JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Phelan, S. A.
Right arrow Articles by Loeken, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Phelan, S. A.
Right arrow Articles by Loeken, M. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 30, 19153-19159, July 24, 1998

Identification of a New Binding Motif for the Paired Domain of Pax-3 and Unusual Characteristics of Spacing of Bipartite Recognition Elements on Binding and Transcription Activation

Shelley A. PhelanDagger § and Mary R. LoekenDagger §parallel

From the Dagger  Section on Molecular Biology, Joslin Diabetes Center, Boston, Massachusetts 02215 and the § Division of Medical Sciences and the parallel  Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115

Pax-3, a transcription factor that is required for development of the embryonic neural tube, neural crest, and somitic derivatives, contains two DNA-binding domains, a paired domain, and a paired-type homeodomain. Although Pax-3 binds to sequences related to the e5 element of the Drosophila even-skipped gene, the sequence requirements of an optimal Pax-3 response element have not been well characterized. Using both DNA-binding domains and a pool of random oligonucleotides, we identified a new paired box consensus motif, "GTTAT," which was located 1, 4, 5, 8, or 13 base pairs downstream of the homeobox binding motif, "ATTA." Binding analysis of these sequences demonstrated that the distance between recognition elements for the homeodomain and the paired domain affects affinity. Specifically, spacing elements 1 or 13 base pairs apart from each other conferred low affinity Pax-3 binding, whereas intermediate spacing (5 or 8 base pairs) conferred high affinity binding. Contrary to previous reports, oligonucleotides deleted for either the ATTA or the GTTAT could also be bound by Pax-3, although both sites were necessary for maximal affinity. Finally, transient transfections demonstrated that Pax-3 trans-activation correlated with binding affinity. Because the Pax-3-responsive genes identified to date contain almost exclusively low affinity binding sequences, our analysis indicates that they may be responsive to Pax-3 only when cellular levels are high.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
R. B. White and M. R. Ziman
Genome-wide discovery of Pax7 target genes during development
Physiol Genomics, October 8, 2008; 33(1): 41 - 49.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. S. K. Mayanil, A. Pool, H. Nakazaki, A. C. Reddy, B. Mania-Farnell, B. Yun, D. George, D. G. McLone, and E. G. Bremer
Regulation of Murine TGFbeta2 by Pax3 during Early Embryonic Development
J. Biol. Chem., August 25, 2006; 281(34): 24544 - 24552.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. Li, M. Chase, S.-K. Jung, P. J. S. Smith, and M. R. Loeken
Hypoxic stress in diabetic pregnancy contributes to impaired embryo gene expression and defective development by inducing oxidative stress
Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E591 - E599.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Armoni, M. J. Quon, G. Maor, S. Avigad, D. N. Shapiro, C. Harel, D. Esposito, Y. Goshen, I. Yaniv, and E. Karnieli
PAX3/Forkhead Homolog in Rhabdomyosarcoma Oncoprotein Activates Glucose Transporter 4 Gene Expression in Vivo and in Vitro
J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 5312 - 5324.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
L. Pani, M. Horal, and M. R. Loeken
Rescue of neural tube defects in Pax-3-deficient embryos by p53 loss of function: implications for Pax-3- dependent development and tumorigenesis
Genes & Dev., March 15, 2002; 16(6): 676 - 680.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. C. Chang, L. A. Penoyer, and K. J. Kwon-Chung
The second STE12 homologue of Cryptococcus neoformans is MATa-specific and plays an important role in virulence
PNAS, February 22, 2001; (2001) 61031998.
[Abstract] [Full Text]

Home page
 Hum Mol GenetHome page
N. Bondurand, V. Pingault, D. E. Goerich, N. Lemort, E. Sock, C. L. Caignec, M. Wegner, and M. Goossens
Interaction among SOX10, PAX3 and MITF, three genes altered in Waardenburg syndrome
Hum. Mol. Genet., August 12, 2000; 9(13): 1907 - 1917.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. J. Fredericks, K. Ayyanathan, M. Herlyn, J. R. Friedman, and F. J. Rauscher III
An Engineered PAX3-KRAB Transcriptional Repressor Inhibits the Malignant Phenotype of Alveolar Rhabdomyosarcoma Cells Harboring the Endogenous PAX3-FKHR Oncogene
Mol. Cell. Biol., July 15, 2000; 20(14): 5019 - 5031.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
S. B. Smith, H. C. Ee, J. R. Conners, and M. S. German
Paired-Homeodomain Transcription Factor PAX4 Acts as a Transcriptional Repressor in Early Pancreatic Development
Mol. Cell. Biol., December 1, 1999; 19(12): 8272 - 8280.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Fujitani, Y. Kajimoto, T. Yasuda, T.-A. Matsuoka, H. Kaneto, Y. Umayahara, N. Fujita, H. Watada, J.-I. Miyazaki, Y. Yamasaki, et al.
Identification of a Portable Repression Domain and an E1A-Responsive Activation Domain in Pax4: a Possible Role of Pax4 as a Transcriptional Repressor in the Pancreas
Mol. Cell. Biol., December 1, 1999; 19(12): 8281 - 8291.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. S. K. Mayanil, D. George, B. Mania-Farnell, C. L. Bremer, D. G. McLone, and E. G. Bremer
Overexpression of Murine Pax3 Increases NCAM Polysialylation in a Human Medulloblastoma Cell Line
J. Biol. Chem., July 21, 2000; 275(30): 23259 - 23266.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. S. K. Mayanil, D. George, L. Freilich, E. J. Miljan, B. Mania-Farnell, D. G. McLone, and E. G. Bremer
Microarray Analysis Detects Novel Pax3 Downstream Target Genes
J. Biol. Chem., December 21, 2001; 276(52): 49299 - 49309.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. C. Chang, L. A. Penoyer, and K. J. Kwon-Chung
The second STE12 homologue of Cryptococcus neoformans is MATa-specific and plays an important role in virulence
PNAS, March 13, 2001; 98(6): 3258 - 3263.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.