| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 33, 21342-21351, August 14, 1998
From the Trauma Research Program and Department of Laboratory
Medicine and Pathobiology, Sunnybrook Health Science Centre, University
of Toronto, Toronto, Ontario M4N 3M5, Canada
Versican is a member of the large aggregating
chondroitin sulfate proteoglycan family. We have expressed in NIH3T3
fibroblasts a recombinant versican mini-gene comprising the G1 and G3
domains and 15% of the CS domain. We observed that expression of the
mini-versican gene stimulated cell proliferation as determined by cell
counting and cell cycle analysis. Addition of exogenous mini-versican
protein to cultured cells produced the same result. The effects of the mini-versican were greatly reduced when the G3 domain was deleted. Expression of the G3 domain alone promotes cell proliferation, and
addition of purified G3 gene products to NIH3T3 fibroblasts and
cultured chicken fibroblasts enhances cell growth. Further, deletion of
the epidermal growth factor (EGF)-like motifs in the versican G3 domain
reduced the effects of the mini-versican on cell proliferation. In the
presence of the purified mini-versican protein, antisense
oligonucleotides to the EGF receptor inhibited proliferation of NIH3T3
fibroblasts, compared with control sense oligonucleotides. Taken
together, these results imply that versican enhances cell
proliferation, and this effect is mediated, at least in part, by the
action of versican EGF-like motifs on endogenous EGF receptor.
The G3 Domain of Versican Enhances Cell Proliferation via
Epidermial Growth Factor-like Motifs
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. T. Read, M. Rahmani, S. Boroomand, S. Allahverdian, B. M. McManus, and P. S. Rennie Androgen Receptor Regulation of the Versican Gene through an Androgen Response Element in the Proximal Promoter J. Biol. Chem., November 2, 2007; 282(44): 31954 - 31963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pukkila, A. Kosunen, K. Ropponen, J. Virtaniemi, J. Kellokoski, E. Kumpulainen, R. Pirinen, J. Nuutinen, R. Johansson, and V.-M. Kosma High stromal versican expression predicts unfavourable outcome in oral squamous cell carcinoma J. Clin. Pathol., March 1, 2007; 60(3): 267 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-Y. Xiang, H. Dong, Y. Wan, J. Li, A. Yee, B. B. Yang, and W.-Y. Lu Versican G3 Domain Regulates Neurite Growth and Synaptic Transmission of Hippocampal Neurons by Activation of Epidermal Growth Factor Receptor J. Biol. Chem., July 14, 2006; 281(28): 19358 - 19368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-S. Zheng, M. Reis, C. Sparling, D. Y. Lee, D. P. La Pierre, C.-K. A. Wong, Z. Deng, S. Kahai, J. Wen, and B. B. Yang Versican G3 Domain Promotes Blood Coagulation through Suppressing the Activity of Tissue Factor Pathway Inhibitor-1 J. Biol. Chem., March 24, 2006; 281(12): 8175 - 8182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yamagata and J. R. Sanes Versican in the Developing Brain: Lamina-Specific Expression in Interneuronal Subsets and Role in Presynaptic Maturation J. Neurosci., September 14, 2005; 25(37): 8457 - 8467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Kozma, K. Olczyk, G. Wisowski, A. Glowacki, and R. Bobinski Alterations in the Extracellular Matrix Proteoglycan Profile in Dupuytren's Contracture Affect the Palmar Fascia J. Biochem., April 1, 2005; 137(4): 463 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Sheng, G. Wang, Y. Wang, J. Liang, J. Wen, P.-S. Zheng, Y. Wu, V. Lee, J. Slingerland, D. Dumont, et al. The Roles of Versican V1 and V2 Isoforms in Cell Proliferation and Apoptosis Mol. Biol. Cell, March 1, 2005; 16(3): 1330 - 1340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Kenagy, J. W. Fischer, S. Lara, J. D. Sandy, A. W. Clowes, and T. N. Wight Accumulation and Loss of Extracellular Matrix During Shear Stress-mediated Intimal Growth and Regression in Baboon Vascular Grafts J. Histochem. Cytochem., January 1, 2005; 53(1): 131 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-S. Zheng, D. Vais, D. LaPierre, Y.-Y. Liang, V. Lee, B. L. Yang, and B. B. Yang PG-M/versican binds to P-selectin glycoprotein ligand-1 and mediates leukocyte aggregation J. Cell Sci., November 15, 2004; 117(24): 5887 - 5895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M J Pukkila, A S T Kosunen, J A Virtaniemi, E J Kumpulainen, R T Johansson, J K Kellokoski, J Nuutinen, and V-M Kosma Versican expression in pharyngeal squamous cell carcinoma: an immunohistochemical study J. Clin. Pathol., July 1, 2004; 57(7): 735 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Wight and M. J. Merrilees Proteoglycans in Atherosclerosis and Restenosis: Key Roles for Versican Circ. Res., May 14, 2004; 94(9): 1158 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, L. Chen, L. Cao, W. Sheng, and B. B. Yang Overexpression of the C-terminal PG-M/versican domain impairs growth of tumor cells by intervening in the interaction between epidermal growth factor receptor and {beta}1-integrin J. Cell Sci., May 1, 2004; 117(11): 2227 - 2237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, W. Sheng, L. Chen, H. Dong, V. Lee, F. Lu, C. S. Wong, W.-Y. Lu, and B. B. Yang Versican V1 Isoform Induces Neuronal Differentiation and Promotes Neurite Outgrowth Mol. Biol. Cell, May 1, 2004; 15(5): 2093 - 2104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Day, A. I. Olin, A. D. Murdoch, A. Canfield, T. Sasaki, R. Timpl, T. E. Hardingham, and A. Aspberg Alternative Splicing in the Aggrecan G3 Domain Influences Binding Interactions with Tenascin-C and Other Extracellular Matrix Proteins J. Biol. Chem., March 26, 2004; 279(13): 12511 - 12518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Spicer, A. Joo, and R. A. Bowling Jr. A Hyaluronan Binding Link Protein Gene Family Whose Members Are Physically Linked Adjacent to Chrondroitin Sulfate Proteoglycan Core Protein Genes: THE MISSING LINKS J. Biol. Chem., May 30, 2003; 278(23): 21083 - 21091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Lee, L. Chen, F. Paiwand, L. Cao, Y. Wu, R. Inman, M. E. Adams, and B. B. Yang Cleavage of the Carboxyl Tail from the G3 Domain of Aggrecan but Not Versican and Identification of the Amino Acids Involved in the Degradation J. Biol. Chem., June 14, 2002; 277(25): 22279 - 22288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, L. Chen, P.-S. Zheng, and B. B. Yang beta 1-Integrin-mediated Glioma Cell Adhesion and Free Radical-induced Apoptosis Are Regulated by Binding to a C-terminal Domain of PG-M/Versican J. Biol. Chem., March 29, 2002; 277(14): 12294 - 12301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Touab, J. Villena, C. Barranco, M. Arumi-Uria, and A. Bassols Versican Is Differentially Expressed in Human Melanoma and May Play a Role in Tumor Development Am. J. Pathol., February 1, 2002; 160(2): 549 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, L. Cao, C. G. Kiani, B. L. Yang, and B. B. Yang The G3 Domain of Versican Inhibits Mesenchymal Chondrogenesis via the Epidermal Growth Factor-like Motifs J. Biol. Chem., December 4, 1998; 273(49): 33054 - 33063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Yang, L. Cao, C. Kiani, V. Lee, Y. Zhang, M. E. Adams, and B. B. Yang Tandem Repeats Are Involved in G1 Domain Inhibition of Versican Expression and Secretion and the G3 Domain Enhances Glycosaminoglycan Modification and Product Secretion via the Complement-binding Protein-like Motif J. Biol. Chem., July 7, 2000; 275(28): 21255 - 21261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Rodriguez-Manzaneque, A. B. Milchanowski, E. K. Dufour, R. Leduc, and M. L. Iruela-Arispe Characterization of METH-1/ADAMTS1 Processing Reveals Two Distinct Active Forms J. Biol. Chem., October 20, 2000; 275(43): 33471 - 33479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kawashima, M. Hirose, J. Hirose, D. Nagakubo, A. H. K. Plaas, and M. Miyasaka Binding of a Large Chondroitin Sulfate/Dermatan Sulfate Proteoglycan, Versican, to L-selectin, P-selectin, and CD44 J. Biol. Chem., November 3, 2000; 275(45): 35448 - 35456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, Y. Zhang, L. Cao, L. Chen, V. Lee, P.-S. Zheng, C. Kiani, M. E. Adams, L. C. Ang, F. Paiwand, et al. Identification of the Motif in Versican G3 Domain That Plays a Dominant-negative Effect on Astrocytoma Cell Proliferation through Inhibiting Versican Secretion and Binding J. Biol. Chem., April 20, 2001; 276(17): 14178 - 14186. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
