|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 32, 20615-20621, August 7, 1998
From the The sodium/myo-inositol cotransporter
is a plasma membrane protein responsible for concentrative cellular
accumulation of myo-inositol in a variety of tissues. When
cells in kidney and brain are exposed to a hyperosmolar salt condition
(hypertonicity) due to the operation of urinary concentration mechanism
and pathological conditions, respectively, they survive the stress of
hypertonicity by raising the cellular concentration of
myo-inositol. Transcription of the
sodium/myo-inositol cotransporter gene is markedly
stimulated in response to hypertonicity, leading to an increase in the
activity of the cotransporter, which in turn drives the osmoprotective accumulation of myo-inositol. To understand the molecular
mechanisms by which hypertonicity stimulates transcription, we analyzed
the 5'-flanking region of the cotransporter gene for
cis-acting regulatory sequences. We identified five
tonicity-responsive enhancers that are scattered over 50 kilobase
pairs. All the enhancers are variations of the same type of enhancer
interacting with the transcription factor named tonicity-responsive
enhancer binding protein. In vivo methylation
experiments demonstrated that exposure of cells to hypertonicity
increases the binding of tonicity-responsive enhancer binding protein
to the enhancer sites, indicating that all of these enhancers are
involved in the transcriptional stimulation. We conclude that the
sodium/myo-inositol cotransporter gene is regulated by a
large region (~50 kilobase pairs) upstream of the gene.
Transcription of the Sodium/myo-Inositol
Cotransporter Gene Is Regulated by Multiple Tonicity-responsive
Enhancers Spread over 50 Kilobase Pairs in the 5'-Flanking Region
,,,, and
Division of Nephrology, Johns Hopkins School
of Medicine, Baltimore, Maryland 21205 and the § Department
of Pediatrics, University of Pennsylvania School of Medicine,
Philadelphia, Pennsylvania, 19104
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Grewal, N. Defamie, X. Zhang, S. De Gois, A. Shawki, B. Mackenzie, C. Chen, H. Varoqui, and J. D. Erickson SNAT2 Amino Acid Transporter Is Regulated by Amino Acids of the SLC6 {gamma}-Aminobutyric Acid Transporter Subfamily in Neocortical Neurons and May Play No Role in Delivering Glutamine for Glutamatergic Transmission J. Biol. Chem., April 24, 2009; 284(17): 11224 - 11236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Hasler Controlled aquaporin-2 expression in the hypertonic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C641 - C653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen Physiology of Cell Volume Regulation in Vertebrates Physiol Rev, January 1, 2009; 89(1): 193 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Rodgaard, K. Schou, M. B. Friis, and E. K. Hoffmann Does the intracellular ionic concentration or the cell water content (cell volume) determine the activity of TonEBP in NIH3T3 cells? Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1528 - C1534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Saito, T. Saito, K. Kasono, H. Tamemoto, M. Kawakami, S. Sasaki, and S.-e Ishikawa Hypotonicity reduces the activity of murine aquaporin-2 promoter induced by dibutyryl cAMP Exp Physiol, October 1, 2008; 93(10): 1147 - 1156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bissonnette, K. Lahjouji, M. J. Coady, and J.-Y. Lapointe Effects of hyperosmolarity on the Na+-myo-inositol cotransporter SMIT2 stably transfected in the Madin-Darby canine kidney cell line Am J Physiol Cell Physiol, September 1, 2008; 295(3): C791 - C799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Xu, C. C. L. Wong, E. H. Y. Tong, S. S. M. Chung, J. R. Yates III, Y. Yin, and B. C. B. Ko Phosphorylation by Casein Kinase 1 Regulates Tonicity-induced Osmotic Response Element-binding Protein/Tonicity Enhancer-binding Protein Nucleocytoplasmic Trafficking J. Biol. Chem., June 20, 2008; 283(25): 17624 - 17634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Lee, M. Kim, Y. S. Im, W. Choi, S. H. Byeon, and H. K. Lee NFAT5 Induction and Its Role in Hyperosmolar Stressed Human Limbal Epithelial Cells Invest. Ophthalmol. Vis. Sci., May 1, 2008; 49(5): 1827 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kosuge, A. I. Chuang, S. Uematsu, K. P. Tan, K. Ohashi, B. C.B. Ko, and S. Ito Discovery of Osmosensitive Transcriptional Regulation of Human Cytochrome P450 3As by the Tonicity-Responsive Enhancer Binding Protein (Nuclear Factor of Activated T Cells 5) Mol. Pharmacol., October 1, 2007; 72(4): 826 - 837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ito, Y. Fujio, K. Takahashi, and J. Azuma Degradation of NFAT5, a Transcriptional Regulator of Osmotic Stress-related Genes, Is a Critical Event for Doxorubicin-induced Cytotoxicity in Cardiac Myocytes J. Biol. Chem., January 12, 2007; 282(2): 1152 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. O'Connor, S. T. Mills, K. A. Jones, S. N. Ho, and G. K. Pavlath A combinatorial role for NFAT5 in both myoblast migration and differentiation during skeletal muscle myogenesis J. Cell Sci., January 1, 2007; 120(1): 149 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Padda, A. Wamsley-Davis, M. C. Gustin, R. Ross, C. Yu, and D. Sheikh-Hamad MEKK3-mediated signaling to p38 kinase and TonE in hypertonically stressed kidney cells Am J Physiol Renal Physiol, October 1, 2006; 291(4): F874 - F881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-T. Tsai, K. G. Danielson, A. Guttapalli, E. Oguz, T. J. Albert, I. M. Shapiro, and M. V. Risbud TonEBP/OREBP Is a Regulator of Nucleus Pulposus Cell Function and Survival in the Intervertebral Disc J. Biol. Chem., September 1, 2006; 281(35): 25416 - 25424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Y. Tong, J.