|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 33, 21225-21231, August 14, 1998
From the Departments of The oral anaerobic bacterium Porphyromonas
gingivalis, a major pathogen of advanced adult periodontitis,
produces a novel class of cysteine proteinases in both cell-associated
and secretory forms. A lysine-specific cysteine proteinase
(Lys-gingipain, KGP), as well as an arginine-specific cysteine
proteinase (Arg-gingipain), is a major trypsin-like proteinase of the
organism. Recent studies indicate that the secreted KGP is implicated
in the destruction of periodontal tissue and the disruption of host
defense mechanisms. In this study, we have constructed a KGP-deficient
mutant to determine whether the cell-associated KGP is important for
pathophysiology of the organism. Although the mutant retained the
strong ability to disrupt the bactericidal activity of
polymorphonuclear leukocytes, its hemagglutination activity was reduced
to about one-half that observed with the wild-type strain. More
important, the mutant did not form black-pigmented colonies on blood
agar plates, indicating the defect of hemoglobin adsorption and heme
accumulation. Immunoblot analysis showed that the expression of a
19-kDa hemoglobin receptor protein, which is thought to be responsible
for hemoglobin binding by the organism, was greatly retarded in this
mutant. The mutant also showed a marked decrease in the ability to
degrade fibrinogen. These results suggest the possible involvement of
KGP in the hemoglobin binding and heme accumulation of the organism and
in the bleeding tendency in periodontal pockets.
Involvement of a Lysine-specific Cysteine Proteinase in
Hemoglobin Adsorption and Heme Accumulation by Porphyromonas
gingivalis
,,,
Pharmacology and
¶ Microbiology, Kyushu University Faculty of Dentistry,
Higashi-ku, Fukuoka 812-8582, Japan
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Sato, N. Kido, Y. Murakami, C. I. Hoover, K. Nakayama, and F. Yoshimura Lipopolysaccharide biosynthesis-related genes are required for colony pigmentation of Porphyromonas gingivalis Microbiology, April 1, 2009; 155(4): 1282 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-A. Nguyen, J. Zylicz, P. Szczesny, A. Sroka, N. Hunter, and J. Potempa Verification of a topology model of PorT as an integral outer-membrane protein in Porphyromonas gingivalis Microbiology, February 1, 2009; 155(2): 328 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Naito, H. Hirakawa, A. Yamashita, N. Ohara, M. Shoji, H. Yukitake, K. Nakayama, H. Toh, F. Yoshimura, S. Kuhara, et al. Determination of the Genome Sequence of Porphyromonas gingivalis Strain ATCC 33277 and Genomic Comparison with Strain W83 Revealed Extensive Genome Rearrangements in P. gingivalis DNA Res, August 1, 2008; 15(4): 215 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yamatake, M. Maeda, T. Kadowaki, R. Takii, T. Tsukuba, T. Ueno, E. Kominami, S. Yokota, and K. Yamamoto Role for Gingipains in Porphyromonas gingivalis Traffic to Phagolysosomes and Survival in Human Aortic Endothelial Cells Infect. Immun., May 1, 2007; 75(5): 2090 - 2100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Pathirana, N. M. O'Brien-Simpson, G. C. Brammar, N. Slakeski, and E. C. Reynolds Kgp and RgpB, but Not RgpA, Are Important for Porphyromonas gingivalis Virulence in the Murine Periodontitis Model Infect. Immun., March 1, 2007; 75(3): 1436 - 1442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hashimoto, T. Kadowaki, T. Tsukuba, and K. Yamamoto Selective Proteolysis of Apolipoprotein B-100 by Arg-Gingipain Mediates Atherosclerosis Progression Accelerated by Bacterial Exposure J. Biochem., November 1, 2006; 140(5): 713 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, T. Olczak, H.-C. Guo, D. W. Dixon, and C. A. Genco Identification of Amino Acid Residues Involved in Heme Binding and Hemoprotein Utilization in the Porphyromonas gingivalis Heme Receptor HmuR Infect. Immun., February 1, 2006; 74(2): 1222 - 1232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Soukos, S. Som, A. D. Abernethy, K. Ruggiero, J. Dunham, C. Lee, A. G. Doukas, and J. M. Goodson Phototargeting Oral Black-Pigmented Bacteria Antimicrob. Agents Chemother., April 1, 2005; 49(4): 1391 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sato, E. Sakai, P. D. Veith, M. Shoji, Y. Kikuchi, H. Yukitake, N. Ohara, M. Naito, K. Okamoto, E. C. Reynolds, et al. Identification of a New Membrane-associated Protein That Influences Transport/Maturation of Gingipains and Adhesins of Porphyromonas gingivalis J. Biol. Chem., March 11, 2005; 280(10): 8668 - 8677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Takii, T. Kadowaki, A. Baba, T. Tsukuba, and K. Yamamoto A Functional Virulence Complex Composed of Gingipains, Adhesins, and Lipopolysaccharide Shows High Affinity to Host Cells and Matrix Proteins and Escapes Recognition by Host Immune Systems Infect. Immun., February 1, 2005; 73(2): 883 - 893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kadowaki, A. Baba, N. Abe, R. Takii, M. Hashimoto, T. Tsukuba, S. Okazaki, Y. Suda, T. Asao, and K. Yamamoto Suppression of Pathogenicity of Porphyromonas gingivalis by Newly Developed Gingipain Inhibitors Mol. Pharmacol., December 1, 2004; 66(6): 1599 - 1606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Grenier, S. Roy, F. Chandad, P. Plamondon, M. Yoshioka, K. Nakayama, and D. Mayrand Effect of Inactivation of the Arg- and/or Lys-Gingipain Gene on Selected Virulence and Physiological Properties of Porphyromonas gingivalis Infect. Immun., August 1, 2003; 71(8): 4742 - 4748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Olango, F. Roy, S. M. Sheets, M. K. Young, and H. M. Fletcher Gingipain RgpB Is Excreted as a Proenzyme in the vimA-Defective Mutant Porphyromonas gingivalis FLL92 Infect. Immun., July 1, 2003; 71(7): 3740 - 3747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Paramaesvaran, K.-A. Nguyen, E. Caldon, J. A. McDonald, S. Najdi, G. Gonzaga, D. B. Langley, A. DeCarlo, M. J. Crossley, N. Hunter, et al. Porphyrin-Mediated Cell Surface Heme Capture from Hemoglobin by Porphyromonas gingivalis J. Bacteriol., April 15, 2003; 185(8): 2528 - 2537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. DeCarlo, Y. Huang, C. A. Collyer, D. B. Langley, and J. Katz Feasibility of an HA2 Domain-Based Periodontitis Vaccine Infect. Immun., January 1, 2003; 71(1): 562 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Curtis, J. Aduse Opoku, M. Rangarajan, A. Gallagher, J. A. C. Sterne, C. R. Reid, H. E. A. Evans, and B. Samuelsson Attenuation of the Virulence of Porphyromonas gingivalis by Using a Specific Synthetic Kgp Protease Inhibitor Infect. Immun., December 1, 2002; 70(12): 6968 - 6975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bosch, M. E. Garrido, M. Llagostera, A. M. Perez de Rozas, I. Badiola, and J. Barbe Characterization of the Pasteurella multocida hgbA Gene Encoding a Hemoglobin-Binding Protein Infect. Immun., November 1, 2002; 70(11): 5955 - 5964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Rajapakse, N. M. O'Brien-Simpson, N. Slakeski, B. Hoffmann, and E. C. Reynolds Immunization with the RgpA-Kgp Proteinase-Adhesin Complexes of Porphyromonas gingivalis Protects against Periodontal Bone Loss in the Rat Periodontitis Model Infect. Immun., May 1, 2002; 70(5): 2480 - 2486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shoji, D. B. Ratnayake, Y. Shi, T. Kadowaki, K. Yamamoto, F. Yoshimura, A. Akamine, M. A. Curtis, and K. Nakayama Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: cell surface polysaccharide as an anchorage for gingipains Microbiology, April 1, 2002; 148(4): 1183 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. O'Brien-Simpson, R. A. Paolini, B. Hoffmann, N. Slakeski, S. G. Dashper, and E. C. Reynolds Role of RgpA, RgpB, and Kgp Proteinases in Virulence of Porphyromonas gingivalis W50 in a Murine Lesion Model Infect. Immun., December 1, 2001; 69(12): 7527 - 7534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Gibson III and C. A. Genco Prevention of Porphyromonas gingivalis-Induced Oral Bone Loss following Immunization with Gingipain R1 Infect. Immun., December 1, 2001; 69(12): 7959 - 7963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Olczak, D. W. Dixon, and C. A. Genco Binding Specificity of the Porphyromonas gingivalis Heme and Hemoglobin Receptor HmuR, Gingipain K, and Gingipain R1 for Heme, Porphyrins, and Metalloporphyrins J. Bacteriol., October 1, 2001; 183(19): 5599 - 5608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sroka, M. Sztukowska, J. Potempa, J. Travis, and C. A. Genco Degradation of Host Heme Proteins by Lysine- and Arginine-Specific Cysteine Proteinases (Gingipains) of Porphyromonas gingivalis J. Bacteriol., October 1, 2001; 183(19): 5609 - 5616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nisapakultorn, K. F. Ross, and M. C. Herzberg Calprotectin Expression In Vitro by Oral Epithelial Cells Confers Resistance to Infection by Porphyromonas gingivalis Infect. Immun., July 1, 2001; 69(7): 4242 - 4247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kuboniwa, A. Amano, S. Shizukuishi, I. Nakagawa, and S. Hamada Specific Antibodies to Porphyromonas gingivalis Lys-Gingipain by DNA Vaccination Inhibit Bacterial Binding to Hemoglobin and Protect Mice from Infection Infect. Immun., May 1, 2001; 69(5): 2972 - 2979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Abaibou, Z. Chen, G. J. Olango, Y. Liu, J. Edwards, and H. M. Fletcher vimA Gene Downstream of recA Is Involved in Virulence Modulation in Porphyromonas gingivalis W83 Infect. Immun., January 1, 2001; 69(1): 325 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.A. Curtis, J. Aduse-Opoku, and M. Rangarajan Cysteine Proteases of Porphyromonas Gingivalis Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(3): 192 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Simpson, T. Olczak, and C. A. Genco Characterization and Expression of HmuR, a TonB-Dependent Hemoglobin Receptor of Porphyromonas gingivalis J. Bacteriol., October 15, 2000; 182(20): 5737 - 5748. [Abstract] [Full Text]
|
|
|
|
|
|
J. Aduse-Opoku, N. N. Davies, A. Gallagher, A. Hashim, H. E. A. Evans, M. Rangarajan, J. M. Slaney, and M. A. Curtis Generation of Lys-gingipain protease activity in Porphyromonas gingivalis W50 is independent of Arg-gingipain protease activities Microbiology, August 1, 2000; 146(8): 1933 - 1940. [Abstract] [Full Text]
|
|
|
|
|
|
T. Chen, H. Dong, Y. P. Tang, M. M. Dallas, M. H. Malamy, and M. J. Duncan Identification and Cloning of Genes from Porphyromonas gingivalis after Mutagenesis with a Modified Tn4400 Transposon from Bacteroides fragilis Infect. Immun., January 1, 2000; 68(1): 420 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Abiko Passive Immunization Against Dental Caries and Periodontal Disease: Development of Recombinant and Human Monoclonal Antibodies Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(2): 140 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Simpson, C.-Y. Wang, J. Mikolajczyk-Pawlinska, J. Potempa, J. Travis, V. C. Bond, and C. A. Genco Transposition of the Endogenous Insertion Sequence Element IS1126 Modulates Gingipain Expression in Porphyromonas gingivalis Infect. Immun., October 1, 1999; 67(10): 5012 - 5020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chen and H. K. Kuramitsu Molecular Mechanism for the Spontaneous Generation of Pigmentless Porphyromonas gingivalis Mutants Infect. Immun., September 1, 1999; 67(9): 4926 - 4930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Lewis, J. A. Dawson, J. C. Hannis, D. Muddiman, and F. L. Macrina Hemoglobinase Activity of the Lysine Gingipain Protease (Kgp) of Porphyromonas gingivalis W83 J. Bacteriol., August 15, 1999; 181(16): 4905 - 4913. [Abstract] [Full Text]
|
|
|
|
|
|
M. C. Lynch and H. K. Kuramitsu Role of Superoxide Dismutase Activity in the Physiology of Porphyromonas gingivalis Infect. Immun., July 1, 1999; 67(7): 3367 - 3375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, D. B. Ratnayake, K. Okamoto, N. Abe, K. Yamamoto, and K. Nakayama Genetic Analyses of Proteolysis, Hemoglobin Binding, and Hemagglutination of Porphyromonas gingivalis. CONSTRUCTION OF MUTANTS WITH A COMBINATION OF rgpA, rgpB, kgp, AND hagA J. Biol. Chem., June 18, 1999; 274(25): 17955 - 17960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. DeCarlo, M. Paramaesvaran, P. L. W. Yun, C. Collyer, and N. Hunter Porphyrin-Mediated Binding to Hemoglobin by the HA2 Domain of Cysteine Proteinases (Gingipains) and Hemagglutinins from the Periodontal Pathogen Porphyromonas gingivalis J. Bacteriol., June 15, 1999; 181(12): 3784 - 3791. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Shibata, M. Hayakawa, H. Takiguchi, T. Shiroza, and Y. Abiko Determination and Characterization of the Hemagglutinin-associated Short Motifs Found in Porphyromonas gingivalis Multiple Gene Products J. Biol. Chem., February 19, 1999; 274(8): 5012 - 5020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kadowaki, K. Nakayama, F. Yoshimura, K. Okamoto, N. Abe, and K. Yamamoto Arg-gingipain Acts as a Major Processing Enzyme for Various Cell Surface Proteins in Porphyromonas gingivalis J. Biol. Chem., October 30, 1998; 273(44): 29072 - 29076. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |