| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 30, 18689-18692, July 24, 1998
,¶, and
From the Every kinetoplastid mRNA receives a common,
conserved leader sequence via the process of
trans-splicing. In Leishmania tarentolae the
precursor spliced leader RNA is 96 nucleotides, with a 39-nucleotide exon that is 7meG-capped and methylated on the first 4 nucleotides. trans-Splicing was inferred from the presence of tagged
leader in the high molecular weight RNA population and confirmed for accuracy by cDNA cloning. Linker scan substitutions within the exon
between positions 10 and 39 did not affect trans-splicing. The trans-splicing efficiency for three of the scan exons
was proportional to the tagged:wild type ratio in the spliced leader precursor population. Two scan leader RNAs that were efficiently spliced showed reduced methylation. Longer exons showed reduced splicing, whereas 10- or 20-base pair deletions abolished splicing. These results indicate that size, but not content, of the exon is a
constraint on the splicing process. These results, in combination with
previous data eliminating a role in transcription initiation, suggest
that translation may be the selective pressure on the leader
content.
Department of Microbiology and Immunology,
UCLA School of Medicine, Los Angeles, California 90095 and the
¶ Department of Medicine, West Los Angeles Veterans Affairs
Medical Center, Los Angeles, California 90073
This article has been cited by other articles:
|
|
 |
|
  B. Mittra, J. R. Zamudio, J. M. Bujnicki, J. Stepinski, E. Darzynkiewicz, D. A. Campbell, and N. R. Sturm The TbMTr1 Spliced Leader RNA Cap 1 2 '-O-Ribose Methyltransferase from Trypanosoma brucei Acts with Substrate Specificity J. Biol. Chem., February 8, 2008; 283(6): 3161 - 3172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Zamudio, B. Mittra, S. Foldynova-Trantirkova, G. M. Zeiner, J. Lukes, J. M. Bujnicki, N. R. Sturm, and D. A. Campbell The 2'-O-Ribose Methyltransferase for Cap 1 of Spliced Leader RNA and U1 Small Nuclear RNA in Trypanosoma brucei Mol. Cell. Biol., September 1, 2007; 27(17): 6084 - 6092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Zamudio, B. Mittra, G. M. Zeiner, M. Feder, J. M. Bujnicki, N. R. Sturm, and D. A. Campbell Complete Cap 4 Formation Is Not Required for Viability in Trypanosoma brucei Eukaryot. Cell, June 1, 2006; 5(6): 905 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Barth, A. Hury, X.-h. Liang, and S. Michaeli Elucidating the Role of H/ACA-like RNAs in trans-Splicing and rRNA Processing via RNA Interference Silencing of the Trypanosoma brucei CBF5 Pseudouridine Synthase J. Biol. Chem., October 14, 2005; 280(41): 34558 - 34568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Zeiner, R. A. Hitchcock, N. R. Sturm, and D. A. Campbell 3'-End Polishing of the Kinetoplastid Spliced Leader RNA Is Performed by SNIP, a 3'->5' Exonuclease with a Motley Assortment of Small RNA Substrates Mol. Cell. Biol., December 1, 2004; 24(23): 10390 - 10396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Zeiner, S. Foldynova, N. R. Sturm, J. Lukes, and D. A. Campbell SmD1 Is Required for Spliced Leader RNA Biogenesis Eukaryot. Cell, February 1, 2004; 3(1): 241 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Zeiner, N. R. Sturm, and D. A. Campbell The Leishmania tarentolae Spliced Leader Contains Determinants for Association with Polysomes J. Biol. Chem., October 3, 2003; 278(40): 38269 - 38275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-h. Liang, A. Haritan, S. Uliel, and S. Michaeli trans and cis Splicing in Trypanosomatids: Mechanism, Factors, and Regulation Eukaryot. Cell, October 1, 2003; 2(5): 830 - 840. [Full Text] [PDF]
|
|
|
|
|
|
G. M. Zeiner, N. R. Sturm, and D. A. Campbell Exportin 1 Mediates Nuclear Export of the Kinetoplastid Spliced Leader RNA Eukaryot. Cell, April 1, 2003; 2(2): 222 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mandelboim, C. L. Estrano, C. Tschudi, E. Ullu, and S. Michaeli On the Role of Exon and Intron Sequences in trans-Splicing Utilization and cap 4 Modification of the Trypanosomatid Leptomonas collosoma SL RNA J. Biol. Chem., September 13, 2002; 277(38): 35210 - 35218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Evans and T. Blumenthal trans Splicing of Polycistronic Caenorhabditis elegans Pre-mRNAs: Analysis of the SL2 RNA Mol. Cell. Biol., September 15, 2000; 20(18): 6659 - 6667. [Abstract] [Full Text]
|
|
|
|
|
|
M. N. Schnare and M. W. Gray A Candidate U1 Small Nuclear RNA for Trypanosomatid Protozoa J. Biol. Chem., August 20, 1999; 274(34): 23691 - 23694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Sturm and D. A. Campbell The Role of Intron Structures in trans-Splicing and Cap 4 Formation for the Leishmania Spliced Leader RNA J. Biol. Chem., July 2, 1999; 274(27): 19361 - 19367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Sturm, M. C. Yu, and D. A. Campbell Transcription Termination and 3'-End Processing of the Spliced Leader RNA in Kinetoplastids Mol. Cell. Biol., February 1, 1999; 19(2): 1595 - 1604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-x. Xu, L. Liu, and S. Michaeli Functional Analyses of Positions across the 5' Splice Site of the Trypanosomatid Spliced Leader RNA. IMPLICATIONS FOR BASE-PAIR INTERACTION WITH U5 AND U6 snRNAs J. Biol. Chem., September 1, 2000; 275(36): 27883 - 27892. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
