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J Biol Chem, Vol. 273, Issue 33, 21324-21331, August 14, 1998
§,
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From the Using antibodies against synaptic protein
preparations, we cloned the cDNA of a new
Ca2+-binding protein. Its C-terminal portion displays
significant similarity with calmodulin and contains two EF-hand motifs.
The corresponding mRNA is highly expressed in rat brain, primarily in cerebral cortex, hippocampus, and cerebellum; its expression appears
to be restricted to neurons. Transcript levels increase during
postnatal development. A recombinant C-terminal protein fragment binds
Ca2+ as indicated by a Ca2+-induced mobility
shift in SDS-polyacrylamide gel electrophoresis. Antisera generated
against the bacterial fusion protein recognize a brain-specific protein
doublet with apparent molecular masses of 33 and 36 kDa. These data are
confirmed by in vitro translation, which generates a single
36-kDa polypeptide, and by the heterologous expression in 293 cells,
which yields a 33/36-kDa doublet comparable to that found in brain. On
two-dimensional gels, the 33-kDa band separates into a chain of spots
plausibly due to differential phosphorylation. This view is supported
by in situ phosphorylation studies in hippocampal slices.
Most of the immunoreactivity is detectable in cytoskeletal preparations
with a further enrichment in the synapse-associated cytomatrix. These
biochemical data, together with the ultra-structural localization in
dendrites and the postsynaptic density, strongly suggest an association
with the somato-dendritic cytoskeleton. Therefore, this novel
Ca2+-binding protein was named caldendrin.
AG Molecular and Cellular Neurobiology,
Institute for Medical Psychology, Otto-von-Guericke-University, 39120 Magdeburg, Germany, the § Department of Neurochemistry and
Molecular Biology, Leibniz Institute for Neurobiology, 39118 Magdeburg,
Germany, the ¶ AG Molecular Neuroendocrinology, Institute for
Anatomy, Westfälische Wilhelms-University, 48149 Münster,
Germany, the Institute for Pharmacology and Toxicology,
Otto-von-Guericke-University, 39120 Magdeburg, Germany, and the
** Department of Neurobiology, University of Alabama at Birmingham,
Birmingham, Alabama 35294-0027
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