| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Vol. 273, Issue 3, 1534-1541, January 16, 1998
From the Hypoxanthine-guanine
phosphoribosyltransferase (HGPRT) is a key enzyme in the purine
salvage pathway of many protozoan parasites. The predicted amino acid
sequences of certain HGPRT proteins from parasites of the
Trypanosomatidae family reveal a COOH-terminal tripeptide signal that
is consistent with the degenerate topogenic signal targeting proteins
to the glycosome, a fuel-metabolizing microbody unique to these
parasites. To determine definitively the intracellular milieu of HGPRT
in these pathogens, polyclonal antiserum to the purified recombinant
HGPRT from Leishmania donovani was generated in rabbits,
and confocal and immunoelectron microscopy were employed to establish
that the L. donovani HGPRT is localized exclusively to the
glycosome. No HGPRT protein was detected in
Localization and Targeting of the Leishmania donovani
Hypoxanthine-Guanine Phosphoribosyltransferase to the
Glycosome
,,,
Department of Biochemistry and Molecular
Biology and the § Department of Pathology, Oregon Health
Sciences University, Portland, Oregon 97201-3098
hgprt null
mutants in which both alleles of the HGPRT locus had been
replaced by a drug-resistance cassette. Transfectants of the
hgprt knockout strain in which a wild-type
HGPRT was amplified on an expression plasmid contained
augmented amounts of HGPRT, all of which was localized to the
glycosome. hgprt transfectants containing amplified
copies of a mutated HGPRT construct in which the Ser-Lys-Val
COOH-terminal targeting signal had been deleted expressed HGPRT
throughout the parasite, including subcellular organelles such as the
nucleus and flagellum. These data demonstrate that the L. donovani HGPRT is compartmentalized exclusively within the
glycosome and that the COOH-terminal tripeptide of the protein is
necessary to achieve targeting to this organelle.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Boitz and B. Ullman A Conditional Mutant Deficient in Hypoxanthine-guanine Phosphoribosyltransferase and Xanthine Phosphoribosyltransferase Validates the Purine Salvage Pathway of Leishmania donovani J. Biol. Chem., June 9, 2006; 281(23): 16084 - 16089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zufferey and C. B. Mamoun Leishmania major Expresses a Single Dihydroxyacetone Phosphate Acyltransferase Localized in the Glycosome, Important for Rapid Growth and Survival at High Cell Density and Essential for Virulence J. Biol. Chem., March 24, 2006; 281(12): 7952 - 7959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zheng, K. D. Butler, R. K. Tweten, and K. Mensa-Wilmot Endosomes, Glycosomes, and Glycosylphosphatidylinositol Catabolism in Leishmania major J. Biol. Chem., October 1, 2004; 279(40): 42106 - 42113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. Madrid, G. De Crescenzo, S. Wang, and A. Jardim Modulation of the Leishmania donovani Peroxin 5 Quaternary Structure by Peroxisomal Targeting Signal 1 Ligands Mol. Cell. Biol., September 1, 2004; 24(17): 7331 - 7344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Roberts, M. J. Tancer, M. R. Polinsky, K. M. Gibson, O. Heby, and B. Ullman Arginase Plays a Pivotal Role in Polyamine Precursor Metabolism in Leishmania: CHARACTERIZATION OF GENE DELETION MUTANTS J. Biol. Chem., May 28, 2004; 279(22): 23668 - 23678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zufferey, S. Allen, T. Barron, D. R. Sullivan, P. W. Denny, I. C. Almeida, D. F. Smith, S. J. Turco, M. A. J. Ferguson, and S. M. Beverley Ether Phospholipids and Glycosylinositolphospholipids Are Not Required for Amastigote Virulence or for Inhibition of Macrophage Activation by Leishmania major J. Biol. Chem., November 7, 2003; 278(45): 44708 - 44718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Plewes, S. D. Barr, and L. Gedamu Iron Superoxide Dismutases Targeted to the Glycosomes of Leishmania chagasi Are Important for Survival Infect. Immun., October 1, 2003; 71(10): 5910 - 5920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Furuya, P. Kessler, A. Jardim, A. Schnaufer, C. Crudder, and M. Parsons Glucose is toxic to glycosome-deficient trypanosomes PNAS, October 29, 2002; 99(22): 14177 - 14182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Wilkinson, D. J. Meyer, M. C. Taylor, E. V. Bromley, M. A. Miles, and J. M. Kelly The Trypanosoma cruzi Enzyme TcGPXI Is a Glycosomal Peroxidase and Can Be Linked to Trypanothione Reduction by Glutathione or Tryparedoxin J. Biol. Chem., May 3, 2002; 277(19): 17062 - 17071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Fling, R. A. Sutherland, L. N. Steele, B. Hess, S. E. F. D'Orazio, J.-F. Maisonneuve, M. F. Lampe, P. Probst, and M. N. Starnbach CD8+ T cells recognize an inclusion membrane-associated protein from the vacuolar pathogen Chlamydia trachomatis PNAS, January 30, 2001; 98(3): 1160 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jardim, W. Liu, E. Zheleznova, and B. Ullman Peroxisomal Targeting Signal-1 Receptor Protein PEX5 from Leishmania donovani. MOLECULAR, BIOCHEMICAL, AND IMMUNOCYTOCHEMICAL CHARACTERIZATION J. Biol. Chem., April 28, 2000; 275(18): 13637 - 13644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jardim, S. E. Bergeson, S. Shih, N. Carter, R. W. Lucas, G. Merlin, P. J. Myler, K. Stuart, and B. Ullman Xanthine Phosphoribosyltransferase from Leishmania donovani. MOLECULAR CLONING, BIOCHEMICAL CHARACTERIZATION, AND GENETIC ANALYSIS J. Biol. Chem., November 26, 1999; 274(48): 34403 - 34410. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
