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J Biol Chem, Vol. 273, Issue 30, 19055-19059, July 24, 1998
5-Fatty Acid Desaturase Gene from
Mortierella alpina
,,
, and
From the Arachidonic acid (C20:4
School of Biological Sciences, Institute of
Arable Crops Research-Long Ashton Research Station,
5,8,11,14) is a polyunsaturated fatty acid
synthesized by the 5-fatty acid desaturation of
di-homo-
-linolenic acid (C20:3 8,11,14). In mammals,
it is known to be a precursor of the prostaglandins and the
leukotrienes but it is also accumulated by the filamentous fungus
Mortierella alpina. We have isolated a cDNA encoding
the 5-fatty acid desaturase from M. alpina
via a polymerase chain reaction-based strategy using primers designed
to the conserved histidine box regions of microsomal desaturases, and
confirmed its function by expression in the yeast Saccharomyces
cerevisiae. Analysis of the lipids from the transformed yeast
demonstrated the accumulation of arachidonic acid. The M. alpina 5-desaturase is the first example of a
cloned 5-desaturase, and differs from other fungal
desaturases previously characterized by the presence of an N-terminal
domain related to cytochrome b5.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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