| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Vol. 273, Issue 3, 1453-1461, January 16, 1998
From the The largest subunit of the replication protein A
(RPA) contains an evolutionarily conserved zinc finger motif that lies
outside of the domains required for binding to single-stranded DNA or forming the RPA holocomplex. In previous studies, we showed that a
point mutation in this motif (RPAm) cannot support
SV40 DNA replication. We have now investigated the role of this motif
in several steps of DNA replication and in two DNA repair pathways.
RPAm associates with T antigen, assists the unwinding of
double-stranded DNA at an origin of replication, stimulates DNA
polymerases
The Evolutionarily Conserved Zinc Finger Motif in the Largest
Subunit of Human Replication Protein A Is Required for DNA Replication
and Mismatch Repair but Not for Nucleotide Excision Repair
,
Department of Pathology, Brigham and
Women's Hospital, Harvard Medical School,
Boston, Massachusetts 02115, the § Imperial Cancer
Research Fund, Clare Hall Laboratories, South Mimms,
Herts, EN6 3LD, United Kingdom, and the ¶ Charles A. Dana
Division of Human Cancer Genetics, Dana-Farber Cancer Institute,
Boston, Massachusetts 02115
and 
, and supports the formation of the initial
short Okazaki fragments. However, the synthesis of a leading strand and
later Okazaki fragments is impaired. In contrast, RPAm can
function well during the incision step of nucleotide excision repair
and in a full repair synthesis reaction, with either UV-damaged or
cisplatin-adducted DNA. Two deletion mutants of the Rpa1 subunit (eliminating amino acids 1-278 or 222-411) were not functional in
nucleotide excision repair. We report for the first time that wild type
RPA is required for a mismatch repair reaction in vitro. Neither the deletion mutants nor RPAm can support this
reaction. Therefore, the zinc finger of the largest subunit of RPA is
required for a function that is essential for DNA replication and
mismatch repair but not for nucleotide excision repair.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. W. Anantha, V. M. Vassin, and J. A. Borowiec Sequential and Synergistic Modification of Human RPA Stimulates Chromosomal DNA Repair J. Biol. Chem., December 7, 2007; 282(49): 35910 - 35923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Shell, C. D. Putnam, and R. D. Kolodner Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins PNAS, June 26, 2007; 104(26): 10956 - 10961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Modrich Mechanisms in Eukaryotic Mismatch Repair J. Biol. Chem., October 13, 2006; 281(41): 30305 - 30309. [Full Text] [PDF]
|
|
|
|
|
|
S. Guo, Y. Zhang, F. Yuan, Y. Gao, L. Gu, I. Wong, and G.-M. Li Regulation of Replication Protein A Functions in DNA Mismatch Repair by Phosphorylation J. Biol. Chem., August 4, 2006; 281(31): 21607 - 21616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Daikoku, A. Kudoh, Y. Sugaya, S. Iwahori, N. Shirata, H. Isomura, and T. Tsurumi Postreplicative Mismatch Repair Factors Are Recruited to Epstein-Barr Virus Replication Compartments J. Biol. Chem., April 21, 2006; 281(16): 11422 - 11430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Lin, J. B. Robbins, E. K. D. Nyannor, Y.-H. Chen, and I. K. O. Cann A CCCH Zinc Finger Conserved in a Replication Protein A Homolog Found in Diverse Euryarchaeotes J. Bacteriol., December 1, 2005; 187(23): 7881 - 7889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Clark and T. A. Kunkel Cadmium Inhibits the Functions of Eukaryotic MutS Complexes J. Biol. Chem., December 24, 2004; 279(52): 53903 - 53906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Corrette-Bennett, C. Borgeson, D. Sommer, P. M. J. Burgers, and R. S. Lahue DNA polymerase {delta}, RFC and PCNA are required for repair synthesis of large looped heteroduplexes in Saccharomyces cerevisiae Nucleic Acids Res., December 1, 2004; 32(21): 6268 - 6275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Ellison, J. Lofing, and G. A. Bitter Human MutL homolog (MLH1) function in DNA mismatch repair: a prospective screen for missense mutations in the ATPase domain Nucleic Acids Res., October 8, 2004; 32(18): 5321 - 5338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Yuan, L. Gu, S. Guo, C. Wang, and G.-M. Li Evidence for Involvement of HMGB1 Protein in Human DNA Mismatch Repair J. Biol. Chem., May 14, 2004; 279(20): 20935 - 20940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guo, S. R. Presnell, F. Yuan, Y. Zhang, L. Gu, and G.-M. Li Differential Requirement for Proliferating Cell Nuclear Antigen in 5' and 3' Nick-directed Excision in Human Mismatch Repair J. Biol. Chem., April 23, 2004; 279(17): 16912 - 16917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Robbins, M. C. Murphy, B. A. White, R. I. Mackie, T. Ha, and I. K. O. Cann Functional Analysis of Multiple Single-stranded DNA-binding Proteins from Methanosarcina acetivorans and Their Effects on DNA Synthesis by DNA Polymerase BI J. Biol. Chem., February 20, 2004; 279(8): 6315 - 6326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kantake, T. Sugiyama, R. D. Kolodner, and S. C. Kowalczykowski The Recombination-deficient Mutant RPA (rfa1-t11) Is Displaced Slowly from Single-stranded DNA by Rad51 Protein J. Biol. Chem., June 20, 2003; 278(26): 23410 - 23417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dabrowski, M. Olszewski, R. Piatek, A. Brillowska-Dabrowska, G. Konopa, and J. Kur Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus - new arrangement of binding domains Microbiology, October 1, 2002; 148(10): 3307 - 3315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Miturski, M. Bogusiewicz, C. Ciotta, M. Bignami, M. Gogacz, and D. Burnouf Mismatch Repair Genes and Microsatellite Instability as Molecular Markers for Gynecological Cancer Detection Experimental Biology and Medicine, September 1, 2002; 227(8): 579 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gu, J. Wu, B.-B. Zhu, and G.-M. Li Deficiency of a novel mismatch repair activity in a bladder tumor cell line Nucleic Acids Res., July 1, 2002; 30(13): 2758 - 2763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Genschel, L. R. Bazemore, and P. Modrich Human Exonuclease I Is Required for 5' and 3' Mismatch Repair J. Biol. Chem., April 5, 2002; 277(15): 13302 - 13311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ramilo, L. Gu, S. Guo, X. Zhang, S. M. Patrick, J. J. Turchi, and G.-M. Li Partial Reconstitution of Human DNA Mismatch Repair In Vitro: Characterization of the Role of Human Replication Protein A Mol. Cell. Biol., April 1, 2002; 22(7): 2037 - 2046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Grandi, M. Eltsov, I. Nielsen, and I. Raska DNA double-strand breaks induce formation of RP-A/Ku foci on in vitro reconstituted Xenopus sperm nuclei J. Cell Sci., March 11, 2002; 114(18): 3345 - 3357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pedrazzi, C. Perrera, H. Blaser, P. Kuster, G. Marra, S. L. Davies, G.-H. Ryu, R. Freire, I. D. Hickson, J. Jiricny, et al. Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1 Nucleic Acids Res., November 1, 2001; 29(21): 4378 - 4386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Amin, M.-N. Nguyen, S. Oh, and R. D. Kolodner exo1-Dependent Mutator Mutations: Model System for Studying Functional Interactions in Mismatch Repair Mol. Cell. Biol., August 1, 2001; 21(15): 5142 - 5155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. Kleczkowska, G. Marra, T. Lettieri, and J. Jiricny hMSH3 and hMSH6 interact with PCNA and colocalize with it to replication foci Genes & Dev., March 15, 2001; 15(6): 724 - 736. [Abstract] [Full Text]
|
|
|
|
|
|
C. Chen, B. J. Merrill, P. J. Lau, C. Holm, and R. D. Kolodner Saccharomyces cerevisiae pol30 (Proliferating Cell Nuclear Antigen) Mutations Impair Replication Fidelity and Mismatch Repair Mol. Cell. Biol., November 1, 1999; 19(11): 7801 - 7815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Park, M. Wang, S.-J. Park, and S.-H. Lee Zinc Finger of Replication Protein A, a Non-DNA Binding Element, Regulates Its DNA Binding Activity through Redox J. Biol. Chem., October 8, 1999; 274(41): 29075 - 29080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Brill and S. Bastin-Shanower Identification and Characterization of the Fourth Single-Stranded-DNA Binding Domain of Replication Protein A Mol. Cell. Biol., December 1, 1998; 18(12): 7225 - 7234. [Abstract] [Full Text]
|
|
|
|
|
|
V. N. Podust, N. Tiwari, S. Stephan, and E. Fanning Replication Factor C Disengages from Proliferating Cell Nuclear Antigen (PCNA) upon Sliding Clamp Formation, and PCNA Itself Tethers DNA Polymerase delta to DNA J. Biol. Chem., November 27, 1998; 273(48): 31992 - 31999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. DeMott, S. Zigman, and R. A. Bambara Replication Protein A Stimulates Long Patch DNA Base Excision Repair J. Biol. Chem., October 16, 1998; 273(42): 27492 - 27498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Otrin, I. Kuraoka, T. Nardo, M. McLenigan, A. P. M. Eker, M. Stefanini, A. S. Levine, and R. D. Wood Relationship of the Xeroderma Pigmentosum Group E DNA Repair Defect to the Chromatin and DNA Binding Proteins UV-DDB and Replication Protein A Mol. Cell. Biol., June 1, 1998; 18(6): 3182 - 3190. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Umezu, N. Sugawara, C. Chen, J. E. Haber, and R. D. Kolodner Genetic Analysis of Yeast RPA1 Reveals Its Multiple Functions in DNA Metabolism Genetics, March 1, 1998; 148(3): 989 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bochkareva, S. Korolev, and A. Bochkarev The Role for Zinc in Replication Protein A J. Biol. Chem., August 25, 2000; 275(35): 27332 - 27338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Komori and Y. Ishino Replication Protein A in Pyrococcus furiosus Is Involved in Homologous DNA Recombination J. Biol. Chem., July 6, 2001; 276(28): 25654 - 25660. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
