HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kimura, M. Articles by Yamaguchi, I. Search for Related Content PubMed PubMed Citation Articles by Kimura, M. Articles by Yamaguchi, I. Social Bookmarking                      What's this?

Vol. 273, Issue 3, 1654-1661, January 16, 1998

Trichothecene 3-O-Acetyltransferase Protects Both the Producing Organism and Transformed Yeast from Related Mycotoxins
CLONING AND CHARACTERIZATION OF Tri101

Makoto Kimura, Isao Kaneko, Masami Komiyama, Akira Takatsuki^¶, Hiroyuki Koshino, Katsuyoshi Yoneyama, and Isamu Yamaguchi

From the  Microbial Toxicology Laboratory, the ^¶ Animal and Cellular Systems Laboratory, and the  Division of Molecular Characterization, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama 351-01, Japan

Trichothecene mycotoxins such as deoxynivalenol, 4,15-diacetoxyscirpenol, and T-2 toxin, are potent protein synthesis inhibitors for eukaryotic organisms. The 3-O-acetyl derivatives of these toxins were shown to reduce their in vitro activity significantly as assessed by assays using a rabbit reticulocyte translation system. The results suggested that the introduction of an O-acetyl group at the C-3 position in the biosynthetic pathway works as a resistance mechanism for Fusarium species that produce t-type trichothecenes (trichothecenes synthesized via the precursor trichotriol).

A gene responsible for the 3-O-acetylation reaction, Tri101, has been successfully cloned from a Fusarium graminearum cDNA library that was designed to be expressed in Schizosaccharomyces pombe. Fission yeast transformants were selected for their ability to grow in the presence of T-2 toxin, and this strategy allowed isolation of 25 resistant clones, all of which contained a cDNA for Tri101. This is the first drug-inactivating O-acetyltransferase gene derived from antibiotic-producing organisms. The open reading frame of Tri101 codes for a polypeptide of 451 amino acid residues, which shows no similarity to any other proteins reported so far. TRI101 from recombinant Escherichia coli catalyzes O-acetylation of the trichothecene ring specifically at the C-3 position in an acetyl-CoA-dependent manner. By using the Tri101 cDNA as a probe, two least overlapping cosmid clones that cover a region of 70 kilobase pairs have been isolated from the genome of F. graminearum. Other trichothecene biosynthetic genes, Tri4, Tri5, and Tri6, were not clustered in the region covered by these cosmid clones. These new cosmid clones are considered to be located in other parts of the large biosynthetic gene cluster and might be useful for the study of trichothecene biosynthesis.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				P. Bregitzer, L. S. Dahleen, S. Neate, P. Schwarz, and M. Manoharan A Single Backcross Effectively Eliminates Agronomic and Quality Alterations Caused by Somaclonal Variation in Transgenic Barley Crop Sci., March 19, 2008; 48(2): 471 - 479. [Abstract] [Full Text] [PDF]

				G. S. Garvey, S. P. McCormick, and I. Rayment Structural and Functional Characterization of the TRI101 Trichothecene 3-O-Acetyltransferase from Fusarium sporotrichioides and Fusarium graminearum: KINETIC INSIGHTS TO COMBATING FUSARIUM HEAD BLIGHT J. Biol. Chem., January 18, 2008; 283(3): 1660 - 1669. [Abstract] [Full Text] [PDF]

				T. Igawa, N. Takahashi-Ando, N. Ochiai, S. Ohsato, T. Shimizu, T. Kudo, I. Yamaguchi, and M. Kimura Reduced Contamination by the Fusarium Mycotoxin Zearalenone in Maize Kernels through Genetic Modification with a Detoxification Gene Appl. Envir. Microbiol., March 1, 2007; 73(5): 1622 - 1629. [Abstract] [Full Text] [PDF]

				S. Hewald, U. Linne, M. Scherer, M. A. Marahiel, J. Kamper, and M. Bolker Identification of a Gene Cluster for Biosynthesis of Mannosylerythritol Lipids in the Basidiomycetous Fungus Ustilago maydis Appl. Envir. Microbiol., August 1, 2006; 72(8): 5469 - 5477. [Abstract] [Full Text] [PDF]

				T. Tokai, M. Fujimura, H. Inoue, T. Aoki, K. Ohta, T. Shibata, I. Yamaguchi, and M. Kimura Concordant evolution of trichothecene 3-O-acetyltransferase and an rDNA species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi Microbiology, February 1, 2005; 151(2): 509 - 519. [Abstract] [Full Text] [PDF]

				T. Igawa, T. Ochiai-Fukuda, N. Takahashi-Ando, S. Ohsato, T. Shibata, I. Yamaguchi, and M. Kimura New TAXI-type Xylanase Inhibitor Genes are Inducible by Pathogens and Wounding in Hexaploid Wheat Plant Cell Physiol., October 15, 2004; 45(10): 1347 - 1360. [Abstract] [Full Text] [PDF]

