JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakai, T.
Right arrow Articles by Mosher, D. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakai, T.
Right arrow Articles by Mosher, D. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 31, 19378-19382, July 31, 1998

Restoration of beta 1A Integrins is Required for Lysophosphatidic Acid-induced Migration of beta 1-null Mouse Fibroblastic Cells

Takao SakaiDagger , Olivier PeyruchaudDagger , Reinhard Fässler§, and Deane F. MosherDagger

From the Dagger  Departments of Medicine and Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706 and the § Department of Experimental Pathology, Lund University, 221 85 Lund, Sweden

Cells lacking the beta 1 integrin subunit or expressing beta 1A with certain cytoplasmic mutations have poor directed cell migration to platelet-derived growth factor or epidermal growth factor, ligands of receptor tyrosine kinases (Sakai, T., Zhang, Q., Fässler, R., and Mosher, D. F. (1998) J. Cell Biol. 141, 527-538). We investigated the effect of expression of beta 1A integrins on lysophosphatidic acid (LPA)-induced migration of fibroblastic cells derived from beta 1-null mouse embryonic stem cells. These cells expressed edg-2, a G-protein-linked receptor for LPA, as well as the related edg-1 receptor. Cells expressing wild type beta 1A demonstrated enhanced cell migration across filters coated with gelatin or adhesive proteins in response to LPA, whereas beta 1-deficient cells lacked LPA-induced cell migratory ability. Checkerboard analyses indicated that LPA causes both chemotaxis and chemokinesis of beta 1-replete cells. Cells expressing beta 1A with mutations of prolines or tyrosines in conserved cytoplasmic NPXY motifs, threonine in the inter-motif sequence, or a critical aspartic acid in the extracellular domain had low migratory responses to LPA. These findings indicate that active beta 1A integrin is required for cell migration induced by LPA and that the cytoplasmic domain of ligated beta 1A interacts with pathways that are common to both receptor tyrosine kinase and G-protein-linked receptor signaling.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. Pilquil, J. Dewald, A. Cherney, I. Gorshkova, G. Tigyi, D. English, V. Natarajan, and D. N. Brindley
Lipid Phosphate Phosphatase-1 Regulates Lysophosphatidate-induced Fibroblast Migration by Controlling Phospholipase D2-dependent Phosphatidate Generation
J. Biol. Chem., December 15, 2006; 281(50): 38418 - 38429.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Czuchra, H. Meyer, K. R. Legate, C. Brakebusch, and R. Fassler
Genetic analysis of {beta}1 integrin "activation motifs" in mice
J. Cell Biol., September 11, 2006; 174(6): 889 - 899.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. L. Feire, H. Koss, and T. Compton
Cellular integrins function as entry receptors for human cytomegalovirus via a highly conserved disintegrin-like domain
PNAS, October 26, 2004; 101(43): 15470 - 15475.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Han, D. M. Rose, D. G. Woodside, L. E. Goldfinger, and M. H. Ginsberg
Integrin {alpha}4{beta}1-dependent T Cell Migration Requires Both Phosphorylation and Dephosphorylation of the {alpha}4 Cytoplasmic Domain to Regulate the Reversible Binding of Paxillin
J. Biol. Chem., September 12, 2003; 278(37): 34845 - 34853.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. H.J. Danen, P. Sonneveld, C. Brakebusch, R. Fassler, and A. Sonnenberg
The fibronectin-binding integrins {alpha}5{beta}1 and {alpha}v{beta}3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis
J. Cell Biol., December 23, 2002; 159(6): 1071 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. A. Fishman, Y. Liu, S. M. Ellerbroek, and M. S. Stack
Lysophosphatidic Acid Promotes Matrix Metalloproteinase (MMP) Activation and MMP-dependent Invasion in Ovarian Cancer Cells
Cancer Res., April 1, 2001; 61(7): 3194 - 3199.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Sakai, R. Jove, R. Fässler, and D. F. Mosher
Role of the cytoplasmic tyrosines of beta 1A integrins in transformation by v-src
PNAS, March 16, 2001; (2001) 240456398.
[Abstract] [Full Text]

Home page
 Reproductive SciencesHome page
Y. Xu, Y.-j. Xiao, L. M. Baudhuin, and B. M. Schwartz
The Role and Clinical Applications of Bioactive Lysolipids in Ovarian Cancer
Reproductive Sciences, January 1, 2001; 8(1): 1 - 13.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
M. Cooke, T Sakai, and D. Mosher
Contraction of collagen matrices mediated by alpha2beta1A and alpha(v)beta3 integrins
J. Cell Sci., January 7, 2000; 113(13): 2375 - 2383.
[Abstract] [PDF]

Home page
 JCBHome page
C. Gimond, A. van der Flier, S. van Delft, C. Brakebusch, I. Kuikman, J. G. Collard, R. Fassler, and A. Sonnenberg
Induction of Cell Scattering by Expression of {beta}1 Integrins in {beta}1-deficient Epithelial Cells Requires Activation of Members of the Rho Family of GTPases and Downregulation of Cadherin and Catenin Function
J. Cell Biol., December 13, 1999; 147(6): 1325 - 1340.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Wang, J. R. Van Brocklyn, J. P. Hobson, S. Movafagh, Z. Zukowska-Grojec, S. Milstien, and S. Spiegel
Sphingosine 1-Phosphate Stimulates Cell Migration through a Gi-coupled Cell Surface Receptor. POTENTIAL INVOLVEMENT IN ANGIOGENESIS
J. Biol. Chem., December 10, 1999; 274(50): 35343 - 35350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Sakai, J. M. de la Pena, and D. F. Mosher
Synergism among Lysophosphatidic Acid, beta 1A Integrins, and Epidermal Growth Factor or Platelet-derived Growth Factor in Mediation of Cell Migration
J. Biol. Chem., May 28, 1999; 274(22): 15480 - 15486.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Q. Zhang, O. Peyruchaud, K. J. French, M. K. Magnusson, and D. F. Mosher
Sphingosine 1-Phosphate Stimulates Fibronectin Matrix Assembly Through a Rho-Dependent Signal Pathway
Blood, May 1, 1999; 93(9): 2984 - 2990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W. Gruning, T. Arnould, F. Jochimsen, L. Sellin, S. Ananth, E. Kim, and G. Walz
Modulation of renal tubular cell function by RGS3
Am J Physiol Renal Physiol, April 1, 1999; 276(4): F535 - F543.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Sakai, R. Jove, R. Fassler, and D. F. Mosher
Role of the cytoplasmic tyrosines of beta 1A integrins in transformation by v-src
PNAS, March 27, 2001; 98(7): 3808 - 3813.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.