| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 33, 20894-20902, August 14, 1998
,,, and
From the The thermodynamics and kinetics of actin
interaction with Arabidopsis thaliana
actin-depolymerizing factor (ADF)1, human ADF, and S6D
mutant ADF1 protein mimicking phosphorylated
(inactive) ADF are examined comparatively. ADFs interact with
ADP·G-actin in rapid equilibrium (k+ = 155 µM Dynamique du Cytosquelette, Laboratoire
d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif-sur-Yvette, France, the § Laboratory of Plant Cell
Biology, Institute of Molecular Agrobiology, National University of
Singapore, Singapore 118240, and the ¶ Laboratory of Plant
Molecular Biology, Rockefeller University, New York,
New York 10021
1·s1 and
k = 16 s1 at 4 °C under
physiological ionic conditions). The kinetics of interaction of plant
and human ADFs with F-actin are slower and exhibit kinetic
cooperativity, consistent with a scheme in which the initial binding of
ADF to two adjacent subunits of the filament nucleates a structural
change that propagates along the filament, allowing faster binding of
ADF in a "zipper" mode. ADF binds in a non-cooperative faster
process to gelsolin-capped filaments or to subtilisin-cleaved F-actin,
which are structurally different from standard filaments (Orlova, A.,
Prochniewicz, E., and Egelman, E. H. (1995) J. Mol.
Biol. 245, 598-607). In contrast, the binding of phalloidin to
F-actin cooperatively inhibits its interaction with ADF. The
ADF-facilitated nucleation of ADP·actin self-assembly indicates that
ADF stabilizes lateral interactions in the filament. Plant and human
ADFs cause only partial depolymerization of F-actin at pH 8, consistent
with identical functions in enhancing F-actin dynamics. Phosphorylation
does not affect ADF activity per se, but decreases its
affinity for actin by 20-fold.
This article has been cited by other articles:
|
|
 |
|
  S. Schaub, J.-J. Meister, and A. B. Verkhovsky Analysis of actin filament network organization in lamellipodia by comparing experimental and simulated images J. Cell Sci., April 15, 2007; 120(8): 1491 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Muhlrad, I. Ringel, D. Pavlov, Y. M. Peyser, and E. Reisler Antagonistic Effects of Cofilin, Beryllium Fluoride Complex, and Phalloidin on Subdomain 2 and Nucleotide-Binding Cleft in F-Actin Biophys. J., December 15, 2006; 91(12): 4490 - 4499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pelikan Conchaudron, D. Didry, K. H. D. Le, E. Larquet, N. Boisset, D. Pantaloni, and M.-F. Carlier Analysis of Tetramethylrhodamine-labeled Actin Polymerization and Interaction with Actin Regulatory Proteins J. Biol. Chem., August 18, 2006; 281(33): 24036 - 24047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Clark, J. Teply, B. K. Haarer, S. C. Viggiano, D. Sept, and D. C. Amberg A Genetic Dissection of Aip1p's Interactions Leads to a Model for Aip1p-Cofilin Cooperative Activities Mol. Biol. Cell, April 1, 2006; 17(4): 1971 - 1984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huang, R. C. Robinson, L. Y. Gao, T. Matsumoto, A. Brunet, L. Blanchoin, and C. J. Staiger Arabidopsis VILLIN1 Generates Actin Filament Cables That Are Resistant to Depolymerization PLANT CELL, February 1, 2005; 17(2): 486 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huang, L. Blanchoin, F. Chaudhry, V. E. Franklin-Tong, and C. J. Staiger A Gelsolin-like Protein from Papaver rhoeas Pollen (PrABP80) Stimulates Calcium-regulated Severing and Depolymerization of Actin Filaments J. Biol. Chem., May 28, 2004; 279(22): 23364 - 23375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ono, K. Mohri, and K. Ono Microscopic Evidence That Actin-interacting Protein 1 Actively Disassembles Actin-depolymerizing Factor/Cofilin-bound Actin Filaments J. Biol. Chem., April 2, 2004; 279(14): 14207 - 14212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Pope, K. M. Zierler-Gould, R. Kuhne, A. G. Weeds, and L. J. Ball Solution Structure of Human Cofilin: ACTIN BINDING, pH SENSITIVITY, AND RELATIONSHIP TO ACTIN-DEPOLYMERIZING FACTOR J. Biol. Chem., February 6, 2004; 279(6): 4840 - 4848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. E. Galkin, A. Orlova, M. S. VanLoock, A. Shvetsov, E. Reisler, and E. H. Egelman ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments J. Cell Biol., December 8, 2003; 163(5): 1057 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mohri and S. Ono Actin filament disassembling activity of Caenorhabditis elegans actin-interacting protein 1 (UNC-78) is dependent on filament binding by a specific ADF/cofilin isoform J. Cell Sci., October 15, 2003; 116(20): 4107 - 4118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ono, M. Parast, C. Alberico, G. M. Benian, and S. Ono Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent processes in Caenorhabditis elegans J. Cell Sci., May 15, 2003; 116(10): 2073 - 2085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Dos Remedios, D. Chhabra, M. Kekic, I. V. Dedova, M. Tsubakihara, D. A. Berry, and N. J. Nosworthy Actin Binding Proteins: Regulation of Cytoskeletal Microfilaments Physiol Rev, April 1, 2003; 83(2): 433 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wiesner, E. Helfer, D. Didry, G. Ducouret, F. Lafuma, M.