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J Biol Chem, Vol. 273, Issue 29, 18610-18616, July 17, 1998

The Basolateral Localization Signal of the Follicle-stimulating Hormone Receptor

Isabelle Beau, Marie-Thérèse Groyer-Picard, André Le Bivic^§, Brigitte Vannier, Hugues Loosfelt, Edwin Milgrom, and Micheline Misrahi

From  INSERM Unité 135, Hormones Gènes et Reproduction, Institut Fédératif de Recherche 21, Hôpital Bicêtre, Assistance Publique Hopitaux de Paris, 94275 Le Kremlin Bicêtre, France and ^§ Laboratoire de Génétique et Physiologie du Développement, Unité Mixte de Recherche 9943, Faculté des Sciences Luminy, 13288 Marseille, France

The follicle-stimulating hormone receptor (FSHR) is physiologically localized in the basolateral compartment of the membrane of Sertoli cells. This localization is also observed when the receptor is experimentally expressed in Madin-Darby canine kidney cells. We thus used in vitro mutagenesis and transfection into these polarized cells to delineate the basolateral localization signal of the receptor.

The signal was localized in the C-terminal tail of the intracellular domain (amino acids 678-691) at a marked distance of the membrane. Mutation of individual amino acids highlighted the importance of Tyr⁶⁸⁴ and Leu⁶⁸⁹. The 14-amino acid sequence was grafted onto the p75 neurotrophin receptor and redirected this apical protein to the basolateral cell membrane compartment. Deletion of amino acids 677-695 did not modify the internalization of the FSHR, showing that the basolateral localization signal of the FSHR is not colinear with its internalization signal.

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