JBC INTERFERin siRNA transfection reagent

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J Biol Chem, Vol. 273, Issue 30, 19235-19242, July 24, 1998

ZmcPKC70, a Protein Kinase C-type Enzyme from Maize
BIOCHEMICAL CHARACTERIZATION, REGULATION BY PHORBOL 12-MYRISTATE 13-ACETATE AND ITS POSSIBLE INVOLVEMENT IN NITRATE REDUCTASE GENE EXPRESSION

Meena R. ChandokDagger and Sudhir K. SoporyDagger

From the Dagger  Centre for Plant Molecular Biology, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India and the  International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India

The crucial enzyme in diacylglycerol-mediated signaling is protein kinase C (PKC). In this paper we provide evidence for the existence and role of PKC in maize. A protein of an apparent molecular mass of 70 kDa was purified. The protein showed kinase activity that was stimulated by phosphatidylserine and oleyl acetyl glycerol (OAG) in the presence of Ca2+. Phorbol 12-myristate 13-acetate (PMA) replaced the requirement of OAG. [3H]PMA binding to the 70-kDa protein was competed by unlabeled PMA and OAG but not by 4alpha -PMA, an inactive analog. The kinase phosphorylates histone H1 at serine residue(s), and this activity was inhibited by H-7 and staurosporine. These properties suggest that the 70-kDa protein is a conventional serine/threonine protein kinase C (cPKC). Polyclonal antibodies raised against the polypeptide precipitate the enzyme activity and immunostained the protein on Western blots. The antibodies also cross-reacted with a protein of expected size from sorghum, rice, and tobacco. A rapid increase in the protein level was observed in maize following PMA treatments. In order to assign a possible role of PKC in gene regulation, the nitrate reductase transcript level was investigated. The transcript level increased by PMA, not by 4alpha -PMA treatments, and the increase was inhibited by H-7 but not by okadaic acid. The data show the existence and possible function of PKC in higher plants.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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