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J Biol Chem, Vol. 273, Issue 34, 21447-21450, August 21, 1998
From the Department of Molecular Medicine/Institute of
Biotechnology, University of Texas Health Science Center at San
Antonio, San Antonio, Texas 78245-3207
Genetic studies in yeast have indicated a role of
the RAD50 and MRE11 genes in homologous
recombination, telomere length maintenance, and DNA repair
processes. Here, we purify from nuclear extract of Raji cells a
complex consisting of human Rad50, Mre11, and another protein factor
with a size of about 95 kDa (p95), which is likely to be Nibrin, the
protein encoded by the gene mutated in Nijmegen breakage syndrome. We
show that the Rad50-Mre11-p95 complex possesses
manganese-dependent single-stranded DNA endonuclease and 3'
to 5' exonuclease activities. These nuclease activities are likely to
be important for recombination, repair, and genomic stability.
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