				S. P. McCormick, L. J. Harris, N. J. Alexander, T. Ouellet, A. Saparno, S. Allard, and A. E. Desjardins Tri1 in Fusarium graminearum Encodes a P450 Oxygenase Appl. Envir. Microbiol., April 1, 2004; 70(4): 2044 - 2051. [Abstract] [Full Text] [PDF]

				A. W. Peplow, A. G. Tag, G. F. Garifullina, and M. N. Beremand Identification of New Genes Positively Regulated by Tri10 and a Regulatory Network for Trichothecene Mycotoxin Production Appl. Envir. Microbiol., May 1, 2003; 69(5): 2731 - 2736. [Abstract] [Full Text] [PDF]

				M. Kimura, T. Tokai, G. Matsumoto, M. Fujimura, H. Hamamoto, K. Yoneyama, T. Shibata, and I. Yamaguchi Trichothecene Nonproducer Gibberella Species Have Both Functional and Nonfunctional 3-O-Acetyltransferase Genes Genetics, February 1, 2003; 163(2): 677 - 684. [Abstract] [Full Text] [PDF]

				T. K. Mitchell, W. S. Chilton, and M. E. Daub Biodegradation of the Polyketide Toxin Cercosporin Appl. Envir. Microbiol., September 1, 2002; 68(9): 4173 - 4181. [Abstract] [Full Text] [PDF]

				D. Baidyaroy, G. Brosch, S. Graessle, P. Trojer, and J. D. Walton Characterization of Inhibitor-Resistant Histone Deacetylase Activity in Plant-Pathogenic Fungi Eukaryot. Cell, August 1, 2002; 1(4): 538 - 547. [Abstract] [Full Text] [PDF]

				T. J. Ward, J. P. Bielawski, H. C. Kistler, E. Sullivan, and K. O'Donnell Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytopathogenic Fusarium PNAS, July 9, 2002; 99(14): 9278 - 9283. [Abstract] [Full Text] [PDF]

				S. P. McCormick and N. J. Alexander Fusarium Tri8 Encodes a Trichothecene C-3 Esterase Appl. Envir. Microbiol., June 1, 2002; 68(6): 2959 - 2964. [Abstract] [Full Text] [PDF]

				T. Lee, Y.-K. Han, K.-H. Kim, S.-H. Yun, and Y.-W. Lee Tri13 and Tri7 Determine Deoxynivalenol- and Nivalenol-Producing Chemotypes of Gibberella zeae Appl. Envir. Microbiol., May 1, 2002; 68(5): 2148 - 2154. [Abstract] [Full Text] [PDF]

				A. G. Tag, G. F. Garifullina, A. W. Peplow, C. Ake Jr., T. D. Phillips, T. M. Hohn, and M. N. Beremand A Novel Regulatory Gene, Tri10, Controls Trichothecene Toxin Production and Gene Expression Appl. Envir. Microbiol., November 1, 2001; 67(11): 5294 - 5302. [Abstract] [Full Text]

				T. Lee, D.-W. Oh, H.-S. Kim, J. Lee, Y.-H. Kim, S.-H. Yun, and Y.-W. Lee Identification of Deoxynivalenol- and Nivalenol-Producing Chemotypes of Gibberella zeae by Using PCR Appl. Envir. Microbiol., July 1, 2001; 67(7): 2966 - 2972. [Abstract] [Full Text] [PDF]

				K. O'Donnell, H. C. Kistler, B. K. Tacke, and H. H. Casper Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab PNAS, June 23, 2000; (2000) 130193297. [Abstract] [Full Text]

				L. Chen, S. P. McCormick, and T. M. Hohn Altered Regulation of 15-Acetyldeoxynivalenol Production in Fusarium graminearum Appl. Envir. Microbiol., May 1, 2000; 66(5): 2062 - 2065. [Abstract] [Full Text]

				S. P. McCormick, N. J. Alexander, S. E. Trapp, and T. M. Hohn Disruption of TRI101, the Gene Encoding Trichothecene 3-O-Acetyltransferase, from Fusarium sporotrichioides Appl. Envir. Microbiol., December 1, 1999; 65(12): 5252 - 5256. [Abstract] [Full Text]

				H. Suzuki, T. Nakayama, K. Yonekura-Sakakibara, Y. Fukui, N. Nakamura, M. Nakao, Y. Tanaka, M.-a. Yamaguchi, T. Kusumi, and T. Nishino Malonyl-CoA:Anthocyanin 5-O-Glucoside-6'''-O-Malonyltransferase from Scarlet Sage (Salvia splendens) Flowers. ENZYME PURIFICATION, GENE CLONING, EXPRESSION, AND CHARACTERIZATION J. Biol. Chem., December 21, 2001; 276(52): 49013 - 49019. [Abstract] [Full Text] [PDF]

				K. O'Donnell, H. C. Kistler, B. K. Tacke, and H. H. Casper Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab PNAS, July 5, 2000; 97(14): 7905 - 7910. [Abstract] [Full Text] [PDF]