| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 34, 21759-21768, August 21, 1998
From the Departments of a Physiology and h Biology,
Dartmouth Medical School, Hanover, New Hampshire 03755, the
g Department of Physiology, Johns Hopkins University, School of
Medicine, Baltimore, Maryland 21205, and the d Department of
Physiology and Biophysics, University of Alabama at Birmingham,
Birmingham, Alabama 35294
The mechanism by which cAMP stimulates cystic
fibrosis transmembrane conductance regulator (CFTR)-mediated chloride
(Cl
) secretion is cell type-specific. By using
Madin-Darby canine kidney (MDCK) type I epithelial cells as a model, we
tested the hypothesis that cAMP stimulates Cl secretion
by stimulating CFTR Cl channel trafficking from an
intracellular pool to the apical plasma membrane. To this end, we
generated a green fluorescent protein (GFP)-CFTR expression vector in
which GFP was linked to the N terminus of CFTR. GFP did not alter CFTR
function in whole cell patch-clamp or planar lipid bilayer experiments.
In stably transfected MDCK type I cells, GFP-CFTR localization was
substratum-dependent. In cells grown on glass coverslips,
GFP-CFTR was polarized to the basolateral membrane, whereas in cells
grown on permeable supports, GFP-CFTR was polarized to the apical
membrane. Quantitative confocal fluorescence microscopy and surface
biotinylation experiments demonstrated that cAMP did not stimulate
detectable GFP-CFTR translocation from an intracellular pool to the
apical membrane or regulate GFP-CFTR endocytosis. Disruption of the
microtubular cytoskeleton with colchicine did not affect
cAMP-stimulated Cl secretion or GFP-CFTR expression in
the apical membrane. We conclude that cAMP stimulates CFTR-mediated
Cl secretion in MDCK type I cells by activating channels
resident in the apical plasma membrane.
This article has been cited by other articles:
|
|
 |
|
  B. K. Berdiev, E. Cormet-Boyaka, A. Tousson, Y. J. Qadri, H. M. J. Oosterveld-Hut, J. S. Hong, P. A. Gonzales, C. M. Fuller, E. J. Sorscher, G. L. Lukacs, et al. Molecular Proximity of Cystic Fibrosis Transmembrane Conductance Regulator and Epithelial Sodium Channel Assessed by Fluorescence Resonance Energy Transfer J. Biol. Chem., December 14, 2007; 282(50): 36481 - 36488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Granio, C. Norez, K. J. D. Ashbourne Excoffon, P. H. Karp, M. Lusky, F. Becq, P. Boulanger, J. Zabner, and S.-S. Hong Cellular Localization and Activity of Ad-Delivered GFP-CFTR in Airway Epithelial and Tracheal Cells Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 631 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Panama, M. McLerie, and A. N. Lopatin Heterogeneity of IK1 in the mouse heart Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3558 - H3567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Swiatecka-Urban, L. Talebian, E. Kanno, S. Moreau-Marquis, B. Coutermarsh, K. Hansen, K. H. Karlson, R. Barnaby, R. E. Cheney, G. M. Langford, et al. Myosin Vb Is Required for Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator in Rab11a-specific Apical Recycling Endosomes in Polarized Human Airway Epithelial Cells J. Biol. Chem., August 10, 2007; 282(32): 23725 - 23736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. MacEachran, S. Ye, J. M. Bomberger, D. A. Hogan, A. Swiatecka-Urban, B. A. Stanton, and G. A. O'Toole The Pseudomonas aeruginosa Secreted Protein PA2934 Decreases Apical Membrane Expression of the Cystic Fibrosis Transmembrane Conductance Regulator Infect. Immun., August 1, 2007; 75(8): 3902 - 3912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Swiatecka-Urban, S. Moreau-Marquis, D. P. MacEachran, J. P. Connolly, C. R. Stanton, J. R. Su, R. Barnaby, G. A. O'Toole, and B. A. Stanton Pseudomonas aeruginosa inhibits endocytic recycling of CFTR in polarized human airway epithelial cells Am J Physiol Cell Physiol, March 1, 2006; 290(3): C862 - C872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Panama and A. N. Lopatin Differential polyamine sensitivity in inwardly rectifying Kir2 potassium channels J. Physiol., March 1, 2006; 571(2): 287 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guerra, T. Fanelli, M. Favia, S. M. Riccardi, G. Busco, R. A. Cardone, S. Carrabino, E. J. Weinman, S. J. Reshkin, M. Conese, et al. Na+/H+ Exchanger Regulatory Factor Isoform 1 Overexpression Modulates Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Expression and Activity in Human Airway 16HBE14o- Cells and Rescues {Delta}F508 CFTR Functional Expression in Cystic Fibrosis Cells J. Biol. Chem., December 9, 2005; 280(49): 40925 - 40933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Swiatecka-Urban, A. Brown, S. Moreau-Marquis, J. Renuka, B. Coutermarsh, R. Barnaby, K. H. Karlson, T. R. Flotte, M. Fukuda, G. M. Langford, et al. The Short Apical Membrane Half-life of Rescued {Delta}F508-Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Results from Accelerated Endocytosis of {Delta}F508-CFTR in Polarized Human Airway Epithelial Cells J. Biol. Chem., November 4, 2005; 280(44): 36762 - 36772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Regan-Klapisz, I. Sorokina, J. Voortman, P. de Keizer, R. C. Roovers, P. Verheesen, S. Urbe, L. Fallon, E. A. Fon, A. Verkleij, et al. Ubiquilin recruits Eps15 into ubiquitin-rich cytoplasmic aggregates via a UIM-UBL interaction J. Cell Sci., October 1, 2005; 118(19): 4437 - 4450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. O'Sullivan, M. D. Linden, A. L. Frelinger III, M. R. Barnard, M. Spencer-Manzon, J. E. Morris, R. O. Salem, M. Laposata, and A. D. Michelson Platelet activation in cystic fibrosis Blood, June 15, 2005; 105(12): 4635 - 4641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheng, H. Wang, and W. B. Guggino Regulation of Cystic Fibrosis Transmembrane Regulator Trafficking and Protein Expression by a Rho Family Small GTPase TC10 J. Biol. Chem., February 4, 2005; 280(5): 3731 - 3739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Reigada and C. H. Mitchell Release of ATP from retinal pigment epithelial cells involves both CFTR and vesicular transport Am J Physiol Cell Physiol, January 1, 2005; 288(1): C132 - C140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Younger, H.-Y. Ren, L. Chen, C.-Y. Fan, A. Fields, C. Patterson, and D. M. Cyr A foldable CFTR{Delta}F508 biogenic intermediate accumulates upon inhibition of the Hsc70-CHIP E3 ubiquitin ligase J. Cell Biol., December 20, 2004; 167(6): 1075 - 1085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sugita, H. Kongo, and Y. Shiba Molecular Dissection of the Butyrate Action Revealed the Involvement of Mitogen-Activated Protein Kinase in Cystic Fibrosis Transmembrane Conductance Regulator Biogenesis Mol. Pharmacol., November 1, 2004; 66(5): 1248 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Bilan, V. Thoreau, M. Nacfer, R. Derand, C. Norez, A. Cantereau, M. Garcia, F. Becq, and A. Kitzis Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity J. Cell Sci., April 15, 2004; 117(10): 1923 - 1935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheng, H. Wang, and W. B. Guggino Modulation of Mature Cystic Fibrosis Transmembrane Regulator Protein by the PDZ Domain Protein CAL J. Biol. Chem., January 16, 2004; 279(3): 1892 - 1898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Kowalski and G. B. Pier Localization of Cystic Fibrosis Transmembrane Conductance Regulator to Lipid Rafts of Epithelial Cells Is Required for Pseudomonas aeruginosa-Induced Cellular Activation J. Immunol., January 1, 2004; 172(1): 418 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-M. Zhang, X.-T. Wang, H. Yue, S. W. Leung, P. H. Thibodeau, P. J. Thomas, and S. E. Guggino Organic Solutes Rescue the Functional Defect in {Delta}F508 Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., December 19, 2003; 278(51): 51232 - 51242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Brindikova, N. Bourcier, B. Torres, D. Pchejetski, M. Gekle, G. V. Maximov, V. Montminy, P. A. Insel, S. N. Orlov, and P. Isenring Purinergic-induced signaling in C11-MDCK cells inhibits the secretory Na-K-Cl cotransporter Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1445 - C1453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Bertrand and R. A. Frizzell The role of regulated CFTR trafficking in epithelial secretion Am J Physiol Cell Physiol, July 1, 2003; 285(1): C1 - C18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Tucker, K. Varga, Z. Bebok, A. Zsembery, N. A. McCarty, J. F. Collawn, E. M. Schwiebert, and L. M. Schwiebert Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids Am J Physiol Cell Physiol, March 1, 2003; 284(3): C791 - C804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huang, T. Dudez, I. Scerri, M. A. Thomas, B. N. G. Giepmans, S. Suter, and M. Chanson Defective Activation of c-Src in Cystic Fibrosis Airway Epithelial Cells Results in Loss of Tumor Necrosis Factor-alpha -induced Gap Junction Regulation J. Biol. Chem., February 28, 2003; 278(10): 8326 - 8332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Ameen, C. Marino, and P. J. I. Salas cAMP-dependent exocytosis and vesicle traffic regulate CFTR and fluid transport in rat jejunum in vivo Am J Physiol Cell Physiol, February 1, 2003; 284(2): C429 - C438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fu and E. Sztul Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator J. Cell Biol., January 21, 2003; 160(2): 157 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-X. Wang, M. Ikeda, and W. B. Guggino The Cytoplasmic Tail of Large Conductance, Voltage- and Ca2+-activated K+ (MaxiK) Channel Is Necessary for Its Cell Surface Expression J. Biol. Chem., January 17, 2003; 278(4): 2713 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Estell, G. Braunstein, T. Tucker, K. Varga, J. F. Collawn, and L. M. Schwiebert Plasma Membrane CFTR Regulates RANTES Expression via Its C-Terminal PDZ-Interacting Motif Mol. Cell. Biol., January 15, 2003; 23(2): 594 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Peter, K. Varga, Z. Bebok, C. M. McNicholas-Bevensee, L. Schwiebert, E. J. Sorscher, E. M. Schwiebert, and J. F. Collawn Ablation of Internalization Signals in the Carboxyl-terminal Tail of the Cystic Fibrosis Transmembrane Conductance Regulator Enhances Cell Surface Expression J. Biol. Chem., December 13, 2002; 277(51): 49952 - 49957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Lyczak and G. B. Pier Salmonella enterica Serovar Typhi Modulates Cell Surface Expression of Its Receptor, the Cystic Fibrosis Transmembrane Conductance Regulator, on the Intestinal Epithelium Infect. Immun., November 1, 2002; 70(11): 6416 - 6423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Swiatecka-Urban, M. Duhaime, B. Coutermarsh, K. H. Karlson, J. Collawn, M. Milewski, G. R. Cutting, W. B. Guggino, G. Langford, and B. A. Stanton PDZ Domain Interaction Controls the Endocytic Recycling of the Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., October 11, 2002; 277(42): 40099 - 40105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Haggie, B. A. Stanton, and A. S. Verkman Diffusional Mobility of the Cystic Fibrosis Transmembrane Conductance Regulator Mutant, Delta F508-CFTR, in the Endoplasmic Reticulum Measured by Photobleaching of GFP-CFTR Chimeras J. Biol. Chem., May 3, 2002; 277(19): 16419 - 16425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. S. Marshall, E. M. Lynch, and R. R. F. Cozzi Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water J. Exp. Biol., May 1, 2002; 205(9): 1265 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Gelman, E. S. Kannegaard, and R. R. Kopito A Principal Role for the Proteasome in Endoplasmic Reticulum-associated Degradation of Misfolded Intracellular Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., March 29, 2002; 277(14): 11709 - 11714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Yoo, B. D. Moyer, S. Bannykh, H.