| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 31, 19909-19913, July 31, 1998
From the University of Texas Southwestern Medical Center,
Department of Pharmacology, Dallas, Texas 75235-9041
MEK1 and MEK2 contain a proline-rich insert not
present in any other known MEK (MAP (mitogen-activated protein)/ERK
(extracellular signal-regulated kinase) kinase) family members. We
examined the effect of removing the MEK1 polyproline insert on MEK
activity, its binding to Raf, and its ability to activate ERKs in
cells. Deletion of the insert had no effect on either the activity of MEK1 or on its ability to bind to Raf-1. Both wild type and
constitutively active MEK1 coimmunoprecipitated with Raf-1 whether or
not the insert was present. Deletion of the insert did not reduce
activation of MEK1 by EGF or activated Raf in cells. The proline-rich
insert enhanced the ability of an otherwise equally active MEK1 protein to regulate endogenous ERKs in mammalian cells. Overexpression of
either constitutively active MEK1 lacking the insert or ERK2 compensates for the weaker in vivo activity of the MEK1
deletion mutant. Expression of the insert in cells reduced activation
of ERKs by EGF. We conclude that the proline-rich insert is not the site of the MEK-Raf interaction and that the polyproline insert is
required for its efficient activation of downstream ERKs in cells.
The MEK1 Proline-rich Insert Is Required for Efficient
Activation of the Mitogen-activated Protein Kinases ERK1 and ERK2 in
Mammalian Cells
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Legewie, B. Schoeberl, N. Bluthgen, and H. Herzel Competing Docking Interactions can Bring About Bistability in the MAPK Cascade Biophys. J., October 1, 2007; 93(7): 2279 - 2288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bissonauth, S. Roy, M. Gravel, S. Guillemette, and J. Charron Requirement for Map2k1 (Mek1) in extra-embryonic ectoderm during placentogenesis Development, September 1, 2006; 133(17): 3429 - 3440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Tarrega, P. Rios, R. Cejudo-Marin, C. Blanco-Aparicio, L. van den Berk, J. Schepens, W. Hendriks, L. Tabernero, and R. Pulido ERK2 Shows a Restrictive and Locally Selective Mechanism of Recognition by Its Tyrosine Phosphatase Inactivators Not Shared by Its Activator MEK1 J. Biol. Chem., November 11, 2005; 280(45): 37885 - 37894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Krueger, F.-L. Chou, A. Glading, E. Schaefer, and M. H. Ginsberg Phosphorylation of Phosphoprotein Enriched in Astrocytes (PEA-15) Regulates Extracellular Signal-regulated Kinase-dependent Transcription and Cell Proliferation Mol. Biol. Cell, August 1, 2005; 16(8): 3552 - 3561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. H. Tran, X. Wu, and J. A. Frost B-Raf and Raf-1 Are Regulated by Distinct Autoregulatory Mechanisms J. Biol. Chem., April 22, 2005; 280(16): 16244 - 16253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Lunin, C. Munger, J. Wagner, Z. Ye, M. Cygler, and M. Sacher The Structure of the MAPK Scaffold, MP1, Bound to Its Partner, p14: A COMPLEX WITH A CRITICAL ROLE IN ENDOSOMAL MAP KINASE SIGNALING J. Biol. Chem., May 28, 2004; 279(22): 23422 - 23430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chen, M. Yazicioglu, and M. H. Cobb Characterization of OSR1, a Member of the Mammalian Ste20p/Germinal Center Kinase Subfamily J. Biol. Chem., March 19, 2004; 279(12): 11129 - 11136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Eblen, J. K. Slack-Davis, A. Tarcsafalvi, J. T. Parsons, M. J. Weber, and A. D. Catling Mitogen-Activated Protein Kinase Feedback Phosphorylation Regulates MEK1 Complex Formation and Activation during Cellular Adhesion Mol. Cell. Biol., March 15, 2004; 24(6): 2308 - 2317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-F. Belanger, S. Roy, M. Tremblay, B. Brott, A.