HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, Y. Articles by Chiu, I.-M. Search for Related Content PubMed PubMed Citation Articles by Liu, Y. Articles by Chiu, I.-M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 30, 19269-19276, July 24, 1998

A Splice Variant of E2-2 Basic Helix-Loop-Helix Protein Represses the Brain-specific Fibroblast Growth Factor 1 Promoter through the Binding to an Imperfect E-box

From the Department of Internal Medicine and Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210

We previously demonstrated that a cis-element (489 to 467) in the brain-specific fibroblast growth factor (FGF)-1 promoter (FGF-1.B) binds multiple nuclear factors, and this binding enhances transcriptional activity of this promoter. Here we report the isolation of three cDNA clones, VL1, VL2 and VL3, from a human brain stem cDNA expression library using four tandem repeats of the 26-base pair sequence (492 to 467) as the probe. These cDNA clones represent the variant of bHLH protein E2-2/SEF2-1 in having 12 additional nucleotides encoding the amino acids RSRS. The glutathione S-transferase (GST) fusion proteins of VLl, VL2, and VL3 immunologically react with anti-E2-2 antibody and anti-GST-VL2 antibody. Electrophoretic mobility shift assay and methylation interference assay revealed that the GST fusion proteins specifically bind to an imperfect E-box sequence (GACCTG) present in the 26-base pair sequence. Transient expression of the full-length E2-2 without RSRS in U1240MG glioblastoma cells resulted in repression of FGF-1.B promoter activity. We further showed a significant repression of promoter activity (>40 fold) by E2-2 (lacking the amino acid sequence RSRS) when the E47 reporter construct, containing a hexameric E-box site, was used. In contrast, the E2-2 variant containing the RSRS sequence has no significant effect on either the FGF-1 promoter or E47 promoter. These results suggest that the relative abundance of the two splice variants of E2-2 in brain could be an important determinant for the expression of FGF-1.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C Zweier, H Sticht, E K Bijlsma, J Clayton-Smith, S E Boonen, A Fryer, M T Greally, L Hoffmann, N S den Hollander, M Jongmans, et al. Further delineation of Pitt-Hopkins syndrome: phenotypic and genotypic description of 16 novel patients J. Med. Genet., November 1, 2008; 45(11): 738 - 744. [Abstract] [Full Text] [PDF]

				L. C. Kudo, S. L. Karsten, J. Chen, P. Levitt, and D. H. Geschwind Genetic Analysis of Anterior Posterior Expression Gradients in the Developing Mammalian Forebrain Cereb Cortex, September 1, 2007; 17(9): 2108 - 2122. [Abstract] [Full Text] [PDF]

				A. Brockschmidt, U. Todt, S. Ryu, A. Hoischen, C. Landwehr, S. Birnbaum, W. Frenck, B. Radlwimmer, P. Lichter, H. Engels, et al. Severe mental retardation with breathing abnormalities (Pitt-Hopkins syndrome) is caused by haploinsufficiency of the neuronal bHLH transcription factor TCF4 Hum. Mol. Genet., June 15, 2007; 16(12): 1488 - 1494. [Abstract] [Full Text] [PDF]

				J.-i. Hikima, C. C. Cioffi, D. L. Middleton, M. R. Wilson, N. W. Miller, L. W. Clem, and G. W. Warr Evolution of Transcriptional Control of the IgH Locus: Characterization, Expression, and Function of TF12/HEB Homologs of the Catfish J. Immunol., November 1, 2004; 173(9): 5476 - 5484. [Abstract] [Full Text] [PDF]

				F. Madiai, K. V. Hackshaw, and I.-M. Chiu Characterization of the Entire Transcription Unit of the Mouse Fibroblast Growth Factor 1 (FGF-1) Gene. TISSUE-SPECIFIC EXPRESSION OF THE FGF-1.A mRNA J. Biol. Chem., April 23, 1999; 274(17): 11937 - 11944. [Abstract] [Full Text] [PDF]

				M. A. Chotani, K. Touhalisky, and I.-M. Chiu The Small GTPases Ras, Rac, and Cdc42 Transcriptionally Regulate Expression of Human Fibroblast Growth Factor 1 J. Biol. Chem., September 22, 2000; 275(39): 30432 - 30438. [Abstract] [Full Text] [PDF]

				M. Furumura, S. B. Potterf, K. Toyofuku, J. Matsunaga, J. Muller, and V. J. Hearing Involvement of ITF2 in the Transcriptional Regulation of Melanogenic Genes J. Biol. Chem., July 20, 2001; 276(30): 28147 - 28154. [Abstract] [Full Text] [PDF]