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J Biol Chem, Vol. 273, Issue 33, 21161-21168, August 14, 1998
From the Department of Oncology, Cross Cancer Institute and
University of Alberta, 11560 University Ave., Edmonton,
Alberta T6G1Z2, Canada
The DEAD box gene, DDX1, is a
putative RNA helicase that is co-amplified with MYCN in a
subset of retinoblastoma (RB) and neuroblastoma (NB) tumors and cell
lines. Although gene amplification usually involves hundreds to
thousands of kilobase pairs of DNA, a number of studies suggest that
co-amplified genes are only overexpressed if they provide a selective
advantage to the cells in which they are amplified. Here, we further
characterize DDX1 by identifying its putative transcription
and translation initiation sites. We analyze DDX1 protein levels in
MYCN/DDX1-amplified NB and RB cell lines using polyclonal
antibodies specific to DDX1 and show that there is a good correlation
with DDX1 gene copy number, DDX1 transcript levels, and DDX1 protein levels in all cell lines studied. DDX1 protein
is found in both the nucleus and cytoplasm of
DDX1-amplified lines but is localized primarily to the
nucleus of nonamplified cells. Our results indicate that DDX1 may be
involved in either the formation or progression of a subset of NB and
RB tumors and suggest that DDX1 normally plays a role in the metabolism
of RNAs located in the nucleus of the cell.
Overexpression of a DEAD Box Protein (DDX1) in Neuroblastoma and
Retinoblastoma Cell Lines
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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