HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mitoma, J. Articles by Hirabayashi, Y. Search for Related Content PubMed PubMed Citation Articles by Mitoma, J. Articles by Hirabayashi, Y. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 31, 19363-19366, July 31, 1998

COMMUNICATION
Occurrence of an Unusual Phospholipid, Phosphatidyl-L-threonine, in Cultured Hippocampal Neurons
EXOGENOUS L-SERINE IS REQUIRED FOR THE SYNTHESIS OF NEURONAL PHOSPHATIDYL-L-SERINE AND SPHINGOLIPIDS

Junya Mitoma^§, Takeshi Kasama^¶, Shigeki Furuya, and Yoshio Hirabayashi

From the  Laboratory for Cellular Glycobiology, Frontier Research Program and the ^§ Special Postdoctoral Researchers Program, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198, Japan and the ^¶ Instrumental Analysis Research Center for Life Science, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan

We have recently reported that L-serine released from astroglial cells supports the survival and neuritogenesis of hippocampal neurons under a serum- and glia-free culture condition (Mitoma, J., Furuya, S., and Hirabayashi, Y. (1998) Neurosci. Res. 30, 195-199). In this study, we show that exogenous L-serine is required for the synthesis of phosphatidyl-L-serine (PS) and sphingolipids in hippocampal neurons. When hippocampal neurons were maintained under an astroglial cell-free condition, the levels of sphingolipids and phosphatidyl-L-serine in the neurons were greatly reduced in the absence of external L-serine or glycine. Instead, a novel phospholipid appeared just ahead of PS on TLC. This novel lipid was determined to be phosphatidyl-L-threonine by TLC blotting/negative secondary ion mass spectrometry and amino acid analysis. Biochemical studies on rat brain microsomes have indicated that phosphatidyl-L-threonine is synthesized by the base exchange enzyme that is involved in PS synthesis with much lower affinity, that is, approximately of L-serine. Addition of L-serine or glycine to the culture medium restored the synthesis of PS and sphingolipids in the neurons. These observations show that hippocampal neurons require exogenous L-serine for the synthesis of PS and sphingolipids in the absence of astroglial cells and suggested that astroglial cells contribute to neuronal lipid synthesis through the supply of L-serine.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				K. Yoshida, S. Furuya, S. Osuka, J. Mitoma, Y. Shinoda, M. Watanabe, N. Azuma, H. Tanaka, T. Hashikawa, S. Itohara, et al. Targeted Disruption of the Mouse 3-Phosphoglycerate Dehydrogenase Gene Causes Severe Neurodevelopmental Defects and Results in Embryonic Lethality J. Biol. Chem., January 30, 2004; 279(5): 3573 - 3577. [Abstract] [Full Text] [PDF]

				S. Pind, E. Slominski, J. Mauthe, K. Pearlman, K. J. Swoboda, J. A. Wilkins, P. Sauder, and M. R. Natowicz V490M, a Common Mutation in 3-Phosphoglycerate Dehydrogenase Deficiency, Causes Enzyme Deficiency by Decreasing the Yield of Mature Enzyme J. Biol. Chem., February 22, 2002; 277(9): 7136 - 7143. [Abstract] [Full Text] [PDF]

				M. Yamasaki, K. Yamada, S. Furuya, J. Mitoma, Y. Hirabayashi, and M. Watanabe 3-Phosphoglycerate Dehydrogenase, a Key Enzyme for L-Serine Biosynthesis, Is Preferentially Expressed in the Radial Glia/Astrocyte Lineage and Olfactory Ensheathing Glia in the Mouse Brain J. Neurosci., October 1, 2001; 21(19): 7691 - 7704. [Abstract] [Full Text] [PDF]

				S. Furuya, T. Tabata, J. Mitoma, K. Yamada, M. Yamasaki, A. Makino, T. Yamamoto, M. Watanabe, M. Kano, and Y. Hirabayashi L-Serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons PNAS, September 29, 2000; (2000) 200364497. [Abstract] [Full Text]

				H.-Y. Kim, M. Akbar, A. Lau, and L. Edsall Inhibition of Neuronal Apoptosis by Docosahexaenoic Acid (22:6n-3). ROLE OF PHOSPHATIDYLSERINE IN ANTIAPOPTOTIC EFFECT J. Biol. Chem., November 3, 2000; 275(45): 35215 - 35223. [Abstract] [Full Text] [PDF]

				S. Furuya, T. Tabata, J. Mitoma, K. Yamada, M. Yamasaki, A. Makino, T. Yamamoto, M. Watanabe, M. Kano, and Y. Hirabayashi L-Serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons PNAS, October 10, 2000; 97(21): 11528 - 11533. [Abstract] [Full Text] [PDF]