HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) An addition or correction has been published Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thiriet, C. Articles by Hayes, J. J. Search for Related Content PubMed PubMed Citation Articles by Thiriet, C. Articles by Hayes, J. J. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 33, 21352-21358, August 14, 1998

Functionally Relevant Histone-DNA Interactions Extend Beyond the Classically Defined Nucleosome Core Region

From the Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642

We demonstrate that core histones can affect the accessibility of a DNA element positioned outside of the classically defined nucleosome core region. The distance between a well positioned nucleosome and the binding site for the 5 S-specific transcription factor TFIIIA was systematically varied and the relative binding affinity for TFIIIA determined. We found that core histone-DNA interactions attenuate the affinity of TFIIIA for its cognate DNA element by a factor of 50-100-fold even when the critical binding region lies well outside of the classically defined nucleosome core region. These results have implications for the validity of parallels drawn between the accessibility of general nucleases to DNA sequences in chromatin and the activity of actual sequence-specific DNA binding factors.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Z. Yang, C. Zheng, and J. J. Hayes The Core Histone Tail Domains Contribute to Sequence-dependent Nucleosome Positioning J. Biol. Chem., March 16, 2007; 282(11): 7930 - 7938. [Abstract] [Full Text] [PDF]

				Z. Yang, C. Zheng, C. Thiriet, and J. J. Hayes The Core Histone N-Terminal Tail Domains Negatively Regulate Binding of Transcription Factor IIIA to a Nucleosome Containing a 5S RNA Gene via a Novel Mechanism Mol. Cell. Biol., January 1, 2005; 25(1): 241 - 249. [Abstract] [Full Text] [PDF]

				J. M. Vitolo, Z. Yang, R. Basavappa, and J. J. Hayes Structural Features of Transcription Factor IIIA Bound to a Nucleosome in Solution Mol. Cell. Biol., January 15, 2004; 24(2): 697 - 707. [Abstract] [Full Text] [PDF]

				S. Aoyagi, P. A. Wade, and J. J. Hayes Nucleosome Sliding Induced by the xMi-2 Complex Does Not Occur Exclusively via a Simple Twist-diffusion Mechanism J. Biol. Chem., August 15, 2003; 278(33): 30562 - 30568. [Abstract] [Full Text] [PDF]

				S. Aoyagi and J. J. Hayes hSWI/SNF-Catalyzed Nucleosome Sliding Does Not Occur Solely via a Twist-Diffusion Mechanism Mol. Cell. Biol., November 1, 2002; 22(21): 7484 - 7490. [Abstract] [Full Text] [PDF]

				D. Angelov, J. M. Vitolo, V. Mutskov, S. Dimitrov, and J. J. Hayes Preferential interaction of the core histone tail domains with linker DNA PNAS, May 24, 2001; (2001) 121171498. [Abstract] [Full Text] [PDF]

				R. Reeves, W. J. Leonard, and M. S. Nissen Binding of HMG-I(Y) Imparts Architectural Specificity to a Positioned Nucleosome on the Promoter of the Human Interleukin-2 Receptor alpha Gene Mol. Cell. Biol., July 1, 2000; 20(13): 4666 - 4679. [Abstract] [Full Text]

				J. M. Vitolo, C. Thiriet, and J. J. Hayes The H3-H4 N-Terminal Tail Domains Are the Primary Mediators of Transcription Factor IIIA Access to 5S DNA within a Nucleosome Mol. Cell. Biol., March 15, 2000; 20(6): 2167 - 2175. [Abstract] [Full Text]

				D. Angelov, J. M. Vitolo, V. Mutskov, S. Dimitrov, and J. J. Hayes Preferential interaction of the core histone tail domains with linker DNA PNAS, June 5, 2001; 98(12): 6599 - 6604. [Abstract] [Full Text] [PDF]