HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bemark, M. Articles by Leanderson, T. Search for Related Content PubMed PubMed Citation Articles by Bemark, M. Articles by Leanderson, T. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 30, 18881-18890, July 24, 1998

Purification and Characterization of a Protein Binding to the SP6  Promoter
A POTENTIAL ROLE FOR CArG-BOX BINDING FACTOR-A IN  TRANSCRIPTION

Mats Bemark, Henric Olsson^§, Dick Heinegård^§, and Tomas Leanderson

From the  Immunology and ^§ Connective Tissue Biology Groups, Department of Cellular and Molecular Biology, Lund University, S-220 07 Lund, Sweden

A protein interacting with an A-T-rich region that is a positive control element within the SP6  promoter was purified and identified as CArG-box binding factor-A. The purified protein was shown to interact specifically with the coding strand of single-stranded DNA and, with lower affinity, with double-stranded DNA. A mutation that inhibited binding of the protein to the A-T-rich region also aborted the transcriptional stimulatory effect of the region. Two Ets proteins, PU.1 and elf-1, that have previously been shown to bind to an adjacent DNA element were shown to physically interact with CArG-box binding factor-A. An antiserum raised against the protein recognized two different forms indicating either that different splice-forms of CArG-box binding factor-A are expressed, or that the protein is subject to post-translational modification.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C. S. Raju, C. Goritz, Y. Nord, O. Hermanson, C. Lopez-Iglesias, N. Visa, G. Castelo-Branco, and P. Percipalle In Cultured Oligodendrocytes the A/B-type hnRNP CBF-A Accompanies MBP mRNA Bound to mRNA Trafficking Sequences Mol. Biol. Cell, July 1, 2008; 19(7): 3008 - 3019. [Abstract] [Full Text] [PDF]

				A. Inoue, T. Watanabe, K. Tominaga, K. Tsugawa, K. Nishio, K. P. Takahashi, and K. Kaneda Association of hnRNP S1 proteins with vimentin intermediate filaments in migrating cells J. Cell Sci., May 15, 2005; 118(10): 2303 - 2311. [Abstract] [Full Text] [PDF]

				C. Murgatroyd, A. Wigger, E. Frank, N. Singewald, M. Bunck, F. Holsboer, R. Landgraf, and D. Spengler Impaired Repression at a Vasopressin Promoter Polymorphism Underlies Overexpression of Vasopressin in a Rat Model of Trait Anxiety J. Neurosci., September 1, 2004; 24(35): 7762 - 7770. [Abstract] [Full Text] [PDF]

				E. Boopathi, N. Lenka, S. K. Prabu, J.-K. Fang, F. Wilkinson, M. Atchison, A. Giallongo, and N. G. Avadhani Regulation of Murine Cytochrome c Oxidase Vb Gene Expression during Myogenesis: YY-1 AND HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D-LIKE PROTEIN (JKTBP1) RECIPROCALLY REGULATE TRANSCRIPTION ACTIVITY BY PHYSICAL INTERACTION WITH THE BERF-1/ZBP-89 FACTOR J. Biol. Chem., August 20, 2004; 279(34): 35242 - 35254. [Abstract] [Full Text] [PDF]

				S. Khateb, P. Weisman-Shomer, I. Hershco, L. A. Loeb, and M. Fry Destabilization of tetraplex structures of the fragile X repeat sequence (CGG)n is mediated by homolog-conserved domains in three members of the hnRNP family Nucleic Acids Res., August 9, 2004; 32(14): 4145 - 4154. [Abstract] [Full Text] [PDF]

				S. T. Shi, G.-Y. Yu, and M. M. C. Lai Multiple Type A/B Heterogeneous Nuclear Ribonucleoproteins (hnRNPs) Can Replace hnRNP A1 in Mouse Hepatitis Virus RNA Synthesis J. Virol., October 1, 2003; 77(19): 10584 - 10593. [Abstract] [Full Text] [PDF]

				A. Umar, T. M. Luider, C. A. Berrevoets, J. A. Grootegoed, and A. O. Brinkmann Proteomic Analysis of Androgen-Regulated Protein Expression in a Mouse Fetal Vas Deferens Cell Line Endocrinology, April 1, 2003; 144(4): 1147 - 1154. [Abstract] [Full Text] [PDF]

				P. Weisman-Shomer, E. Cohen, and M. Fry Distinct domains in the CArG-box binding factor A destabilize tetraplex forms of the fragile X expanded sequence d(CGG)n Nucleic Acids Res., September 1, 2002; 30(17): 3672 - 3681. [Abstract] [Full Text] [PDF]

				P. Percipalle, A. Jonsson, D. Nashchekin, C. Karlsson, T. Bergman, A. Guialis, and B. Daneholt Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins Nucleic Acids Res., April 15, 2002; 30(8): 1725 - 1734. [Abstract] [Full Text] [PDF]

				A. M. Mikheev, S. A. Mikheev, Y. Zhang, R. Aebersold, and H. Zarbl CArG binding factor A (CBF-A) is involved in transcriptional regulation of the rat Ha-ras promoter Nucleic Acids Res., October 1, 2000; 28(19): 3762 - 3770. [Abstract] [Full Text] [PDF]

				C.-H. Sun and J.-H. Tai Identification and Characterization of a ran Gene Promoter in the Protozoan Pathogen Giardia lamblia J. Biol. Chem., July 9, 1999; 274(28): 19699 - 19706. [Abstract] [Full Text] [PDF]