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J Biol Chem, Vol. 273, Issue 30, 19269-19276, July 24, 1998
From the Department of Internal Medicine and Comprehensive Cancer
Center, Ohio State University, Columbus, Ohio 43210
We previously demonstrated that a
cis-element (
A Splice Variant of E2-2 Basic Helix-Loop-Helix Protein
Represses the Brain-specific Fibroblast Growth Factor 1 Promoter
through the Binding to an Imperfect E-box
489 to
467) in the brain-specific
fibroblast growth factor (FGF)-1 promoter (FGF-1.B) binds multiple
nuclear factors, and this binding enhances transcriptional activity of
this promoter. Here we report the isolation of three cDNA clones,
VL1, VL2 and VL3, from a human brain stem cDNA expression library
using four tandem repeats of the 26-base pair sequence (
492 to
467)
as the probe. These cDNA clones represent the variant of bHLH
protein E2-2/SEF2-1 in having 12 additional nucleotides encoding the
amino acids RSRS. The glutathione S-transferase (GST)
fusion proteins of VLl, VL2, and VL3 immunologically react with
anti-E2-2 antibody and anti-GST-VL2 antibody. Electrophoretic mobility
shift assay and methylation interference assay revealed that the GST
fusion proteins specifically bind to an imperfect E-box sequence
(GACCTG) present in the 26-base pair sequence. Transient expression of
the full-length E2-2 without RSRS in U1240MG glioblastoma cells
resulted in repression of FGF-1.B promoter activity. We further showed
a significant repression of promoter activity (>40 fold) by E2-2
(lacking the amino acid sequence RSRS) when the E47 reporter construct,
containing a hexameric E-box site, was used. In contrast, the E2-2
variant containing the RSRS sequence has no significant effect on
either the FGF-1 promoter or E47 promoter. These results suggest that
the relative abundance of the two splice variants of E2-2 in brain
could be an important determinant for the expression of FGF-1.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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