![]()
|
|
||||||||
Vol. 273, Issue 3, 1689-1698, January 16, 1998
From the To survive in a mechanically active environment,
cells must adapt to variations of applied membrane tension. A
collagen-coated magnetic bead model was used to apply forces directly
to the actin cytoskeleton through integrin receptors. We demonstrate
here that by a calcium-dependent mechanism, human fibroblasts reinforce locally their connection with extracellular adhesion sites by inducing
actin assembly and by recruiting actin-binding protein 280 (ABP-280)
into cortical adhesion complexes. ABP-280 was phosphorylated on serine
residues as a result of force application. This phosphorylation and the
force-induced actin reorganization were largely abrogated by inhibitors
of protein kinase C. In a human melanoma cell line that does not
express ABP-280, actin accumulation could not be induced by force,
whereas in stable transfectants expressing ABP-280, force-induced actin
accumulation was similar to human fibroblasts. Cortical actin assembly
played a role in regulating the activity of stretch-activated,
calcium-permeable channels (SAC) since sustained force application
desensitized SAC to subsequent force applications, and the decrease in
stretch sensitivity was reversed after treatment with cytochalasin D. ABP-280-deficient cells showed a >90% increase in cell death compared
with ABP-280+ve cells after force application. We conclude that ABP-280
plays an important role in mechanoprotection by reinforcing the
membrane cortex and desensitizing SACs.
The Role of Actin-binding Protein 280 in
Integrin-dependent Mechanoprotection
,
,
,
, and
MRC Group in Periodontal Physiology, Faculty
of Dentistry, University of Toronto,
Toronto, Ontario M5S 1A8, Canada, the ¶ Brigham and Women's
Hospital, Harvard Medical School, Boston, Massachusetts 02115, and
the
Faculty of Medicine, Department of Medicine, University of
Toronto, Toronto, Ontario M5S 1A8, Canada
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
Y. J. Jeon, J. S. Choi, J. Y. Lee, K. R. Yu, S. H. Ka, Y. Cho, E.-J. Choi, S. H. Baek, J. H. Seol, D. Park, et al. Filamin B Serves as a Molecular Scaffold for Type I Interferon-induced c-Jun NH2-terminal Kinase Signaling Pathway Mol. Biol. Cell, December 1, 2008; 19(12): 5116 - 5130. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. M. Jackson, M. J. Jaasma, R. Y. Tang, and T. M. Keaveny Mechanical loading by fluid shear is sufficient to alter the cytoskeletal composition of osteoblastic cells Am J Physiol Cell Physiol, October 1, 2008; 295(4): C1007 - C1015. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Hirata, H. Tatsumi, and M. Sokabe Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner J. Cell Sci., September 1, 2008; 121(17): 2795 - 2804. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. C. Mak, Q. Wang, C. Laschinger, W. Lee, D. Ron, H. P. Harding, R. J. Kaufman, D. Scheuner, R. C. Austin, and C. A. McCulloch Novel Function of PERK as a Mediator of Force-induced Apoptosis J. Biol. Chem., August 22, 2008; 283(34): 23462 - 23472. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. R. Quinlan, N. G. Docherty, R. W. G. Watson, and J. M. Fitzpatrick Exploring mechanisms involved in renal tubular sensing of mechanical stretch following ureteric obstruction Am J Physiol Renal Physiol, July 1, 2008; 295(1): F1 - F11. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Tandon, I. Levental, C. Huang, F. J. Byfield, J. Ziembicki, J. R. Schelling, L. A. Bruggeman, J. R. Sedor, P. A. Janmey, and R. T. Miller HIV infection changes glomerular podocyte cytoskeletal composition and results in distinct cellular mechanical properties Am J Physiol Renal Physiol, February 1, 2007; 292(2): F701 - F710. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Deshmukh, V. G. Coffey, Z. Zhong, A. V. Chibalin, J. A. Hawley, and J. R. Zierath Exercise-Induced Phosphorylation of the Novel Akt Substrates AS160 and Filamin A in Human Skeletal Muscle Diabetes, June 1, 2006; 55(6): 1776 - 1782. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. F. Coughlin, M. Puig-de-Morales, P. Bursac, M. Mellema, E. Millet, and J. J. Fredberg Filamin-A and Rheological Properties of Cultured Melanoma Cells Biophys. J., March 15, 2006; 90(6): 2199 - 2205. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. J. Maxwell, S. M. Dopheide, S. J. Turner, and S. P. Jackson Shear Induces a Unique Series of Morphological Changes in Translocating Platelets: Effects of Morphology on Translocation Dynamics Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 663 - 669. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. D. Matthews, D. R. Overby, R. Mannix, and D. E. Ingber Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels J. Cell Sci., February 1, 2006; 119(3): 508 - 518. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. Klaile, M. M. Muller, C. Kannicht, B. B. Singer, and L. Lucka CEACAM1 functionally interacts with filamin A and exerts a dual role in the regulation of cell migration J. Cell Sci., December 1, 2005; 118(23): 5513 - 5524. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Wang, J. Fan, C. Laschinger, P. D. Arora, A. Kapus, A. Seth, and C. A. McCulloch Smooth Muscle Actin Determines Mechanical Force-induced p38 Activation J. Biol. Chem., February 25, 2005; 280(8): 7273 - 7284. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. P. Helmke Molecular Control of Cytoskeletal Mechanics by Hemodynamic Forces Physiology, February 1, 2005; 20(1): 43 - 53. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. T. Charras, B. A. Williams, S. M. Sims, and M. A. Horton Estimating the Sensitivity of Mechanosensitive Ion Channels to Membrane Strain and Tension Biophys. J., October 1, 2004; 87(4): 2870 - 2884. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Deng, N. J. Fairbank, B. Fabry, P. G. Smith, and G. N. Maksym Localized mechanical stress induces time-dependent actin cytoskeletal remodeling and stiffening in cultured airway smooth muscle cells Am J Physiol Cell Physiol, August 1, 2004; 287(2): C440 - C448. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Tseng, K. M. An, O. Esue, and D. Wirtz The Bimodal Role of Filamin in Controlling the Architecture and Mechanics of F-actin Networks J. Biol. Chem., January 16, 2004; 279(3): 1819 - 1826. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Giannone, G. Jiang, D. H. Sutton, D. R. Critchley, and M. P. Sheetz Talin1 is critical for force-dependent reinforcement of initial integrin-cytoskeleton bonds but not tyrosine kinase activation J. Cell Biol., October 27, 2003; 163(2): 409 - 419. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. J. Gunst and J. J. Fredberg The first three minutes: smooth muscle contraction, cytoskeletal events, and soft glasses J Appl Physiol, July 1, 2003; 95(1): 413 - 425. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. N. Fass and D. J. Odde Tensile Force-Dependent Neurite Elicitation via Anti-{beta}1 Integrin Antibody-Coated Magnetic Beads Biophys. J., July 1, 2003; 85(1): 623 - 636. [Abstract] [Full Text] [PDF] |
||||
![]() |
U. Tigges, B. Koch, J. Wissing, B. M. Jockusch, and W. H. Ziegler The F-actin Cross-linking and Focal Adhesion Protein Filamin A Is a Ligand and in Vivo Substrate for Protein Kinase C{alpha} J. Biol. Chem., June 20, 2003; 278(26): 23561 - 23569. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. M. Ervasti Costameres: the Achilles' Heel of Herculean Muscle J. Biol. Chem., April 11, 2003; 278(16): 13591 - 13594. [Full Text] [PDF] |
||||
![]() |
L. Vonna, A. Wiedemann, M. Aepfelbacher, and E. Sackmann Local force induced conical protrusions of phagocytic cells J. Cell Sci., March 1, 2003; 116(5): 785 - 790. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. D'Addario, P. D. Arora, R. P. Ellen, and C. A. G. McCulloch Interaction of p38 and Sp1 in a Mechanical Force-induced, beta 1 Integrin-mediated Transcriptional Circuit That Regulates the Actin-binding Protein Filamin-A J. Biol. Chem., November 27, 2002; 277(49): 47541 - 47550. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. E. Vlahakis, M. A. Schroeder, R. E. Pagano, and R. D. Hubmayr Role of Deformation-induced Lipid Trafficking in the Prevention of Plasma Membrane Stress Failure Am. J. Respir. Crit. Care Med., November 1, 2002; 166(9): 1282 - 1289. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Kainulainen, A. Pender, M. D'Addario, Y. Feng, P. Lekic, and C. A. McCulloch Cell Death and Mechanoprotection by Filamin A in Connective Tissues after Challenge by Applied Tensile Forces J. Biol. Chem., June 7, 2002; 277(24): 21998 - 22009. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Moro, R. Carrozzo, P. Veggiotti, G. Tortorella, D. Toniolo, A. Volzone, and R. Guerrini Familial periventricular heterotopia: Missense and distal truncating mutations of the FLN1 gene Neurology, March 26, 2002; 58(6): 916 - 921. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Sawada and M. P. Sheetz Force transduction by Triton cytoskeletons J. Cell Biol., February 18, 2002; 156(4): 609 - 615. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Yuan and Z. Shen Interaction with BRCA2 Suggests a Role for Filamin-1 (hsFLNa) in DNA Damage Response J. Biol. Chem., December 14, 2001; 276(51): 48318 - 48324. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. C. Reedy, B. Bullard, and J. O. Vigoreaux Flightin Is Essential for Thick Filament Assembly and Sarcomere Stability in Drosophila Flight Muscles J. Cell Biol., December 27, 2000; 151(7): 1483 - 1500. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. E. Vlahakis and R. D. Hubmayr Cellular Responses to Mechanical Stress: Invited Review: Plasma membrane stress failure in alveolar epithelial cells J Appl Physiol, December 1, 2000; 89(6): 2490 - 2496. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Petrecca, D. M. Miller, and A. Shrier Localization and Enhanced Current Density of the Kv4.2 Potassium Channel by Interaction with the Actin-Binding Protein Filamin J. Neurosci., December 1, 2000; 20(23): 8736 - 8744. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Ressler, R. T. Lee, S. H. Randell, J. M. Drazen, and R. D. Kamm Molecular responses of rat tracheal epithelial cells to transmembrane pressure Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1264 - L1272. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. G. Thompson, Y.-M. Chan, A. A. Hack, M. Brosius, M. Rajala, H. G.W. Lidov, E. M. McNally, S. Watkins, and L. M. Kunkel Filamin 2 (FLN2): A Muscle-specific Sarcoglycan Interacting Protein J. Cell Biol., January 10, 2000; 148(1): 115 - 126. [Abstract] [Full Text] [PDF] |
||||
![]() |
S Liu, D. Calderwood, and M. Ginsberg Integrin cytoplasmic domain-binding proteins J. Cell Sci., January 10, 2000; 113(20): 3563 - 3571. [Abstract] [PDF] |
||||
![]() |
A. Leonardi, H. Ellinger-Ziegelbauer, G. Franzoso, K. Brown, and U. Siebenlist Physical and Functional Interaction of Filamin (Actin-binding Protein-280) and Tumor Necrosis Factor Receptor-associated Factor 2 J. Biol. Chem., January 7, 2000; 275(1): 271 - 278. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Stahlhut and B. van Deurs Identification of Filamin as a Novel Ligand for Caveolin-1: Evidence for the Organization of Caveolin-1-associated Membrane Domains by the Actin Cytoskeleton Mol. Biol. Cell, January 1, 2000; 11(1): 325 - 337. [Abstract] [Full Text] |
||||
![]() |
M.-g. Li, M. Serr, K. Edwards, S. Ludmann, D. Yamamoto, L. G. Tilney, C. M. Field, and T. S. Hays Filamin Is Required for Ring Canal Assembly and Actin Organization during Drosophila Oogenesis J. Cell Biol., September 6, 1999; 146(5): 1061 - 1074. [Abstract] [Full Text] [PDF] |
||||
![]() |
X. Wan, P. Juranka, and C. E. Morris Activation of mechanosensitive currents in traumatized membrane Am J Physiol Cell Physiol, February 1, 1999; 276(2): C318 - C327. [Abstract] [Full Text] [PDF] |
||||
![]() |
I Korichneva and U Hammerling F-actin as a functional target for retro-retinoids: a potential role in anhydroretinol-triggered cell death J. Cell Sci., January 8, 1999; 112(15): 2521 - 2528. [Abstract] [PDF] |
||||
![]() |
J. Dai, M. P. Sheetz, X. Wan, and C. E. Morris Membrane Tension in Swelling and Shrinking Molluscan Neurons J. Neurosci., September 1, 1998; 18(17): 6681 - 6692. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. A. Browne, R. W. Johnstone, D. A. Jans, and J. A. Trapani Filamin (280-kDa Actin-binding Protein) Is a Caspase Substrate and Is Also Cleaved Directly by the Cytotoxic T Lymphocyte Protease Granzyme B during Apoptosis J. Biol. Chem., December 8, 2000; 275(50): 39262 - 39266. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Sasaki, Y. Masuda, Y. Ohta, K. Ikeda, and K. Watanabe Filamin Associates with Smads and Regulates Transforming Growth Factor-beta Signaling J. Biol. Chem., May 18, 2001; 276(21): 17871 - 17877. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Awata, C. Huang, M. E. Handlogten, and R. T. Miller Interaction of the Calcium-sensing Receptor and Filamin, a Potential Scaffolding Protein J. Biol. Chem., September 7, 2001; 276(37): 34871 - 34879. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. D'Addario, P. D. Arora, J. Fan, B. Ganss, R. P. Ellen, and C. A. G. McCulloch Cytoprotection against Mechanical Forces Delivered through beta 1 Integrins Requires Induction of Filamin A J. Biol. Chem., August 17, 2001; 276(34): 31969 - 31977. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. A. Calderwood, S. J. Shattil, and M. H. Ginsberg Integrins and Actin Filaments: Reciprocal Regulation of Cell Adhesion and Signaling J. Biol. Chem., July 21, 2000; 275(30): 22607 - 22610. [Full Text] [PDF] |
||||
![]() |
Y. Sawada and M. P. Sheetz Force transduction by Triton cytoskeletons J. Cell Biol., February 18, 2002; 156(4): 609 - 615. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |