps both genes.
N-Acetylglucosamine oligomers produced by IcaAD reached a
maximal length of 20 residues. Only when icaA and
icaD were expressed together with icaC were
oligomer chains that react with PIA-specific antiserum synthesized.
IcaA and IcaD are located in the cytoplasmic membrane, and IcaC also
has all the structural features of an integral membrane protein. These results indicate a close interaction between IcaA, IcaD, and IcaC. Tunicamycin and bacitracin did not affect the in vitro
synthesis of PIA intermediates or the complete PIA biosynthesis
in vivo, suggesting that a undecaprenyl phosphate carrier
is not involved. IcaAD represents a novel protein combination among
Characterization of the
N-Acetylglucosaminyltransferase Activity Involved in the
Biosynthesis of the Staphylococcus epidermidis
Polysaccharide Intercellular Adhesin
,, and
Mikrobielle Genetik,
-glycosyltransferases.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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S. E. Cramton, C. Gerke, N. F. Schnell, W. W. Nichols, and F. Gotz The Intercellular Adhesion (ica) Locus Is Present in Staphylococcus aureus and Is Required for Biofilm Formation Infect. Immun., October 1, 1999; 67(10): 5427 - 5433. [Abstract] [Full Text] [PDF]
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 D. McKenney, K. L. Pouliot, Y. Wang, V. Murthy, M. Ulrich, G. Döring, J. C. Lee, D. A. Goldmann, and G. B. Pier Broadly Protective Vaccine for Staphylococcus aureus Based on an in Vivo-Expressed Antigen Science, May 28, 1999; 284(5419): 1523 - 1527. [Abstract] [Full Text]
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M. E. Rupp, J. S. Ulphani, P. D. Fey, K. Bartscht, and D. Mack Characterization of the Importance of Polysaccharide Intercellular Adhesin/Hemagglutinin of Staphylococcus epidermidis in the Pathogenesis of Biomaterial-Based Infection in a Mouse Foreign Body Infection Model Infect. Immun., May 1, 1999; 67(5): 2627 - 2632. [Abstract] [Full Text] [PDF]
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M. E. Rupp, J. S. Ulphani, P. D. Fey, and D. Mack Characterization of Staphylococcus epidermidis Polysaccharide Intercellular Adhesin/Hemagglutinin in the Pathogenesis of Intravascular Catheter-Associated Infection in a Rat Model Infect. Immun., May 1, 1999; 67(5): 2656 - 2659. [Abstract] [Full Text] [PDF]
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D. Mack, J. Riedewald, H. Rohde, T. Magnus, H. H. Feucht, H.-A. Elsner, R. Laufs, and M. E. Rupp Essential Functional Role of the Polysaccharide Intercellular Adhesin of Staphylococcus epidermidis in Hemagglutination Infect. Immun., February 1, 1999; 67(2): 1004 - 1008. [Abstract] [Full Text] [PDF]
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D. McKenney, J. Hubner, E. Muller, Y. Wang, D. A. Goldmann, and G. B. Pier The ica Locus of Staphylococcus epidermidis Encodes Production of the Capsular Polysaccharide/Adhesin Infect. Immun., October 1, 1998; 66(10): 4711 - 4720. [Abstract] [Full Text] [PDF]
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