JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, X.
Right arrow Articles by Bennett, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, X.
Right arrow Articles by Bennett, V.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 30, 19329-19338, July 24, 1998

Adducin Preferentially Recruits Spectrin to the Fast Growing Ends of Actin Filaments in a Complex Requiring the MARCKS-related Domain and a Newly Defined Oligomerization Domain

Xiaolin Li, Yoichiro Matsuoka, and Vann Bennett

From the Department of Cell Biology and Biochemistry and the Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710

Adducin is a protein associated with spectrin and actin in membrane skeletons of erythrocytes and possibly other cells. Adducin has activities in in vitro assays of association with the sides of actin filaments, capping the fast growing ends of actin filaments, and recruiting spectrin to actin filaments. This study presents evidence that adducin exhibits a preference for the fast growing ends of actin filaments for recruiting spectrin to actin and for direct association with actin. beta -Adducin-(335-726) promoted recruitment of spectrin to gelsolin-sensitive sites at fast growing ends of actin filaments with half-maximal activity at 15 nM and to gelsolin-insensitive sites with half-maximal activity at 75 nM. beta -Adducin-(335-726) also exhibited a preference for actin filament ends in direct binding assays; the half-maximal concentration for binding of adducin to gelsolin-sensitive sites at filament ends was 60 nM, and the Kd for binding to lateral sites was 1.5 µM. The concentration of beta -adducin-(335-726) of 60 nM required for half-maximal binding to filament ends is in the same range as the concentration of 150 nM required for half-maximal actin capping activity. All interactions of adducin with actin require the myristoylated alanine-rich protein kinase C substrate-related domain as well as a newly defined oligomerization site localized in the neck domain of adducin. Surprisingly, the head domain of adducin is not required for spectrin-actin interactions, although it could play a role in forming tetramers. The relative activities of adducin imply that an important role of adducin in cells is to form a complex with the fast growing ends of actin filaments that recruits spectrin and prevents addition or loss of actin subunits.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
D. A. Dansereau and P. Lasko
RanBPM regulates cell shape, arrangement, and capacity of the female germline stem cell niche in Drosophila melanogaster
J. Cell Biol., September 8, 2008; 182(5): 963 - 977.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. D. S. Dixon, D. K. Arneman, A. S. Rachlin, N. R. Sundaresan, M. J. Costello, S. L. Campbell, and C. A. Otey
Palladin Is an Actin Cross-linking Protein That Uses Immunoglobulin-like Domains to Bind Filamentous Actin
J. Biol. Chem., March 7, 2008; 283(10): 6222 - 6231.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. M. Abdi and V. Bennett
Adducin Promotes Micrometer-Scale Organization of {beta}2-Spectrin in Lateral Membranes of Bronchial Epithelial Cells
Mol. Biol. Cell, February 1, 2008; 19(2): 536 - 545.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C.-L. Chen, Y.-T. Hsieh, and H.-C. Chen
Phosphorylation of adducin by protein kinase C{delta} promotes cell motility
J. Cell Sci., April 1, 2007; 120(7): 1157 - 1167.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. A. Kalfa, S. Pushkaran, N. Mohandas, J. H. Hartwig, V. M. Fowler, J. F. Johnson, C. H. Joiner, D. A. Williams, and Y. Zheng
Rac GTPases regulate the morphology and deformability of the erythrocyte cytoskeleton
Blood, December 1, 2006; 108(12): 3637 - 3645.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Manno, Y. Takakuwa, and N. Mohandas
Modulation of Erythrocyte Membrane Mechanical Function by Protein 4.1 Phosphorylation
J. Biol. Chem., March 4, 2005; 280(9): 7581 - 7587.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. L. Barkalow, J. E. Italiano Jr., D. E. Chou, Y. Matsuoka, V. Bennett, and J. H. Hartwig
{alpha}-Adducin dissociates from F-actin and spectrin during platelet activation
J. Cell Biol., May 12, 2003; 161(3): 557 - 570.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
H. Yang, S. C. Francis, K. Sellers, M. DeBarros, C. Sun, C. Sumners, C. M. Ferrario, M. J. Katovich, A. F. Muro, and M. K. Raizada
Hypertension-Linked Decrease in the Expression of Brain {gamma}-Adducin
Circ. Res., October 4, 2002; 91(7): 633 - 639.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. J. Tisdale
Glyceraldehyde-3-phosphate Dehydrogenase Is Phosphorylated by Protein Kinase Ciota /lambda and Plays a Role in Microtubule Dynamics in the Early Secretory Pathway
J. Biol. Chem., January 25, 2002; 277(5): 3334 - 3341.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
V. Bennett and A. J. Baines
Spectrin and Ankyrin-Based Pathways: Metazoan Inventions for Integrating Cells Into Tissues
Physiol Rev, July 1, 2001; 81(3): 1353 - 1392.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. F. Muro, M. L. Marro, S. Gajovic, F. Porro, L. Luzzatto, and F. E. Baralle
Mild spherocytic hereditary elliptocytosis and altered levels of alpha - and gamma -adducins in beta -adducin-deficient mice
Blood, June 15, 2000; 95(12): 3978 - 3985.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Croce, R. Flaumenhaft, M. Rivers, B. Furie, B. C. Furie, I. M. Herman, and D. A. Potter
Inhibition of Calpain Blocks Platelet Secretion, Aggregation, and Spreading
J. Biol. Chem., December 17, 1999; 274(51): 36321 - 36327.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. C. Kiley, K. J. Clark, M. Goodnough, D. R. Welch, and S. Jaken
Protein Kinase C {{delta}} Involvement in Mammary Tumor Cell Metastasis
Cancer Res., July 1, 1999; 59(13): 3230 - 3238.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. Fukata, N. Oshiro, N. Kinoshita, Y. Kawano, Y. Matsuoka, V. Bennett, Y. Matsuura, and K. Kaibuchi
Phosphorylation of Adducin by Rho-Kinase Plays a Crucial Role in Cell Motility
J. Cell Biol., April 19, 1999; 145(2): 347 - 361.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Amann, B. A. Renley, and J. M. Ervasti
A Cluster of Basic Repeats in the Dystrophin Rod Domain Binds F-actin through an Electrostatic Interaction
J. Biol. Chem., October 23, 1998; 273(43): 28419 - 28423.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. van de Water, I. B. Tijdens, A. Verbrugge, M. Huigsloot, A. A. Dihal, J. L. Stevens, S. Jaken, and G. J. Mulder
Cleavage of the Actin-capping Protein alpha -Adducin at Asp-Asp-Ser-Asp633-Ala by Caspase-3 Is Preceded by Its Phosphorylation on Serine 726 in Cisplatin-induced Apoptosis of Renal Epithelial Cells
J. Biol. Chem., August 11, 2000; 275(33): 25805 - 25813.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Hoffmeister, H. Falet, A. Toker, K. L. Barkalow, T. P. Stossel, and J. H. Hartwig
Mechanisms of Cold-induced Platelet Actin Assembly
J. Biol. Chem., June 29, 2001; 276(27): 24751 - 24759.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Spizz and P. J. Blackshear
Overexpression of the Myristoylated Alanine-rich C-kinase Substrate Inhibits Cell Adhesion to Extracellular Matrix Components
J. Biol. Chem., August 17, 2001; 276(34): 32264 - 32273.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.