-F. Carlier, and D. Pantaloni A biomimetic motility assay provides insight into the mechanism of actin-based motility J. Cell Biol., February 3, 2003; 160(3): 387 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Ojala, V. O. Paavilainen, M. K. Vartiainen, R. Tuma, A. G. Weeds, and P. Lappalainen The Two ADF-H Domains of Twinfilin Play Functionally Distinct Roles in Interactions with Actin Monomers Mol. Biol. Cell, November 1, 2002; 13(11): 3811 - 3821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mogilner and L. Edelstein-Keshet Regulation of Actin Dynamics in Rapidly Moving Cells: A Quantitative Analysis Biophys. J., September 1, 2002; 83(3): 1237 - 1258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nyakern-Meazza, K. Narayan, C. E. Schutt, and U. Lindberg Tropomyosin and Gelsolin Cooperate in Controlling the Microfilament System J. Biol. Chem., August 2, 2002; 277(32): 28774 - 28779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. DesMarais, I. Ichetovkin, J. Condeelis, and S. E. Hitchcock-DeGregori Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge J. Cell Sci., January 12, 2002; 115(23): 4649 - 4660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Le Clainche, D. Didry, M.-F. Carlier, and D. Pantaloni Activation of Arp2/3 Complex by Wiskott-Aldrich Syndrome Protein Is Linked to Enhanced Binding of ATP to Arp2 J. Biol. Chem., December 7, 2001; 276(50): 46689 - 46692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Ashworth, R. M. Sandoval, M. Hosford, J. R. Bamburg, and B. A. Molitoris Ischemic injury induces ADF relocalization to the apical domain of rat proximal tubule cells Am J Physiol Renal Physiol, May 1, 2001; 280(5): F886 - F894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. E. Galkin, A. Orlova, N. Lukoyanova, W. Wriggers, and E. H. Egelman Actin Depolymerizing Factor Stabilizes an Existing State of F-Actin and Can Change the Tilt of F-Actin Subunits J. Cell Biol., April 2, 2001; 153(1): 75 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. S. Southwick Gelsolin and ADF/cofilin enhance the actin dynamics of motile cells PNAS, June 20, 2000; 97(13): 6936 - 6938. [Full Text] [PDF]
|
|
|
|
|
|
M.-F. Carlier, F. Ressad, and D. Pantaloni Control of Actin Dynamics in Cell Motility. ROLE OF ADF/COFILIN J. Biol. Chem., November 26, 1999; 274(48): 33827 - 33830. [Full Text] [PDF]
|
|
|
|
|
|
F. Ressad, D. Didry, C. Egile, D. Pantaloni, and M.-F. Carlier Control of Actin Filament Length and Turnover by Actin Depolymerizing Factor (ADF/Cofilin) in the Presence of Capping Proteins and ARP2/3 Complex J. Biol. Chem., July 23, 1999; 274(30): 20970 - 20976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Blanchoin and T. D. Pollard Mechanism of Interaction of Acanthamoeba Actophorin (ADF/Cofilin) with Actin Filaments J. Biol. Chem., May 28, 1999; 274(22): 15538 - 15546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Didry, M.-F. Carlier, and D. Pantaloni Synergy between Actin Depolymerizing Factor/Cofilin and Profilin in Increasing Actin Filament Turnover J. Biol. Chem., October 2, 1998; 273(40): 25602 - 25611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ono, A. McGough, B. J. Pope, V. T. Tolbert, A. Bui, J. Pohl, G. M. Benian, K. M. Gernert, and A. G. Weeds The C-terminal Tail of UNC-60B (Actin Depolymerizing Factor/Cofilin) Is Critical for Maintaining Its Stable Association with F-actin and Is Implicated in the Second Actin-binding Site J. Biol. Chem., February 16, 2001; 276(8): 5952 - 5958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pfannstiel, M. Cyrklaff, A. Habermann, S. Stoeva, G. Griffiths, R. Shoeman, and H. Faulstich Human Cofilin Forms Oligomers Exhibiting Actin Bundling Activity J. Biol. Chem., December 21, 2001; 276(52): 49476 - 49484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Palmgren, P. J. Ojala, M. A. Wear, J. A. Cooper, and P. Lappalainen Interactions with PIP2, ADP-actin monomers, and capping protein regulate the activity and localization of yeast twinfilin J. Cell Biol., October 15, 2001; 155(2): 251 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Vartiainen, T. Mustonen, P. K. Mattila, P. J. Ojala, I. Thesleff, J. Partanen, and P. Lappalainen The Three Mouse Actin-depolymerizing Factor/Cofilins Evolved to Fulfill Cell-Type-specific Requirements for Actin Dynamics Mol. Biol. Cell, January 1, 2002; 13(1): 183 - 194. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
