HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kawai, H. Articles by Proia, R. L. Search for Related Content PubMed PubMed Citation Articles by Kawai, H. Articles by Proia, R. L. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 31, 19634-19638, July 31, 1998

Embryonic Stem Cells with a Disrupted GD3 Synthase Gene Undergo Neuronal Differentiation in the Absence of b-Series Gangliosides

From the Section on Biochemical Genetics, Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892

The dramatic changes in the expression of GD3 and other b-series gangliosides during neuronal development and morphogenesis have led to a widely held belief that these gangliosides may be necessary for neuronal differentiation. To determine directly if GD3 and b-series gangliosides are required for neuronal differentiation, we have produced embryonic stem (ES) cells with both alleles of the GD3 synthase gene (GD3S) disrupted by successive rounds of gene targeting. The double-targeted ES cells were deficient in GD3 synthase activity and did not synthesize b-series gangliosides. Despite this deficit, the GD3S(/) ES cells could be induced to undergo neuronal differentiation. Neuronally differentiated wild-type and GD3S(/) ES cells formed a complex neurite network around the embryoid bodies. Both types of neuronal cells expressed the axon-specific cytoskeletal proteins, neurofilament-M, and growth-associated protein-43 as well as the dendrite-specific marker, microtubule-associated protein-2. Our results indicate that GD3 synthase and b-series gangliosides are not necessary for the neuronal differentiation of uncommitted precursor cells.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. Satake, H. Y. Chen, and A. Varki Genes Modulated by Expression of GD3 Synthase in Chinese Hamster Ovary Cells. EVIDENCE THAT THE Tis21 GENE IS INVOLVED IN THE INDUCTION OF GD3 9-O-ACETYLATION J. Biol. Chem., February 28, 2003; 278(10): 7942 - 7948. [Abstract] [Full Text] [PDF]

				T. Kolter, R. L. Proia, and K. Sandhoff Combinatorial Ganglioside Biosynthesis J. Biol. Chem., July 12, 2002; 277(29): 25859 - 25862. [Full Text] [PDF]

				A. Copani, D. Melchiorri, A. Caricasole, F. Martini, P. Sale, R. Carnevale, R. Gradini, M. A. Sortino, L. Lenti, R. De Maria, et al. beta -Amyloid-Induced Synthesis of the Ganglioside Gd3 Is a Requisite for Cell Cycle Reactivation and Apoptosis in Neurons J. Neurosci., May 15, 2002; 22(10): 3963 - 3968. [Abstract] [Full Text] [PDF]

				G. Wu, X. Xie, Z.-H. Lu, and R. W. Ledeen Cerebellar neurons lacking complex gangliosides degenerate in the presence of depolarizing levels of potassium PNAS, December 22, 2000; (2000) 11523698. [Abstract] [Full Text]

				T. Yamashita, R. Wada, T. Sasaki, C. Deng, U. Bierfreund, K. Sandhoff, and R. L. Proia A vital role for glycosphingolipid synthesis during development and differentiation PNAS, August 3, 1999; 96(16): 9142 - 9147. [Abstract] [Full Text] [PDF]

				H. Kawai, M. L. Allende, R. Wada, M. Kono, K. Sango, C. Deng, T. Miyakawa, J. N. Crawley, N. Werth, U. Bierfreund, et al. Mice Expressing Only Monosialoganglioside GM3 Exhibit Lethal Audiogenic Seizures J. Biol. Chem., March 2, 2001; 276(10): 6885 - 6888. [Abstract] [Full Text] [PDF]

				G. Wu, X. Xie, Z.-H. Lu, and R. W. Ledeen Cerebellar neurons lacking complex gangliosides degenerate in the presence of depolarizing levels of potassium PNAS, January 2, 2001; 98(1): 307 - 312. [Abstract] [Full Text] [PDF]