HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yeagley, D. Articles by Quinn, P. G. Search for Related Content PubMed PubMed Citation Articles by Yeagley, D. Articles by Quinn, P. G. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 30, 18743-18750, July 24, 1998

A Tripartite Array of Transcription Factor Binding Sites Mediates cAMP Induction of Phosphoenolpyruvate Carboxykinase Gene Transcription and Its Inhibition by Insulin

From the Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033

Transcription of the phosphoenolpyruvate carboxykinase (PEPCK) gene is induced upon activation of protein kinase A by cAMP and phosphorylation of Ser-133 in the transcription factor, cAMP-response element binding protein (CREB), and this induction is inhibited by insulin. We show here that insulin does not act by dephosphorylating CREB or by affecting heterologous kinases that phosphorylate Ser-129 or Ser-142 in CREB. In addition, insulin inhibition of minimal PEPCK promoter activity induced by CREB-GAL4 + protein kinase A was equivalent to inhibition of basal transcription, and thus cAMP-independent. On the other hand, nearly complete insulin inhibition is observed with the full PEPCK promoter (600/+69), indicating that other factors are involved. The additional promoter elements required for induction by protein kinase A lie within 271 nucleotides of the start site and correspond to putative binding sites for activator protein-1 and CAAT/enhancer-binding protein (C/EBP), first identified by Roesler et al. (Roesler, W. J., McFie, P. J., and Puttick, D. M., (1993) J. Biol. Chem. 268, 3791-3796). This tripartite array of binding sites for CREB, C/EBP, and activator protein-1 (AP-1) factors forms a cAMP response unit that, together with the minimal promoter, can mediate both induction by cAMP and inhibition by insulin. Thus, for the PEPCK gene with a single CREB site, the CREB·CBP·RNA polymerase II complex cannot mediate either induction by cAMP or inhibition by insulin.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				N. Horike, H. Sakoda, A. Kushiyama, H. Ono, M. Fujishiro, H. Kamata, K. Nishiyama, Y. Uchijima, Y. Kurihara, H. Kurihara, et al. AMP-activated Protein Kinase Activation Increases Phosphorylation of Glycogen Synthase Kinase 3{beta} and Thereby Reduces cAMP-responsive Element Transcriptional Activity and Phosphoenolpyruvate Carboxykinase C Gene Expression in the Liver J. Biol. Chem., December 5, 2008; 283(49): 33902 - 33910. [Abstract] [Full Text] [PDF]

				M. R. Peluso Flavonoids Attenuate Cardiovascular Disease, Inhibit Phosphodiesterase, and Modulate Lipid Homeostasis in Adipose Tissue and Liver Experimental Biology and Medicine, September 1, 2006; 231(8): 1287 - 1299. [Abstract] [Full Text] [PDF]

				Z. Hazan-Eitan, Y. Weinstein, N. Hadad, A. Konforty, and R. Levy Induction of Fc{gamma}RIIA expression in myeloid PLB cells during differentiation depends on cytosolic phospholipase A2 activity and is regulated via activation of CREB by PGE2 Blood, September 1, 2006; 108(5): 1758 - 1766. [Abstract] [Full Text] [PDF]

				X. Li, P. A. Hansen, L. Xi, R. A. S. Chandraratna, and C. F. Burant Distinct Mechanisms of Glucose Lowering by Specific Agonists for Peroxisomal Proliferator Activated Receptor {gamma} and Retinoic Acid X Receptors J. Biol. Chem., November 18, 2005; 280(46): 38317 - 38327. [Abstract] [Full Text] [PDF]

				I. Najwer and B. Lilly Ca2+/calmodulin-dependent protein kinase IV activates cysteine-rich protein 1 through adjacent CRE and CArG elements Am J Physiol Cell Physiol, October 1, 2005; 289(4): C785 - C793. [Abstract] [Full Text] [PDF]