-J. Guo, A.-L. Huang, H. Liu, C.-D. Hu, S. S. M. Chung, and B. C. B. Ko Regulation of Nucleocytoplasmic Trafficking of Transcription Factor OREBP/TonEBP/NFAT5 J. Biol. Chem., August 18, 2006; 281(33): 23870 - 23879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Neuhofer and F.-X. Beck Survival in Hostile Environments: Strategies of Renal Medullary Cells Physiology, June 1, 2006; 21(3): 171 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Hasler, U. S. Jeon, J. A. Kim, D. Mordasini, H. M. Kwon, E. Feraille, and P.-Y. Martin Tonicity-Responsive Enhancer Binding Protein Is an Essential Regulator of Aquaporin-2 Expression in Renal Collecting Duct Principal Cells J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1521 - 1531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yang, A. D. Hodgkinson, P. J. Oates, H. M. Kwon, B. A. Millward, and A. G. Demaine Elevated activity of transcription factor nuclear factor of activated T-cells 5 (NFAT5) and diabetic nephropathy. Diabetes, May 1, 2006; 55(5): 1450 - 1455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, M. Vinciguerra, A. Vandewalle, P.-Y. Martin, and E. Feraille Dual Effects of Hypertonicity on Aquaporin-2 Expression in Cultured Renal Collecting Duct Principal Cells J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1571 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rojek, J. Nielsen, H. L. Brooks, H. Gong, Y.-H. Kim, T.-H. Kwon, J. Frokiaer, and S. Nielsen Altered expression of selected genes in kidney of rats with lithium-induced NDI Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1276 - F1289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Prabhu, R. J. Arner, H. Vunta, and C. C. Reddy Up-regulation of Human myo-Inositol Oxygenase by Hyperosmotic Stress in Renal Proximal Tubular Epithelial Cells J. Biol. Chem., May 20, 2005; 280(20): 19895 - 19901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, B. C. B. Ko, J. Y. Yang, T. T. L. Lam, Z. Jiang, J. Zhang, S. K. Chung, and S. S. M. Chung Transgenic Mice Expressing Dominant-negative Osmotic-response Element-binding Protein (OREBP) in Lens Exhibit Fiber Cell Elongation Defect Associated with Increased DNA Breaks J. Biol. Chem., May 20, 2005; 280(20): 19986 - 19991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kasono, T. Saito, T. Saito, H. Tamemoto, C. Yanagidate, S. Uchida, M. Kawakami, S. Sasaki, and S.-e Ishikawa Hypertonicity regulates the aquaporin-2 promoter independently of arginine vasopressin Nephrol. Dial. Transplant., March 1, 2005; 20(3): 509 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Umenishi, S. Yoshihara, T. Narikiyo, and R. W. Schrier Modulation of Hypertonicity-Induced Aquaporin-1 by Sodium Chloride, Urea, Betaine, and Heat Shock in Murine Renal Medullary Cells J. Am. Soc. Nephrol., March 1, 2005; 16(3): 600 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sheikh-Hamad and M. C. Gustin MAP kinases and the adaptive response to hypertonicity: functional preservation from yeast to mammals Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1102 - F1110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Umenishi and R. W. Schrier Hypertonicity-induced Aquaporin-1 (AQP1) Expression Is Mediated by the Activation of MAPK Pathways and Hypertonicity-responsive Element in the AQP1 Gene J. Biol. Chem., April 25, 2003; 278(18): 15765 - 15770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Storm, E. Klussmann, A. Geelhaar, W. Rosenthal, and K. Maric Osmolality and solute composition are strong regulators of AQP2 expression in renal principal cells Am J Physiol Renal Physiol, January 1, 2003; 284(1): F189 - F198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. B. Ko, A. K. M. Lam, A. Kapus, L. Fan, S. K. Chung, and S. S. M. Chung Fyn and p38 Signaling Are Both Required for Maximal Hypertonic Activation of the Osmotic Response Element-binding Protein/Tonicity-responsive Enhancer-binding Protein (OREBP/TonEBP) J. Biol. Chem., November 22, 2002; 277(48): 46085 - 46092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Trama, W. Y. Go, and S. N. Ho The Osmoprotective Function of the NFAT5 Transcription Factor in T Cell Development and Activation J. Immunol., November 15, 2002; 169(10): 5477 - 5488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Woo, S. D. Lee, K. Y. Na, W. K. Park, and H. M. Kwon TonEBP/NFAT5 Stimulates Transcription of HSP70 in Response to Hypertonicity Mol. Cell. Biol., August 15, 2002; 22(16): 5753 - 5760. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R Alfieri, A. Cavazzoni, P. G. Petronini, M. A Bonelli, A. E Caccamo, A. F Borghetti, and K. P Wheeler Compatible osmolytes modulate the response of porcine endothelial cells to hypertonicity and protect them from apoptosis J. Physiol., April 15, 2002; 540(2): 499 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. CHA, S. K. WOO, K. H. HAN, Y. H. KIM, J. S. HANDLER, J. KIM, and H. M. KWON Hydration Status Affects Nuclear Distribution of Transcription Factor Tonicity Responsive Enhancer Binding Protein in Rat Kidney J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2221 - 2230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Ferraris and A. Garcia-Perez Osmotically Responsive Genes: The Mammalian Osmotic Response Element (ORE) Integr. Comp. Biol., August 1, 2001; 41(4): 734 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsuzaki, T. Suzuki, and K. Takata Hypertonicity-induced expression of aquaporin 3 in MDCK cells Am J Physiol Cell Physiol, July 1, 2001; 281(1): C55 - C63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Franchi-Gazzola, R. Visigalli, V. Dall'Asta, R. Sala, S. K. Woo, H. M. Kwon, G. C. Gazzola, and O. Bussolati Amino acid depletion activates TonEBP and sodium-coupled inositol transport Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1465 - C1474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Graef, J. M. Gastier, U. Francke, and G. R. Crabtree Evolutionary relationships among Rel domains indicate functional diversification by recombination PNAS, May 8, 2001; 98(10): 5740 - 5745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Dahl, J. S. Handler, and H. M. Kwon Hypertonicity-induced phosphorylation and nuclear localization of the transcription factor TonEBP Am J Physiol Cell Physiol, February 1, 2001; 280(2): C248 - C253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Trama, Q. Lu, R. G. Hawley, and S. N. Ho The NFAT-Related Protein NFATL1 (TonEBP/NFAT5) Is Induced Upon T Cell Activation in a Calcineurin-Dependent Manner J. Immunol., November 1, 2000; 165(9): 4884 - 4894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. BAGNASCO, T. PENG, Y. NAKAYAMA, and J. M. SANDS Differential Expression of Individual UT-A Urea Transporter Isoforms in Rat Kidney J. Am. Soc. Nephrol., November 1, 2000; 11(11): 1980 - 1986. [Abstract] [Full Text]
|
|
|
|
|
|
S. K. Woo, S. C. Dahl, J. S. Handler, and H. M. Kwon Bidirectional regulation of tonicity-responsive enhancer binding protein in response to changes in tonicity Am J Physiol Renal Physiol, June 1, 2000; 278(6): F1006 - F1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Hoffert, V. Leitch, P. Agre, and L. S. King Hypertonic Induction of Aquaporin-5 Expression through an ERK-dependent Pathway J. Biol. Chem., March 17, 2000; 275(12): 9070 - 9077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Woo, D. Maouyo, J. S. Handler, and H. M. Kwon Nuclear redistribution of tonicity-responsive enhancer binding protein requires proteasome activity Am J Physiol Cell Physiol, February 1, 2000; 278(2): C323 - C330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tsukaguchi, S. Weremowicz, C. C. Morton, and M. A. Hediger Functional and molecular characterization of the human neutral solute channel aquaporin-9 Am J Physiol Renal Physiol, November 1, 1999; 277(5): F685 - F696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Bai, J. F. Collins, Y. L. Muller, H. Xu, P. R. Kiela, and F. K. Ghishan Characterization of cis-elements required for osmotic response of rat Na+/H+ exchanger-2 (NHE-2) gene Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1999; 277(4): R1112 - R1119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Miyakawa, S. K. Woo, S. C. Dahl, J. S. Handler, and H. M. Kwon Tonicity-responsive enhancer binding protein, a Rel-like protein that stimulates transcription in response to hypertonicity PNAS, March 2, 1999; 96(5): 2538 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nakayama, T. Peng, J. M. Sands, and S. M. Bagnasco The TonE/TonEBP Pathway Mediates Tonicity-responsive Regulation of UT-A Urea Transporter Expression J. Biol. Chem., December 1, 2000; 275(49): 38275 - 38280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Nahm, S. K. Woo, J. S. Handler, and H. M. Kwon Involvement of multiple kinase pathways in stimulation of gene transcription by hypertonicity Am J Physiol Cell Physiol, January 1, 2002; 282(1): C49 - C58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fenton, C. A. Cottingham, G. S. Stewart, A. Howorth, J. A. Hewitt, and C. P. Smith Structure and characterization of the mouse UT-A gene (Slc14a2) Am J Physiol Renal Physiol, April 1, 2002; 282(4): F630 - F638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Alfieri, A. Cavazzoni, P. G. Petronini, M. A. Bonelli, A. E. Caccamo, A. F. Borghetti, and K. P. Wheeler Compatible osmolytes modulate the response of endothelial cells to hypertonicity and protect them from apoptosis J. Physiol., February 22, 2002; (2002) 200101339. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |