HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hii, C. S. T. Articles by Ferrante, A. Search for Related Content PubMed PubMed Citation Articles by Hii, C. S. T. Articles by Ferrante, A. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 30, 19277-19282, July 24, 1998

Stimulation of p38 Phosphorylation and Activity by Arachidonic Acid in HeLa Cells, HL60 Promyelocytic Leukemic Cells, and Human Neutrophils
EVIDENCE FOR CELL TYPE-SPECIFIC ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES

Charles S. T. Hii, Zhi H. Huang, Andrea Bilney^¶, Maurizio Costabile, Andrew W. Murray^¶, Deborah A. Rathjen, Channing J Der, and Antonio Ferrante

From the  Department of Immunopathology, Women's and Children's Hospital, North Adelaide, South Australia 5006 and ^¶ School of Biological Sciences, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia and  Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599

Although it is well appreciated that arachidonic acid, a second messenger molecule that is released by ligand-stimulated phospholipase A₂, stimulates a wide range of cell types, the mechanisms that mediate the actions of arachidonic acid are still poorly understood. We now report that arachidonic acid stimulated the appearance of dual-phosphorylated (active) p38 mitogen-activated protein kinase as detected by Western blotting in HeLa cells, HL60 cells, human neutrophils, and human umbilical vein endothelial cells but not Jurkat cells. An increase in p38 kinase activity caused by arachidonic acid was also observed. Further studies with neutrophils show that the stimulation of p38 dual phosphorylation by arachidonic acid was transient, peaking at 5 min, and was concentration-dependent. The effect of arachidonic acid was not affected by either nordihydroguaiaretic acid, an inhibitor of the 5-, 12-, and 15-lipoxygenases or by indomethacin, an inhibitor of cyclooxygenase. Arachidonic acid also stimulated the phosphorylation and/or activity of the extracellular signal-regulated protein kinase and of c-jun N-terminal kinase in a cell-type-specific manner. An examination of the mechanisms through which arachidonic acid stimulated the phosphorylation/activity of p38 and extracellular signal-regulated protein kinase in neutrophils revealed an involvement of protein kinase C. Thus, arachidonic acid stimulated the translocation of protein kinase C , I, and II to a particulate fraction, and the effects of arachidonic acid on mitogen-activated protein kinase phosphorylation/activity were partially inhibited by GF109203X, an inhibitor of protein kinase C. This study is the first to demonstrate that a polyunsaturated fatty acid causes the dual phosphorylation and activation of p38.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C. Marantos, V. Mukaro, J. Ferrante, C. Hii, and A. Ferrante Inhibition of the Lipopolysaccharide-Induced Stimulation of the Members of the MAPK Family in Human Monocytes/Macrophages by 4-Hydroxynonenal, a Product of Oxidized Omega-6 Fatty Acids Am. J. Pathol., October 1, 2008; 173(4): 1057 - 1066. [Abstract] [Full Text] [PDF]

				J. Tan, P. P. Dwivedi, P. Anderson, B. K. Nutchey, P. O'Loughlin, H. A. Morris, B. K. May, A. Ferrante, and C. S. Hii Antineoplastic agents target the 25-hydroxyvitamin D3 24-hydroxylase messenger RNA for degradation: implications in anticancer activity Mol. Cancer Ther., December 1, 2007; 6(12): 3131 - 3138. [Abstract] [Full Text] [PDF]

				D. Morgan, V. V. Cherny, A. Finnegan, J. Bollinger, M. H. Gelb, and T. E. DeCoursey Sustained activation of proton channels and NADPH oxidase in human eosinophils and murine granulocytes requires PKC but not cPLA2{alpha} activity J. Physiol., March 1, 2007; 579(2): 327 - 344. [Abstract] [Full Text] [PDF]

				A. Ferrante, B. S. Robinson, H. Singh, H. P.A. Jersmann, J. V. Ferrante, Z. H. Huang, N. A. Trout, M. J. Pitt, D. A. Rathjen, C. J. Easton, et al. A Novel {beta}-Oxa Polyunsaturated Fatty Acid Downregulates the Activation of the I{kappa}B Kinase/Nuclear Factor {kappa}B Pathway, Inhibits Expression of Endothelial Cell Adhesion Molecules, and Depresses Inflammation Circ. Res., July 7, 2006; 99(1): 34 - 41. [Abstract] [Full Text] [PDF]

				H. Malhi, S. F. Bronk, N. W. Werneburg, and G. J. Gores Free Fatty Acids Induce JNK-dependent Hepatocyte Lipoapoptosis J. Biol. Chem., April 28, 2006; 281(17): 12093 - 12101. [Abstract] [Full Text] [PDF]

				N. Flamand, J. Lefebvre, M. E. Surette, S. Picard, and P. Borgeat Arachidonic Acid Regulates the Translocation of 5-Lipoxygenase to the Nuclear Membranes in Human Neutrophils J. Biol. Chem., January 6, 2006; 281(1): 129 - 136. [Abstract] [Full Text] [PDF]

				C. Kim and M. C. Dinauer Impaired NADPH oxidase activity in Rac2-deficient murine neutrophils does not result from defective translocation of p47phox and p67phox and can be rescued by exogenous arachidonic acid J. Leukoc. Biol., January 1, 2006; 79(1): 223 - 234. [Abstract] [Full Text] [PDF]

				I. Talukdar, W. Szeszel-Fedorowicz, and L. M. Salati Arachidonic Acid Inhibits the Insulin Induction of Glucose-6-phosphate Dehydrogenase via p38 MAP Kinase J. Biol. Chem., December 9, 2005; 280(49): 40660 - 40667. [Abstract] [Full Text] [PDF]

				C. S. Hii, D. S. Anson, M. Costabile, V. Mukaro, K. Dunning, and A. Ferrante Characterization of the MEK5-ERK5 Module in Human Neutrophils and Its Relationship to ERK1/ERK2 in the Chemotactic Response J. Biol. Chem., November 26, 2004; 279(48): 49825 - 49834. [Abstract] [Full Text] [PDF]

				S. J. Feinmark, R. Begum, E. Tsvetkov, I. Goussakov, C. D. Funk, S. A. Siegelbaum, and V. Y. Bolshakov 12-Lipoxygenase Metabolites of Arachidonic Acid Mediate Metabotropic Glutamate Receptor-Dependent Long-Term Depression at Hippocampal CA3-CA1 Synapses J. Neurosci., December 10, 2003; 23(36): 11427 - 11435. [Abstract] [Full Text] [PDF]

				J. Liu, Z. Liu, S. Chuai, and X. Shen Phospholipase C and phosphatidylinositol 3-kinase signaling are involved in the exogenous arachidonic acid-stimulated respiratory burst in human neutrophils J. Leukoc. Biol., September 1, 2003; 74(3): 428 - 437. [Abstract] [Full Text] [PDF]

				N. Moghaddami, M. Costabile, P. K. Grover, H. P. A. Jersmann, Z. H. Huang, C. S. T. Hii, and A. Ferrante Unique Effect of Arachidonic Acid on Human Neutrophil TNF Receptor Expression: Up-Regulation Involving Protein Kinase C, Extracellular Signal-Regulated Kinase, and Phospholipase A2 J. Immunol., September 1, 2003; 171(5): 2616 - 2624. [Abstract] [Full Text] [PDF]

				S. Grenier, N. Flamand, J. Pelletier, P. H. Naccache, P. Borgeat, and S. G. Bourgoin Arachidonic acid activates phospholipase D in human neutrophils; essential role of endogenous leukotriene B4 and inhibition by adenosine A2A receptor engagement J. Leukoc. Biol., April 1, 2003; 73(4): 530 - 539. [Abstract] [Full Text] [PDF]

				D. Wu and A. I. Cederbaum Role of p38 MAPK in CYP2E1-dependent Arachidonic Acid Toxicity J. Biol. Chem., January 3, 2003; 278(2): 1115 - 1124. [Abstract] [Full Text] [PDF]

				E. Burkert, D. Szellas, O. Radmark, D. Steinhilber, and O. Werz Cell type-dependent activation of 5-lipoxygenase by arachidonic acid J. Leukoc. Biol., January 1, 2003; 73(1): 191 - 200. [Abstract] [Full Text] [PDF]

				Y. J. Kim, K. P. Kim, S. K. Han, N. M. Munoz, X. Zhu, H. Sano, A. R. Leff, and W. Cho Group V Phospholipase A2 Induces Leukotriene Biosynthesis in Human Neutrophils through the Activation of Group IVA Phospholipase A2 J. Biol. Chem., September 20, 2002; 277(39): 36479 - 36488. [Abstract] [Full Text] [PDF]

				P. P. Dwivedi, C. S. T. Hii, A. Ferrante, J. Tan, C. J. Der, J. L. Omdahl, H. A. Morris, and B. K. May Role of MAP Kinases in the 1,25-Dihydroxyvitamin D3-induced Transactivation of the Rat Cytochrome P450C24 (CYP24) Promoter. SPECIFIC FUNCTIONS FOR ERK1/ERK2 AND ERK5 J. Biol. Chem., August 9, 2002; 277(33): 29643 - 29653. [Abstract] [Full Text] [PDF]

				D. Cussac, S. Schaak, C. Denis, and H. Paris alpha 2B-Adrenergic Receptor Activates MAPK via a Pathway Involving Arachidonic Acid Metabolism, Matrix Metalloproteinases, and Epidermal Growth Factor Receptor Transactivation J. Biol. Chem., May 24, 2002; 277(22): 19882 - 19888. [Abstract] [Full Text] [PDF]

				O. Werz, D. Szellas, D. Steinhilber, and O. Radmark Arachidonic Acid Promotes Phosphorylation of 5-Lipoxygenase at Ser-271 by MAPK-activated Protein Kinase 2 (MK2) J. Biol. Chem., April 19, 2002; 277(17): 14793 - 14800. [Abstract] [Full Text] [PDF]

				J. P. Walterscheid, S. E. Ullrich, and D. X. Nghiem Platelet-activating Factor, a Molecular Sensor for Cellular Damage, Activates Systemic Immune Suppression J. Exp. Med., January 14, 2002; 195(2): 171 - 179. [Abstract] [Full Text] [PDF]

				M. Costabile, C. S. T. Hii, B. S. Robinson, D. A. Rathjen, M. Pitt, C. Easton, R. C. Miller, A. Poulos, A. W. Murray, and A. Ferrante A Novel Long Chain Polyunsaturated Fatty Acid, {beta}-Oxa 21:3n-3, Inhibits T Lymphocyte Proliferation, Cytokine Production, Delayed-Type Hypersensitivity, and Carrageenan-Induced Paw Reaction and Selectively Targets Intracellular Signals J. Immunol., October 1, 2001; 167(7): 3980 - 3987. [Abstract] [Full Text] [PDF]

				Y. GORIN, N.-H. KIM, D. FELIERS, B. BHANDARI, G. G. CHOUDHURY, and H. E. ABBOUD Angiotensin II activates Akt/protein kinase B by an arachidonic acid/redox-dependent pathway and independent of phosphoinositide 3-kinase FASEB J, September 1, 2001; 15(11): 1909 - 1920. [Abstract] [Full Text] [PDF]

				E. Mollapour, D. C. Linch, and P. J. Roberts Activation and priming of neutrophil nicotinamide adenine dinucleotide phosphate oxidase and phospholipase A2 are dissociated by inhibitors of the kinases p42ERK2 and p38SAPK and by methyl arachidonyl fluorophosphonate, the dual inhibitor of cytosolic and calcium-independent phospholipase A2 Blood, April 15, 2001; 97(8): 2469 - 2477. [Abstract] [Full Text] [PDF]

