| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 31, 19884-19891, July 31, 1998
From the Department of Molecular Physiology and Biophysics,
Vanderbilt University, School of Medicine,
Nashville, Tennessee 37232-0615
In this report we describe studies which utilized
yeast strains bearing gain and loss of function alleles of
ABF1 in order to attempt to directly implicate Abf1p in
modulating transcription of the TBP-encoding gene, SPT15, in
vivo. We found that overexpression of Abf1p in a yeast cell
increased transcription of the TBP-encoding gene and that this
stimulation depended upon the exact sequence of the Abf1p binding site
(ABF1) present in the gene. Further, in a yeast strain expressing a
temperature sensitive form of Abf1p, occupancy of the chromosomal ABF1
site in the TBP-encoding gene was immediately lost following a
temperature shift. Both results suggest that Abf1p drives transcription
of the TBP-encoding gene. Surprisingly though we found that continuous
ABF1 cis-element occupancy by Abf1p was not acutely required for normal
levels of transcription of either the TBP-encoding gene or other
"Abf1p-driven" genes tested. We propose a model to explain these
results and suggest mechanisms by which Abf1p could activate gene
transcription.
Genetic Tests of the Role of Abf1p in Driving Transcription of
the Yeast TATA Box Bindng Protein-encoding Gene,
SPT15
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  U. Schlecht, I. Erb, P. Demougin, N. Robine, V. Borde, E. v. Nimwegen, A. Nicolas, and M. Primig Genome-wide Expression Profiling, In Vivo DNA Binding Analysis, and Probabilistic Motif Prediction Reveal Novel Abf1 Target Genes during Fermentation, Respiration, and Sporulation in Yeast Mol. Biol. Cell, May 1, 2008; 19(5): 2193 - 2207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yarragudi, L. W. Parfrey, and R. H. Morse Genome-wide analysis of transcriptional dependence and probable target sites for Abf1 and Rap1 in Saccharomyces cerevisiae Nucleic Acids Res., January 12, 2007; 35(1): 193 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yarragudi, T. Miyake, R. Li, and R. H. Morse Comparison of ABF1 and RAP1 in Chromatin Opening and Transactivator Potentiation in the Budding Yeast Saccharomyces cerevisiae Mol. Cell. Biol., October 15, 2004; 24(20): 9152 - 9164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Miyake, J. Reese, C. M. Loch, D. T. Auble, and R. Li Genome-wide Analysis of ARS (Autonomously Replicating Sequence) Binding Factor 1 (Abf1p)-mediated Transcriptional Regulation in Saccharomyces cerevisiae J. Biol. Chem., August 13, 2004; 279(33): 34865 - 34872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Forsberg and P. O. Ljungdahl Genetic and Biochemical Analysis of the Yeast Plasma Membrane Ssy1p-Ptr3p-Ssy5p Sensor of Extracellular Amino Acids Mol. Cell. Biol., February 1, 2001; 21(3): 814 - 826. [Abstract] [Full Text]
|
|
|
|
|
|
L. Yu and R. H. Morse Chromatin Opening and Transactivator Potentiation by RAP1 in Saccharomyces cerevisiae Mol. Cell. Biol., August 1, 1999; 19(8): 5279 - 5288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yu, N. Sabet, A. Chambers, and R. H. Morse The N-terminal and C-terminal Domains of RAP1 Are Dispensable for Chromatin Opening and GCN4-mediated HIS4 Activation in Budding Yeast J. Biol. Chem., August 24, 2001; 276(35): 33257 - 33264. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
