HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Andersson, L. Articles by Freeman, M. W. Search for Related Content PubMed PubMed Citation Articles by Andersson, L. Articles by Freeman, M. W. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 31, 19592-19601, July 31, 1998

Functional Changes in Scavenger Receptor Binding Conformation Are Induced by Charge Mutants Spanning the Entire Collagen Domain

From the Lipid Metabolism Unit and Nessel Gene Therapy Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114

Macrophage scavenger receptors are trimeric integral membrane proteins that bind a diverse array of negatively charged ligands. They have been shown to play a role in the pathogenesis of atherosclerosis and in host responses to microbial infections. Earlier mutational studies demonstrated that the distal segment of the collagen domain of the receptor was critically important for high affinity ligand binding activity. In this study, mutations spanning the entire collagen domain were generated and binding was assayed in transfected cells, as well as in assays employing a secreted, receptor fusion protein. Many of the distal, positively charged C-terminal residues in the type II collagen domain of the receptor, previously reported to be essential for binding at 37 °C, were found not to be critical for binding at 4 °C. Conversely, more proximally charged residues of the collagen receptor that have not been previously mutated were shown to have substantial effects on binding that were also temperature-dependent. These data suggest that scavenger receptor ligand recognition depends on more complex conformational interactions, involving charged residues throughout the entire collagen domain, than was previously recognized.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. R. M. Ojala, T. Pikkarainen, A. Tuuttila, T. Sandalova, and K. Tryggvason Crystal Structure of the Cysteine-rich Domain of Scavenger Receptor MARCO Reveals the Presence of a Basic and an Acidic Cluster That Both Contribute to Ligand Recognition J. Biol. Chem., June 1, 2007; 282(22): 16654 - 16666. [Abstract] [Full Text] [PDF]

				Y. Jiang, P. Oliver, K. E. Davies, and N. Platt Identification and Characterization of Murine SCARA5, a Novel Class A Scavenger Receptor That Is Expressed by Populations of Epithelial Cells J. Biol. Chem., April 28, 2006; 281(17): 11834 - 11845. [Abstract] [Full Text] [PDF]

				G. Tian, D. Wilcockson, V. H. Perry, P. M. Rudd, R. A. Dwek, F. M. Platt, and N. Platt Inhibition of {alpha}-Glucosidases I and II Increases the Cell Surface Expression of Functional Class A Macrophage Scavenger Receptor (SR-A) by Extending Its Half-life J. Biol. Chem., September 17, 2004; 279(38): 39303 - 39309. [Abstract] [Full Text] [PDF]

				M. R. Daws, P. M. Sullam, E. C. Niemi, T. T. Chen, N. K. Tchao, and W. E. Seaman Pattern Recognition by TREM-2: Binding of Anionic Ligands J. Immunol., July 15, 2003; 171(2): 594 - 599. [Abstract] [Full Text] [PDF]

				V. V. Kunjathoor, M. Febbraio, E. A. Podrez, K. J. Moore, L. Andersson, S. Koehn, J. S. Rhee, R. Silverstein, H. F. Hoff, and M. W. Freeman Scavenger Receptors Class A-I/II and CD36 Are the Principal Receptors Responsible for the Uptake of Modified Low Density Lipoprotein Leading to Lipid Loading in Macrophages J. Biol. Chem., December 13, 2002; 277(51): 49982 - 49988. [Abstract] [Full Text] [PDF]

				M. L. Fitzgerald, A. L. Morris, J. S. Rhee, L. P. Andersson, A. J. Mendez, and M. W. Freeman Naturally Occurring Mutations in the Largest Extracellular Loops of ABCA1 Can Disrupt Its Direct Interaction with Apolipoprotein A-I J. Biol. Chem., August 30, 2002; 277(36): 33178 - 33187. [Abstract] [Full Text] [PDF]

				Y. Xu, D. R. Keene, J. M. Bujnicki, M. Hook, and S. Lukomski Streptococcal Scl1 and Scl2 Proteins Form Collagen-like Triple Helices J. Biol. Chem., July 19, 2002; 277(30): 27312 - 27318. [Abstract] [Full Text] [PDF]

				P. Lehtolainen, A. Taskinen, J. Laukkanen, K. J. Airenne, S. Heino, M. Lappalainen, K. Ojala, V. Marjomaki, J. F. Martin, M. S. Kulomaa, et al. Cloning and Characterization of Scavidin, a Fusion Protein for the Targeted Delivery of Biotinylated Molecules J. Biol. Chem., March 1, 2002; 277(10): 8545 - 8550. [Abstract] [Full Text] [PDF]

				B. B. Gowen, T. K. Borg, A. Ghaffar, and E. P. Mayer The collagenous domain of class A scavenger receptors is involved in macrophage adhesion to collagens J. Leukoc. Biol., April 1, 2001; 69(4): 575 - 582. [Abstract] [Full Text]

				X Shi, S Niimi, T Ohtani, and S Machida Characterization of residues and sequences of the carbohydrate recognition domain required for cell surface localization and ligand binding of human lectin-like oxidized LDL receptor J. Cell Sci., January 4, 2001; 114(7): 1273 - 1282. [Abstract] [PDF]

				J. Laukkanen, P. Lehtolainen, P. J. Gough, D. R. Greaves, S. Gordon, and S. Yla-Herttuala Adenovirus-Mediated Gene Transfer of a Secreted Form of Human Macrophage Scavenger Receptor Inhibits Modified Low-Density Lipoprotein Degradation and Foam-Cell Formation in Macrophages Circulation, March 14, 2000; 101(10): 1091 - 1096. [Abstract] [Full Text] [PDF]

				L. G. Fong and D. Le The Processing of Ligands by the Class A Scavenger Receptor Is Dependent on Signal Information Located in the Cytoplasmic Domain J. Biol. Chem., December 17, 1999; 274(51): 36808 - 36816. [Abstract] [Full Text] [PDF]

				P. G. Lysko, J. Weinstock, C. L. Webb, M. E. Brawner, and N. A. Elshourbagy Identification of a Small-Molecule, Nonpeptide Macrophage Scavenger Receptor Antagonist J. Pharmacol. Exp. Ther., June 1, 1999; 289(3): 1277 - 1285. [Abstract] [Full Text]

				M. L. Fitzgerald, A. J. Mendez, K. J. Moore, L. P. Andersson, H. A. Panjeton, and M. W. Freeman ATP-binding Cassette Transporter A1 Contains an NH2-terminal Signal Anchor Sequence That Translocates the Protein's First Hydrophilic Domain to the Exoplasmic Space J. Biol. Chem., April 27, 2001; 276(18): 15137 - 15145. [Abstract] [Full Text] [PDF]