				D. Yeagley and P. G. Quinn 3',5'-Cyclic Adenosine Monophosphate Response Element-Binding Protein and CCAAT Enhancer-Binding Protein Are Dispensable for Insulin Inhibition of Phosphoenolpyruvate Carboxykinase Transcription and for Its Synergistic Induction by Protein Kinase A and Glucocorticoids Mol. Endocrinol., April 1, 2005; 19(4): 913 - 924. [Abstract] [Full Text] [PDF]

				S. Schinner, A. Barthel, C. Dellas, R. Grzeskowiak, S. K. Sharma, E. Oetjen, R. Blume, and W. Knepel Protein Kinase B Activity Is Sufficient to Mimic the Effect of Insulin on Glucagon Gene Transcription J. Biol. Chem., February 25, 2005; 280(8): 7369 - 7376. [Abstract] [Full Text] [PDF]

				M. E. Scassa, A. S. Guberman, J. M. Ceruti, and E. T. Canepa Hepatic Nuclear Factor 3 and Nuclear Factor 1 Regulate 5-Aminolevulinate Synthase Gene Expression and Are Involved in Insulin Repression J. Biol. Chem., July 2, 2004; 279(27): 28082 - 28092. [Abstract] [Full Text] [PDF]

				H. S. Sun, K.-Y. Hsiao, C.-C. Hsu, M.-H. Wu, and S.-J. Tsai Transactivation of Steroidogenic Acute Regulatory Protein in Human Endometriotic Stromal Cells Is Mediated by the Prostaglandin EP2 Receptor Endocrinology, September 1, 2003; 144(9): 3934 - 3942. [Abstract] [Full Text] [PDF]

				M. Waltner-Law, D. T. Duong, M. C. Daniels, B. Herzog, X. L. Wang, R. Prasad, and D. K. Granner Elements of the Glucocorticoid and Retinoic Acid Response Units Are Involved in cAMP-mediated Expression of the PEPCK Gene J. Biol. Chem., March 14, 2003; 278(12): 10427 - 10435. [Abstract] [Full Text] [PDF]

				C. Anderwald, G. Muller, G. Koca, C. Furnsinn, W. Waldhausl, and M. Roden Short-Term Leptin-Dependent Inhibition of Hepatic Gluconeogenesis Is Mediated by Insulin Receptor Substrate-2 Mol. Endocrinol., July 1, 2002; 16(7): 1612 - 1628. [Abstract] [Full Text] [PDF]

				S. Yang, Z. Fang, T. Suzuki, H. Sasano, J. Zhou, B. Gurates, M. Tamura, K. Ferrer, and S. Bulun Regulation of Aromatase P450 Expression in Endometriotic and Endometrial Stromal Cells by CCAAT/Enhancer Binding Proteins (C/EBPs): Decreased C/EBP{beta} in Endometriosis Is Associated with Overexpression of Aromatase J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2336 - 2345. [Abstract] [Full Text] [PDF]

				E. A. Felinski and P. G. Quinn The coactivator dTAFII110/hTAFII135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB PNAS, October 25, 2001; (2001) 241337698. [Abstract] [Full Text] [PDF]

				P. A. Lochhead, M. Coghlan, S. Q.J. Rice, and C. Sutherland Inhibition of GSK-3 Selectively Reduces Glucose-6-Phosphatase and Phosphoenolpyruvate Carboxykinase Gene Expression Diabetes, May 1, 2001; 50(5): 937 - 946. [Abstract] [Full Text]

				J.-a Kim, J. Lu, and P. G. Quinn Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation PNAS, October 10, 2000; 97(21): 11292 - 11296. [Abstract] [Full Text] [PDF]

				J. K. Wickenheisser, P. G. Quinn, V. L. Nelson, R. S. Legro, J. F. Strauss III, and J. M. McAllister. Differential Activity of the Cytochrome P450 17{alpha}-Hydroxylase and Steroidogenic Acute Regulatory Protein Gene Promoters in Normal and Polycystic Ovary Syndrome Theca Cells J. Clin. Endocrinol. Metab., June 1, 2000; 85(6): 2304 - 2311. [Abstract] [Full Text]

