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J Biol Chem, Vol. 273, Issue 33, 21067-21076, August 14, 1998

Lysine-based Structure Responsible for Selective Mannose Phosphorylation of Cathepsin D and Cathepsin L Defines a Common Structural Motif for Lysosomal Enzyme Targeting

John W. Cuozzo, Kai Tao, Mirek Cygler, John S. Mort^§, and G. Gary Sahagian

From the Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111, the  Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada, and the ^§ Joint Diseases Laboratory, Shriners Hospital for Children, Montreal, Quebec H3G 1A6, Canada

Previous studies have shown that lysine residues on the surface of cathepsins and other lysosomal proteins are a shared component of the recognition structure involved in mannose phosphorylation. In this study, the involvement of specific lysine residues in mannose phosphorylation of cathepsin D was explored by site-directed mutagenesis. Mutation of two lysine residues in the mature portion of the protein, Lys-203 and Lys-293, cooperated to inhibit mannose phosphorylation by 70%. Other positively charged residues could not substitute for lysine at these positions, and comparison of thermal denaturation curves for the wild type and mutant proteins indicated that the inhibition could not be explained by alterations in protein folding. Structural comparisons of the two lysine residues with those required for phosphorylation of cathepsin L, using models generated from recently acquired crystal structures, revealed several relevant similarities. On both molecules, the lysine residues were positioned approximately 34 Å apart (34.06 Å for cathepsin D and 33.80 Å for cathepsin L). When the lysine pairs were superimposed, N-linked glycosylation sites on the two proteins were found to be oriented so that oligosaccharides extending out from the sites could share a common region of space. Further similarities in the local environments of the critical lysines were also observed. These results provide details for a common lysosomal targeting structure based on a specific arrangement of lysine residues with respect to each other and to glycosylation sites on the surface of lysosomal proteins.

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