HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Song, J. Articles by Yokoyama, K. K. Search for Related Content PubMed PubMed Citation Articles by Song, J. Articles by Yokoyama, K. K. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 32, 20603-20614, August 7, 1998

Genomic Organization and Expression of a Human Gene for Myc-associated Zinc Finger Protein (MAZ)

Jun Song^§, Hiroo Murakami^¶, Hatsumi Tsutsui, Xiaoren Tang, Masatoshi Matsumura^¶, Keiichi Itakura, Ichirou Kanazawa^**, Kailai Sun^§, and Kazunari K. Yokoyama

From the  Tsukuba Life Science Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan, the ^§ Department of Medical Genetics, China Medical University, Shenyang 110001, China, the ^¶ Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan, the  Department of Molecular Genetics, Beckman Research Institute of The City of Hope, Duarte, California 91010, and the ^** Department of Neurology, Institute of Brain Research, Faculty of Medicine, University of Tokyo, Tokyo, Japan

We have cloned and characterized the genomic structure of the human gene for Myc-associated zinc finger protein (MAZ), which is located on chromosome 16p11.2. This gene is transcribed as an mRNA of 2.7 kilobases (kb) that encodes a 60-kDa MAZ protein. A 40-kb cosmid clone was isolated that includes the promoter, five exons, four introns, and one 3'-untranslated region. All exon-intron junction sequences conform to the GT/AG rule. The promoter region has features typical of a housekeeping gene: a high G + C content (88.4%); a high frequency of CpG dinucleotides, in particular within the region 0.5 kb upstream of the site of initiation of translation; and the absence of canonical TATA and CAAT boxes. An S1 nuclease protection assay demonstrated the presence of multiple sites for initiation of transcription around a site 174 nucleotides (nt) upstream of the ATG codon and such expression was reflected by the promoter activity of a MAZ promoter/CAT (chloramphenicol acetyltransferase) reporter gene. Cis-acting positive and negative elements controlling basal transcription of the human MAZ gene were found from nucleotides (nt) 383 to 248 and nt 2500 to 948. Moreover, positive and negative autoregulatory elements were also identified in the regions from nt 248 to 189 and from nt 383 to 248 after co-transfection of HeLa cells with plasmids that carried the MAZ promoter/CAT construct and the MAZ-expression vector. Our results indicate that the 5'-end flanking sequences are responsible for the promoter activities of the MAZ gene.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C. L. Himeda, J. A. Ranish, and S. D. Hauschka Quantitative Proteomic Identification of MAZ as a Transcriptional Regulator of Muscle-Specific Genes in Skeletal and Cardiac Myocytes Mol. Cell. Biol., October 15, 2008; 28(20): 6521 - 6535. [Abstract] [Full Text] [PDF]

				S. L. Palumbo, R. M. Memmott, D. J. Uribe, Y. Krotova-Khan, L. H. Hurley, and S. W. Ebbinghaus A novel G-quadruplex-forming GGA repeat region in the c-myb promoter is a critical regulator of promoter activity Nucleic Acids Res., April 1, 2008; 36(6): 1755 - 1769. [Abstract] [Full Text] [PDF]

				P.-W. Lee, S. Wu, and Y.-M. Lee Differential Expression of {micro}-Opioid Receptor Gene in CXBK and B6 Mice by Sp1 Mol. Pharmacol., December 1, 2004; 66(6): 1580 - 1584. [Abstract] [Full Text] [PDF]

				H. O. Duan and P. J. Simpson-Haidaris Functional Analysis of Interleukin 6 Response Elements (IL-6REs) on the Human {gamma}-Fibrinogen Promoter: BINDING OF HEPATIC Stat3 CORRELATES NEGATIVELY WITH TRANSACTIVATION POTENTIAL OF TYPE II IL-6REs J. Biol. Chem., October 17, 2003; 278(42): 41270 - 41281. [Abstract] [Full Text] [PDF]

				B. K. Ray, R. Murphy, P. Ray, and A. Ray SAF-2, a Splice Variant of SAF-1, Acts as a Negative Regulator of Transcription J. Biol. Chem., November 22, 2002; 277(48): 46822 - 46830. [Abstract] [Full Text] [PDF]

				H.-M. Hu, M. Arcinas, and L. M. Boxer A Myc-associated Zinc Finger Protein-related Factor Binding Site Is Required for the Deregulation of c-myc Expression by the Immunoglobulin Heavy Chain Gene Enhancers in Burkitt's Lymphoma J. Biol. Chem., March 15, 2002; 277(12): 9819 - 9824. [Abstract] [Full Text] [PDF]

				J. Song, H. Ugai, I. Kanazawa, K. Sun, and K. K. Yokoyama Independent Repression of a GC-rich Housekeeping Gene by Sp1 and MAZ Involves the Same cis-Elements J. Biol. Chem., June 1, 2001; 276(23): 19897 - 19904. [Abstract] [Full Text] [PDF]

				J. Song, H. Ugai, K. Ogawa, Y. Wang, A. Sarai, Y. Obata, I. Kanazawa, K. Sun, K. Itakura, and K. K. Yokoyama Two Consecutive Zinc Fingers in Sp1 and in MAZ Are Essential for Interactions with cis-Elements J. Biol. Chem., August 3, 2001; 276(32): 30429 - 30434. [Abstract] [Full Text] [PDF]