				S. M. Crosson and W. J. Roesler Hormonal Regulation of the Phosphoenolpyruvate Carboxykinase Gene. ROLE OF SPECIFIC CCAAT/ENHANCER-BINDING PROTEIN ISOFORMS J. Biol. Chem., February 25, 2000; 275(8): 5804 - 5809. [Abstract] [Full Text] [PDF]

				M. Viljoen, H. Volschenk, R. A. Young, and H. J. J. van Vuuren Transcriptional Regulation of the Schizosaccharomyces pombe Malic Enzyme Gene, mae2 J. Biol. Chem., April 9, 1999; 274(15): 9969 - 9975. [Abstract] [Full Text] [PDF]

				J. M. Agati, D. Yeagley, and P. G. Quinn Assessment of the Roles of Mitogen-activated Protein Kinase, Phosphatidylinositol 3-Kinase, Protein Kinase B, and Protein Kinase C in Insulin Inhibition of cAMP-induced Phosphoenolpyruvate Carboxykinase Gene Transcription J. Biol. Chem., July 24, 1998; 273(30): 18751 - 18759. [Abstract] [Full Text] [PDF]

				R. Grzeskowiak, J. Amin, E. Oetjen, and W. Knepel Insulin Responsiveness of the Glucagon Gene Conferred by Interactions between Proximal Promoter and More Distal Enhancer-like Elements Involving the Paired-domain Transcription Factor Pax6 J. Biol. Chem., September 22, 2000; 275(39): 30037 - 30045. [Abstract] [Full Text] [PDF]

				D. Schmoll, K. S. Walker, D. R. Alessi, R. Grempler, A. Burchell, S. Guo, R. Walther, and T. G. Unterman Regulation of Glucose-6-phosphatase Gene Expression by Protein Kinase Balpha and the Forkhead Transcription Factor FKHR. EVIDENCE FOR INSULIN RESPONSE UNIT-DEPENDENT AND -INDEPENDENT EFFECTS OF INSULIN ON PROMOTER ACTIVITY J. Biol. Chem., November 10, 2000; 275(46): 36324 - 36333. [Abstract] [Full Text] [PDF]

				S. Prosch, A.-K. Heine, H.-D. Volk, and D. H. Kruger CCAAT/Enhancer-binding Proteins alpha and beta Negatively Influence the Capacity of Tumor Necrosis Factor alpha to Up-regulate the Human Cytomegalovirus IE1/2 Enhancer/Promoter by Nuclear Factor kappa B during Monocyte Differentiation J. Biol. Chem., October 26, 2001; 276(44): 40712 - 40720. [Abstract] [Full Text] [PDF]

				D. Yeagley, S. Guo, T. Unterman, and P. G. Quinn Gene- and Activation-specific Mechanisms for Insulin Inhibition of Basal and Glucocorticoid-induced Insulin-like Growth Factor Binding Protein-1 and Phosphoenolpyruvate Carboxykinase Transcription. ROLES OF FORKHEAD AND INSULIN RESPONSE SEQUENCES J. Biol. Chem., August 31, 2001; 276(36): 33705 - 33710. [Abstract] [Full Text] [PDF]

				D. Yeagley, J. Moll, C. A. Vinson, and P. G. Quinn Characterization of Elements Mediating Regulation of Phosphoenolpyruvate Carboxykinase Gene Transcription by Protein Kinase A and Insulin. IDENTIFICATION OF A DISTINCT COMPLEX FORMED IN CELLS THAT MEDIATE INSULIN INHIBITION J. Biol. Chem., June 2, 2000; 275(23): 17814 - 17820. [Abstract] [Full Text] [PDF]

				E. A. Felinski and P. G. Quinn The coactivator dTAFII110/hTAFII135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB PNAS, November 6, 2001; 98(23): 13078 - 13083. [Abstract] [Full Text] [PDF]