				L.-C. Chang and J.-P. Wang Signal transduction pathways for activation of extracellular signal-regulated kinase by arachidonic acid in rat neutrophils J. Leukoc. Biol., April 1, 2001; 69(4): 659 - 665. [Abstract] [Full Text]

				K. Mackay and D. Mochly-Rosen Arachidonic acid protects neonatal rat cardiac myocytes from ischaemic injury through {epsilon} protein kinase C Cardiovasc Res, April 1, 2001; 50(1): 65 - 74. [Abstract] [Full Text] [PDF]

				H. P. A. Jersmann, C. S. T. Hii, J. V. Ferrante, and A. Ferrante Bacterial Lipopolysaccharide and Tumor Necrosis Factor Alpha Synergistically Increase Expression of Human Endothelial Adhesion Molecules through Activation of NF-{kappa}B and p38 Mitogen-Activated Protein Kinase Signaling Pathways Infect. Immun., March 1, 2001; 69(3): 1273 - 1279. [Abstract] [Full Text] [PDF]

				E. Paine, R. Palmantier, S. K. Akiyama, K. Olden, and J. D. Roberts Arachidonic Acid Activates Mitogen-activated Protein (MAP) Kinase-activated Protein Kinase 2 and Mediates Adhesion of a Human Breast Carcinoma Cell Line to Collagen Type IV through a p38 MAP Kinase-dependent Pathway J. Biol. Chem., April 6, 2000; 275(15): 11284 - 11290. [Abstract] [Full Text] [PDF]

				Y. Q. Li, C. S. T. Hii, M. Costabile, D. Goh, C. J. Der, and A. Ferrante Regulation of Lymphotoxin Production by the p21ras-raf-MEK-ERK Cascade in PHA/PMA-Stimulated Jurkat Cells J. Immunol., March 15, 1999; 162(6): 3316 - 3320. [Abstract] [Full Text] [PDF]

				C. S. T. Hii, K. Stacey, N. Moghaddami, A. W. Murray, and A. Ferrante Role of the Extracellular Signal-Regulated Protein Kinase Cascade in Human Neutrophil Killing of Staphylococcus aureus and Candida albicans and in Migration Infect. Immun., March 1, 1999; 67(3): 1297 - 1302. [Abstract] [Full Text] [PDF]

				P. Xia, J. R. Gamble, K.-A. Rye, L. Wang, C. S. T. Hii, P. Cockerill, Y. Khew-Goodall, A. G. Bert, P. J. Barter, and M. A. Vadas Tumor necrosis factor-alpha induces adhesion molecule expression through the sphingosine kinase pathway PNAS, November 24, 1998; 95(24): 14196 - 14201. [Abstract] [Full Text] [PDF]

				J. T. O'Flaherty, B. A. Chadwell, M. W. Kearns, S. Sergeant, and L. W. Daniel Protein Kinases C Translocation Responses to Low Concentrations of Arachidonic Acid J. Biol. Chem., June 29, 2001; 276(27): 24743 - 24750. [Abstract] [Full Text] [PDF]

				C. S. T. Hii, N. Moghadammi, A. Dunbar, and A. Ferrante Activation of the Phosphatidylinositol 3-Kinase-Akt/Protein Kinase B Signaling Pathway in Arachidonic Acid-stimulated Human Myeloid and Endothelial Cells. INVOLVEMENT OF THE ErbB RECEPTOR FAMILY J. Biol. Chem., July 13, 2001; 276(29): 27246 - 27255. [Abstract] [Full Text] [PDF]

				J. E. Smolen, T. K. Petersen, C. Koch, S. J. O'Keefe, W. A. Hanlon, S. Seo, D. Pearson, M. C. Fossett, and S. I. Simon L-Selectin Signaling of Neutrophil Adhesion and Degranulation Involves p38 Mitogen-activated Protein Kinase J. Biol. Chem., May 19, 2000; 275(21): 15876 - 15884. [Abstract] [Full Text] [PDF]