HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, S. Articles by Huang, C.-h. Search for Related Content PubMed PubMed Citation Articles by Li, S. Articles by Huang, C.-h. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 30, 19009-19018, July 24, 1998

Calorimetric Studies of Phosphatidylethanolamines with Saturated sn-1 and Dienoic sn-2 Acyl Chains

From the Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908

We have semi-synthesized 18 species of mixed chain phosphatidylethanolamines (PE) in which the sn-1 acyl chain is derived from stearic, arachidic, and behenic acids, and the sn-2 acyl chain is originated from cis,cis-octadecadienoic and cis,cis-eicosadienoic acids with the two methylene-interrupted double bonds located at various positions. These PEs constituting the bilayers in the aqueous dispersion were subjected to differential scanning calorimetric experiments. The T_m values associated with the gel-to-liquid crystalline phase transitions for these PEs are found to be significantly smaller than those of the saturated counterparts. Moreover, the magnitude of the T_m-lowering effect of acyl chain diunsaturation depends critically on the positions of the two methylene-interrupted cis double bonds in the sn-2 acyl chain. Specifically, if the sn-2 acyl chain is derived from cis,cis-octadecadienoic acid, the T_m-lowering effect has the following decreasing order: ^9,12 > ^6,9 > ^12,15. For cis,cis-eicosadienoyl acyl chain, the T_m-lowering effect is stronger in the order of ^10,13 > ^11,14 > ^8,11 > ^5,8 > ^14,17. Finally, a refined molecular model is presented that can adequately explain the T_m-lowering effect of sn-2 acyl chain diunsaturation. Moreover, this same refined molecular model can also be invoked to better interpret the T_m-lowering effect observed for sn-1 saturated/sn-2 monoenoic PE.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. Serra, B. Pina, J. L. Abad, F. Camps, and G. Fabrias A multifunctional desaturase involved in the biosynthesis of the processionary moth sex pheromone PNAS, October 16, 2007; 104(42): 16444 - 16449. [Abstract] [Full Text] [PDF]

				G. Wang, S. Li, H. Lin, E. E. Brumbaugh, and C.-h. Huang Effects of Various Numbers and Positions of cis Double Bonds in the sn-2 Acyl Chain of Phosphatidylethanolamine on the Chain-melting Temperature J. Biol. Chem., April 30, 1999; 274(18): 12289 - 12299. [Abstract] [Full Text] [PDF]