-M. Steff, W. Mourad, P. Hugo, R. Erikson, and J. Charron Mek2 Is Dispensable for Mouse Growth and Development Mol. Cell. Biol., July 15, 2003; 23(14): 4778 - 4787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Gopalbhai, G. Jansen, G. Beauregard, M. Whiteway, F. Dumas, C. Wu, and S. Meloche Negative Regulation of MAPKK by Phosphorylation of a Conserved Serine Residue Equivalent to Ser212 of MEK1 J. Biol. Chem., February 28, 2003; 278(10): 8118 - 8125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Delaney, J. A. Printen, H. Chen, E. B. Fauman, and D. T. Dudley Identification of a Novel Mitogen-Activated Protein Kinase Kinase Activation Domain Recognized by the Inhibitor PD 184352 Mol. Cell. Biol., November 1, 2002; 22(21): 7593 - 7602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Eblen, J. K. Slack, M. J. Weber, and A. D. Catling Rac-PAK Signaling Stimulates Extracellular Signal-Regulated Kinase (ERK) Activation by Regulating Formation of MEK1-ERK Complexes Mol. Cell. Biol., September 1, 2002; 22(17): 6023 - 6033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sharma, Veeranna, M. Sharma, N. D. Amin, R. K. Sihag, P. Grant, N. Ahn, A. B. Kulkarni, and H. C. Pant Phosphorylation of MEK1 by cdk5/p35 Down-regulates the Mitogen-activated Protein Kinase Pathway J. Biol. Chem., January 4, 2002; 277(1): 528 - 534. [Abstract] [Full Text]
|
|
|
|
|
|
H. Cha, E. K. Lee, and P. Shapiro Identification of a C-terminal Region That Regulates Mitogen-activated Protein Kinase Kinase-1 Cytoplasmic Localization and ERK Activation J. Biol. Chem., December 14, 2001; 276(51): 48494 - 48501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions Endocr. Rev., April 1, 2001; 22(2): 153 - 183. [Abstract] [Full Text]
|
|
|
|
|
|
M. C. MacNicol, A. J. Muslin, and A. M. MacNicol Disruption of the 14-3-3 Binding Site within the B-Raf Kinase Domain Uncouples Catalytic Activity from PC12 Cell Differentiation J. Biol. Chem., February 11, 2000; 275(6): 3803 - 3809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Abe, M. Okuda, Q. Huang, M. Yoshizumi, and B. C. Berk Reactive Oxygen Species Activate p90 Ribosomal S6 Kinase via Fyn and Ras J. Biol. Chem., January 21, 2000; 275(3): 1739 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-e Xu, J. L. Wilsbacher, T. Collisson, and M. H. Cobb The N-terminal ERK-binding Site of MEK1 Is Required for Efficient Feedback Phosphorylation by ERK2 in Vitro and ERK Activation in Vivo J. Biol. Chem., November 26, 1999; 274(48): 34029 - 34035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Stewart, M. Sundaram, Y. Zhang, J. Lee, M. Han, and K.-L. Guan Kinase Suppressor of Ras Forms a Multiprotein Signaling Complex and Modulates MEK Localization Mol. Cell. Biol., August 1, 1999; 19(8): 5523 - 5534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Schaeffer and M. J. Weber Mitogen-Activated Protein Kinases: Specific Messages from Ubiquitous Messengers Mol. Cell. Biol., April 1, 1999; 19(4): 2435 - 2444. [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Schaeffer, A. D. Catling, S. T. Eblen, L. S. Collier, A. Krauss, and M. J. Weber MP1: A MEK Binding Partner That Enhances Enzymatic Activation of the MAP Kinase Cascade Science, September 11, 1998; 281(5383): 1668 - 1671. [Abstract] [Full Text]
|
|
|
|
|
|
L. Pei Activation of Mitogen-activated Protein Kinase Cascade Regulates Pituitary Tumor-transforming Gene Transactivation Function J. Biol. Chem., September 29, 2000; 275(40): 31191 - 31198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-e Xu, S. Stippec, F. L. Robinson, and M. H. Cobb Hydrophobic as Well as Charged Residues in Both MEK1 and ERK2 Are Important for Their Proper Docking J. Biol. Chem., July 6, 2001; 276(28): 26509 - 26515. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
