| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 33, 21040-21053, August 14, 1998
,§,,, and
From the Testin is a testosterone-responsive Sertoli cell
secretory product. In the present study, we demonstrated that the
amount of testin secreted by Sertoli cells in vitro was
comparable with several other Sertoli cell secretory products. However,
virtually no testin was found in the luminal fluid and cytosols of the
testis and epididymis when the intercellular junctions were not
previously disrupted, suggesting that secreted testin may be reabsorbed
by testicular cells in vivo. Studies using Sertoli cells
with and without a cell surface cross-linker and radioiodination in
conjunction with immunoprecipitation illustrated the presence of two
polypeptides of 28 and 45 kDa, which constitute a binding protein
complex that anchors testin onto the cell surface. The 28- and 45-kDa
peptide appear to be residing on and inside the cell surface,
respectively. Immunogold EM studies illustrated testin was abundantly
localized on the Sertoli cell side of the ectoplasmic specialization (a modified adherens junction) surrounding developing spermatids. In
contrast, very few testin gold particles were found at the site of
inter-Sertoli tight junctions. When the inter-Sertoli tight junctions
were formed or disrupted, no significant change in testin expression
was noted. This is in sharp contrast to the disruption of Sertoli-germ
cell junctions, which is accompanied by a surge in testin expression.
These results demonstrate the usefulness of testin in examining
Sertoli-germ cell interactions.
The Population Council, New York, New York
10021 and § Department of Zoology, University of Hong Kong,
Hong Kong, People's Republic of China
This article has been cited by other articles:
|
|
 |
|
  S. J Meachem, S. M Ruwanpura, J. Ziolkowski, J. M Ague, M. K Skinner, and K. L Loveland Developmentally distinct in vivo effects of FSH on proliferation and apoptosis during testis maturation J. Endocrinol., September 1, 2005; 186(3): 429 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. P.Y. Lee, D. D. Mruk, C.-h. Wong, and C. Y. Cheng Regulation of Sertoli-Germ Cell Adherens Junction Dynamics in the Testis Via the Nitric Oxide Synthase (NOS)/cGMP/Protein Kinase G (PRKG)/{beta}-Catenin (CATNB) Signaling Pathway: An In Vitro and In Vivo Study Biol Reprod, September 1, 2005; 73(3): 458 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kleymenova, C. Swanson, K. Boekelheide, and K. W. Gaido Exposure In Utero to Di(n-Butyl) Phthalate Alters the Vimentin Cytoskeleton of Fetal Rat Sertoli Cells and Disrupts Sertoli Cell-Gonocyte Contact Biol Reprod, September 1, 2005; 73(3): 482 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Y. Siu, C.-h. Wong, W. M. Lee, and C. Y. Cheng Sertoli-Germ Cell Anchoring Junction Dynamics in the Testis Are Regulated by an Interplay of Lipid and Protein Kinases J. Biol. Chem., July 1, 2005; 280(26): 25029 - 25047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-h. Wong, D. D. Mruk, M. K. Y. Siu, and C. Y. Cheng Blood-Testis Barrier Dynamics Are Regulated by {alpha}2-Macroglobulin via the c-Jun N-Terminal Protein Kinase Pathway Endocrinology, April 1, 2005; 146(4): 1893 - 1908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Mruk and C. Y. Cheng Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis Endocr. Rev., October 1, 2004; 25(5): 747 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K.Y. Siu and C. Y. Cheng Extracellular Matrix: Recent Advances on Its Role in Junction Dynamics in the Seminiferous Epithelium During Spermatogenesis Biol Reprod, August 1, 2004; 71(2): 375 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. P.Y. Lee and C. Y. Cheng Ectoplasmic specialization, a testis-specific cell-cell actin-based adherens junction type: is this a potential target for male contraceptive development? Hum. Reprod. Update, July 1, 2004; 10(4): 349 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K.Y. Siu and C. Y. Cheng Interactions of Proteases, Protease Inhibitors, and the {beta}1 Integrin/Laminin {gamma}3 Protein Complex in the Regulation of Ectoplasmic Specialization Dynamics in the Rat Testis Biol Reprod, April 1, 2004; 70(4): 945 - 964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Mruk, M. K.Y. Siu, A. M. Conway, N. P.Y. Lee, A. S.N. Lau, and C. Y. Cheng Role of Tissue Inhibitor of Metalloproteases-1 in Junction Dynamics in the Testis J Androl, July 1, 2003; 24(4): 510 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-y. Lui, W. M. Lee, and C. Y. Cheng Sertoli-Germ Cell Adherens Junction Dynamics in the Testis Are Regulated by RhoB GTPase via the ROCK/LIMK Signaling Pathway Biol Reprod, June 1, 2003; 68(6): 2189 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Y. Siu, D. D. Mruk, W. M. Lee, and C. Y. Cheng Adhering Junction Dynamics in the Testis Are Regulated by an Interplay of {beta}1-Integrin and Focal Adhesion Complex-Associated Proteins Endocrinology, May 1, 2003; 144(5): 2141 - 2163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-y. Lui, W. M. Lee, and C. Y. Cheng Transforming Growth Factor {beta}3 Regulates the Dynamics of Sertoli Cell Tight Junctions Via the p38 Mitogen-Activated Protein Kinase Pathway Biol Reprod, May 1, 2003; 68(5): 1597 - 1612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sutou, K. Miwa, T. Matsuura, Y. Kawasaki, Y. Ohinata, and Y. Mitsui Native Tesmin Is a 60-Kilodalton Protein that Undergoes Dynamic Changes in Its Localization During Spermatogenesis in Mice Biol Reprod, May 1, 2003; 68(5): 1861 - 1869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. N. Lau and D. D. Mruk Rab8B GTPase and Junction Dynamics in the Testis Endocrinology, April 1, 2003; 144(4): 1549 - 1563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Cheng, C. H. Cheung, and W. M. Lee Mouse Testin: Complementary DNA Cloning, Genomic Organization, and Characterization of Its Proximal Promoter Region Biol Reprod, April 1, 2003; 68(4): 1376 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-Y. Lui, D. Mruk, W. M Lee, and C. Y. Cheng Sertoli Cell Tight Junction Dynamics: Their Regulation During Spermatogenesis Biol Reprod, April 1, 2003; 68(4): 1087 - 1097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P.Y. Lee, D. Mruk, W. M. Lee, and C. Y. Cheng Is the Cadherin/Catenin Complex a Functional Unit of Cell-Cell Actin-Based Adherens Junctions in the Rat Testis? Biol Reprod, February 1, 2003; 68(2): 489 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Y. Siu, W. M. Lee, and C. Y. Cheng The Interplay of Collagen IV, Tumor Necrosis Factor-{alpha}, Gelatinase B (Matrix Metalloprotease-9), and Tissue Inhibitor of Metalloproteases-1 in the Basal Lamina Regulates Sertoli Cell-Tight Junction Dynamics in the Rat Testis Endocrinology, January 1, 2003; 144(1): 371 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Y. Cheng and D. D. Mruk Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development Physiol Rev, October 1, 2002; 82(4): 825 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.P. Dickinson CYSTEINE PEPTIDASES OF MAMMALS: THEIR BIOLOGICAL ROLES AND POTENTIAL EFFECTS IN THE ORAL CAVITY AND OTHER TISSUES IN HEALTH AND DISEASE Crit. Rev. Oral. Biol. Med., May 1, 2002; 13(3): 238 - 275. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Zhang, A. Z. Wu, Z.-M. Feng, D. Mruk, C. Y. Cheng, and C.-L. C. Chen Gonadotropins, via cAMP, Negatively Regulate GATA-1 Gene Expression in Testicular Cells Endocrinology, March 1, 2002; 143(3): 829 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maekawa, Y. Toyama, M. Yasuda, T. Yagi, and S. Yuasa Fyn Tyrosine Kinase in Sertoli Cells Is Involved in Mouse Spermatogenesis Biol Reprod, January 1, 2002; 66(1): 211 - 221. [Abstract] [Full Text]
|
|
|
|
|
|
N. P.Y. Chung, D. Mruk, M.-y. Mo, W. M. Lee, and C. Y. Cheng A 22-Amino Acid Synthetic Peptide Corresponding to the Second Extracellular Loop of Rat Occludin Perturbs the Blood-Testis Barrier and Disrupts Spermatogenesis Reversibly In Vivo Biol Reprod, November 1, 2001; 65(5): 1340 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Cheng, B. Silvestrini, J. Grima, M.-y. Mo, L.-j. Zhu, E. Johansson, L. Saso, M.-G. Leone, M. Palmery, and D. Mruk Two New Male Contraceptives Exert Their Effects by Depleting Germ Cells Prematurely from the Testis Biol Reprod, August 1, 2001; 65(2): 449 - 461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-Y. Lui, W. M. Lee, and C. Y. Cheng Transforming Growth Factor-{beta}3 Perturbs the Inter-Sertoli Tight Junction Permeability Barrier in Vitro Possibly Mediated via Its Effects on Occludin, Zonula Occludens-1, and Claudin-11 Endocrinology, May 1, 2001; 142(5): 1865 - 1877. [Abstract] [Full Text]
|
|
|
|
|
|
N. P. Y. Chung and C. Y. Cheng Is Cadmium Chloride-Induced Inter-Sertoli Tight Junction Permeability Barrier Disruption a Suitable in Vitro Model to Study the Events of Junction Disassembly during Spermatogenesis in the Rat Testis? Endocrinology, May 1, 2001; 142(5): 1878 - 1888. [Abstract] [Full Text]
|
|
|
|
|
|
J. Grima, B. Silvestrini, and C. Yan Cheng Reversible Inhibition of Spermatogenesis in Rats Using a New Male Contraceptive, 1-(2,4-Dichlorobenzyl)-Indazole-3-Carbohydrazide Biol Reprod, May 1, 2001; 64(5): 1500 - 1508. [Abstract] [Full Text]
|
|
|
|
|
|
J. Grima and C. Y. Cheng Testin Induction: The Role of Cyclic 3',5'-Adenosine Monophosphate/Protein Kinase A Signaling in the Regulation of Basal and Lonidamine-Induced Testin Expression by Rat Sertoli Cells Biol Reprod, December 1, 2000; 63(6): 1648 - 1660. [Abstract] [Full Text]
|
|
|
|
|
|
E. T. Samy, J. C. H. Li, J. Grima, W. M. Lee, B. Silvestrini, and C. Y. Cheng Sertoli Cell Prostaglandin D2 Synthetase Is a Multifunctional Molecule: Its Expression and Regulation Endocrinology, February 1, 2000; 141(2): 710 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Mruk and C. Y. Cheng Sertolin Is a Novel Gene Marker of Cell-Cell Interactions in the Rat Testis J. Biol. Chem., September 17, 1999; 274(38): 27056 - 27068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Syed, E. Gomez, and N. B. Hecht mRNAs Encoding a von Ebner's-like Protein and the Huntington Disease Protein Are Induced in Rat Male Germ Cells by Sertoli Cells J. Biol. Chem., April 16, 1999; 274(16): 10737 - 10742. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
