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J Biol Chem, Vol. 273, Issue 30, 19213-19219, July 24, 1998
Protein Kinase C Regulates Chondrogenesis of Mesenchymes via
Mitogen-activated Protein Kinase Signaling
Sung-Hee
Chang ,
Chun-Do
Oh ,
Myung-Soon
Yang ,
Shin-Sung
Kang ,
Young-Sup
Lee§,
Jong-Kyung
Sonn¶, and
Jang-Soo
Chun
From the Department of Biology and
§ Biochemistry, College of Natural Sciences and
¶ Teacher's College, Kyungpook National University,
Taegu 702-701, Korea
A possible regulatory mechanism of protein kinase
C (PKC) in the chondrogenesis of chick limb bud mesenchymes has been
investigated. Inhibition or down-regulation of PKC resulted in the
activation of a mitogen-activated protein kinase subtype Erk-1 and the
inhibition of chondrogenesis. On the other hand, inhibition of Erk-1
with PD98059 enhanced chondrogenesis and relieved PKC-induced blockage of chondrogenesis. Erk-1 inhibition, however, did not affect expression and subcellular distribution of PKC isoforms expressed in mesenchymes nor cell proliferation. The results suggest that PKC regulates chondrogenesis by modulating Erk-1 activity. Inhibition or depletion of
PKC inhibited proliferation of chondrogenic competent cells, and Erk-1
inhibition did not affect PKC modulation of cell proliferation. However, PKC-induced modulation of expression of cell adhesion molecules involved in precartilage condensation was reversed by the
inhibition of Erk-1. Expression of N-cadherin was detected at the early
period of chondrogenesis. Inhibition or depletion of PKC induced
sustained expression of N-cadherin, and Erk-1 inhibition blocked the
effects of PKC modulation. The expression of integrin 5 1 and
fibronectin was found to be increased transiently during chondrogenesis. Depletion or inhibition of PKC caused a continuous increase of the expression of these molecules throughout the culture period, and Erk-1 inhibition abolished the modulating effects of PKC.
Because reduction of the examined cell adhesion molecule expression is
a prerequisite for the progression of chondrogenesis after cell
condensation, our results indicate that PKC regulates chondrogenesis by
modulating expression of these molecules via Erk-1 signaling.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.

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Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.
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