JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An addition or correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, K.-K.
Right arrow Articles by Yonehara, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, K.-K.
Right arrow Articles by Yonehara, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 30, 19160-19166, July 24, 1998

Purification, Molecular Cloning, and Characterization of TRP32, a Novel Thioredoxin-related Mammalian Protein of 32 kDa

Kyung-Kwon LeeDagger , Masao MurakawaDagger , Shu TakahashiDagger , Satoshi Tsubuki§, Sei-ichi Kawashima§, Kazuhiro SakamakiDagger , and Shin YoneharaDagger

From the Dagger  Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan and the § Tokyo Metropolitan Institute of Medical Science, Tokyo 113-0021, Japan

We purified a protein of 32 kDa from human thymoma HPB-ALL cells that was co-purified with a catalytic fragment of MST (mammalian STE-20-like), a kinase of the STE20 family, which is proteolytically activated by caspase in apoptosis (Lee, K.-K., Murakawa, M., Nishida, E., Tsubuki, S., Kawashima, S., Sakamaki, K., and Yonehara, S. (1998) Oncogene 16, in press). Molecular cloning of the gene encoding this 32-kDa protein (TRP32) reveals that it is a novel protein of 289 amino acid residues and contains an NH2-terminal thioredoxin domain with a conserved thioredoxin active site. The human and mouse TRP32 proteins have 99% homology, and the thioredoxin domains are completely identical. The thioredoxin domain of TRP32 has thioredoxin-like reducing activity, which can reduce the interchain disulfide bridges of insulin in vitro. However, the thioredoxin domain of TRP32 is more sensitive to oxidation than human thioredoxin. Northern blot analysis showed that TRP32 is expressed in all human tissues. Expression of TRP32 was also confirmed in all mammalian cell lines tested by Western blot analysis using anti-TRP32 monoclonal antibody. Subcellular fractionation and immunostaining analysis showed TRP32 is cytoplasmic protein. These findings suggest that TRP32 is a novel cytoplasmic regulator of the redox state in higher eukaryotes.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. Moriuchi, N. Koda, E. Okuda-Ashitaka, H. Daiyasu, K. Ogasawara, H. Toh, S. Ito, D. F. Woodward, and K. Watanabe
Molecular Characterization of a Novel Type of Prostamide/Prostaglandin F Synthase, Belonging to the Thioredoxin-like Superfamily
J. Biol. Chem., January 11, 2008; 283(2): 792 - 801.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W. D'Hertog, L. Overbergh, K. Lage, G. B. Ferreira, M. Maris, C. Gysemans, D. Flamez, A. K. Cardozo, G. Van den Bergh, L. Schoofs, et al.
Proteomics Analysis of Cytokine-induced Dysfunction and Death in Insulin-producing INS-1E Cells: New Insights into the Pathways Involved
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2180 - 2199.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
F. Malagnac, B. Klapholz, and P. Silar
PaTrx1 and PaTrx3, Two Cytosolic Thioredoxins of the Filamentous Ascomycete Podospora anserina Involved in Sexual Development and Cell Degeneration
Eukaryot. Cell, December 1, 2007; 6(12): 2323 - 2331.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Berndt, C. H. Lillig, and A. Holmgren
Thiol-based mechanisms of the thioredoxin and glutaredoxin systems: implications for diseases in the cardiovascular system
Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1227 - H1236.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
J. Song, R. C. Tyler, R. L. Wrobel, R. O. Frederick, F. C. Vojtek, W. B. Jeon, M. S. Lee, and J. L. Markley
Solution structure of At3g04780.1-des15, an Arabidopsis thaliana ortholog of the C-terminal domain of human thioredoxin-like protein
Protein Sci., April 1, 2005; 14(4): 1059 - 1063.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wang, X. De Deken, M. Milenkovic, Y. Song, I. Pirson, J. E. Dumont, and F. Miot
Identification of a Novel Partner of Duox: EFP1, A THIOREDOXIN-RELATED PROTEIN
J. Biol. Chem., January 28, 2005; 280(4): 3096 - 3103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. R. Woo, S. J. Kim, W. Jeong, Y. H. Cho, S. C. Lee, Y. J. Chung, S. G. Rhee, and S. E. Ryu
Structural Basis of Cellular Redox Regulation by Human TRP14
J. Biol. Chem., November 12, 2004; 279(46): 48120 - 48125.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Jimenez, W. Zu, V. Y. Rawe, M. Pelto-Huikko, C. J. Flickinger, P. Sutovsky, J.-A. Gustafsson, R. Oko, and A. Miranda-Vizuete
Spermatocyte/Spermatid-specific Thioredoxin-3, a Novel Golgi Apparatus-associated Thioredoxin, Is a Specific Marker of Aberrant Spermatogenesis
J. Biol. Chem., August 13, 2004; 279(33): 34971 - 34982.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
W. H. Watson, X. Yang, Y. E. Choi, D. P. Jones, and J. P. Kehrer
Thioredoxin and Its Role in Toxicology
Toxicol. Sci., March 1, 2004; 78(1): 3 - 14.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Jeong, H. W. Yoon, S.-R. Lee, and S. G. Rhee
Identification and Characterization of TRP14, a Thioredoxin-related Protein of 14 kDa: NEW INSIGHTS INTO THE SPECIFICITY OF THIOREDOXIN FUNCTION
J. Biol. Chem., January 30, 2004; 279(5): 3142 - 3150.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. H. LECKER, R. T. JAGOE, A. GILBERT, M. GOMES, V. BARACOS, J. BAILEY, S. R. PRICE, W. E. MITCH, and A. L. GOLDBERG
Multiple types of skeletal muscle atrophy involve a common program of changes in gene expression
FASEB J, January 1, 2004; 18(1): 39 - 51.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Miranda-Vizuete, K. Tsang, Y. Yu, A. Jimenez, M. Pelto-Huikko, C. J. Flickinger, P. Sutovsky, and R. Oko
Cloning and Developmental Analysis of Murid Spermatid-specific Thioredoxin-2 (SPTRX-2), a Novel Sperm Fibrous Sheath Protein and Autoantigen
J. Biol. Chem., November 7, 2003; 278(45): 44874 - 44885.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. K. Tarka-Leeds, J. D. Suarez, N. L. Roberts, J. M. Rogers, M. P. Hardy, and G. R. Klinefelter
Gestational Exposure to Ethane Dimethanesulfonate Permanently Alters Reproductive Competence in the CD-1 Mouse
Biol Reprod, September 1, 2003; 69(3): 959 - 967.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Nguyen, J.-L. Teo, A. Matsuda, M. Eguchi, E. Y. Chi, W. R. Henderson Jr., and M. Kahn
Chemogenomic identification of Ref-1/AP-1 as a therapeutic target for asthma
PNAS, February 4, 2003; 100(3): 1169 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-K. Lee and S. Yonehara
Phosphorylation and Dimerization Regulate Nucleocytoplasmic Shuttling of Mammalian STE20-like Kinase (MST)
J. Biol. Chem., March 29, 2002; 277(14): 12351 - 12358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. A. Reszka, J. M. Halasy-Nagy, P. J. Masarachia, and G. A. Rodan
Bisphosphonates Act Directly on the Osteoclast to Induce Caspase Cleavage of Mst1 Kinase during Apoptosis. A LINK BETWEEN INHIBITION OF THE MEVALONATE PATHWAY AND REGULATION OF AN APOPTOSIS-PROMOTING KINASE
J. Biol. Chem., December 3, 1999; 274(49): 34967 - 34973.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-K. Lee, T. Ohyama, N. Yajima, S. Tsubuki, and S. Yonehara
MST, a Physiological Caspase Substrate, Highly Sensitizes Apoptosis Both Upstream and Downstream of Caspase Activation
J. Biol. Chem., May 25, 2001; 276(22): 19276 - 19285.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Matsuo, N. Akiyama, H. Nakamura, J. Yodoi, M. Noda, and S. Kizaka-Kondoh
Identification of a Novel Thioredoxin-related Transmembrane Protein
J. Biol. Chem., March 23, 2001; 276(13): 10032 - 10038.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. N. Gladyshev, A. Liu, S. V. Novoselov, K. Krysan, Q.-A. Sun, V. M. Kryukov, G. V. Kryukov, and M. F. Lou
Identification and Characterization of a New Mammalian Glutaredoxin (Thioltransferase), Grx2
J. Biol. Chem., August 3, 2001; 276(32): 30374 - 30380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Ma, S. Karra, W. Guo, D. J. Lindner, J. Hu, J. E. Angell, E. R. Hofmann, S. P. M. Reddy, and D. V. Kalvakolanu
Regulation of Interferon and Retinoic Acid-induced Cell Death Activation through Thioredoxin Reductase
J. Biol. Chem., June 29, 2001; 276(27): 24843 - 24854.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.