-M. Yoo, J. R. Riordan, and W. E. Balch Non-conventional Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator through the Early Secretory Pathway J. Biol. Chem., March 22, 2002; 277(13): 11401 - 11409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. S. Marshall, J. A. Howard, R. R. F. Cozzi, and E. M. Lynch NaCl and fluid secretion by the intestine of the teleost Fundulus heteroclitus: involvement of CFTR J. Exp. Biol., March 15, 2002; 205(6): 745 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Chang, A. Di, A. P. Naren, H. C. Palfrey, K. L. Kirk, and D. J. Nelson Mechanisms of CFTR regulation by syntaxin 1A and PKA J. Cell Sci., February 15, 2002; 115(4): 783 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheng, B. D. Moyer, M. Milewski, J. Loffing, M. Ikeda, J. E. Mickle, G. R. Cutting, M. Li, B. A. Stanton, and W. B. Guggino A Golgi-associated PDZ Domain Protein Modulates Cystic Fibrosis Transmembrane Regulator Plasma Membrane Expression J. Biol. Chem., January 25, 2002; 277(5): 3520 - 3529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Loffing-Cueni, J. Loffing, C. Shaw, A. M. Taplin, M. Govindan, C. R. Stanton, and B. A. Stanton Trafficking of GFP-tagged Delta F508-CFTR to the plasma membrane in a polarized epithelial cell line Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1889 - C1897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Z. Rappoport, M. S. Lipkowitz, and R. G. Abramson Localization and topology of a urate transporter/channel, a galectin, in epithelium-derived cells Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1926 - C1939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Rajan, M. E. Illing, N. F. Bence, and R. R. Kopito Specificity in intracellular protein aggregation and inclusion body formation PNAS, October 25, 2001; (2001) 181479798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Blazer-Yost, M. Butterworth, A. D. Hartman, G. E. Parker, C. J. Faletti, W. J. Els, and S. J. Rhodes Characterization and imaging of A6 epithelial cell clones expressing fluorescently labeled ENaC subunits Am J Physiol Cell Physiol, August 1, 2001; 281(2): C624 - C632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Schwahn, N. Paland, S. Techritz, S. Lenzner, and W. Berger Mutations in the X-linked RP2 gene cause intracellular misrouting and loss of the protein Hum. Mol. Genet., May 1, 2001; 10(11): 1177 - 1183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Maitra, C. M. Shaw, B. A. Stanton, and J. W. Hamilton Increased functional cell surface expression of CFTR and {Delta}F508-CFTR by the anthracycline doxorubicin Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1031 - C1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Milewski, J. Mickle, J. Forrest, D. Stafford, B. Moyer, J Cheng, W. Guggino, B. Stanton, and G. Cutting A PDZ-binding motif is essential but not sufficient to localize the C terminus of CFTR to the apical membrane J. Cell Sci., January 2, 2001; 114(4): 719 - 726. [Abstract] [PDF]
|
|
|
|
|
|
M. Howard, X. Jiang, D. B. Stolz, W. G. Hill, J. A. Johnson, S. C. Watkins, R. A. Frizzell, C. M. Bruton, P. D. Robbins, and O. A. Weisz Forskolin-induced apical membrane insertion of virally expressed, epitope-tagged CFTR in polarized MDCK cells Am J Physiol Cell Physiol, August 1, 2000; 279(2): C375 - C382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Gerceker, T. Zaidi, P. Marks, D. E. Golan, and G. B. Pier Impact of Heterogeneity within Cultured Cells on Bacterial Invasion: Analysis of Pseudomonas aeruginosa and Salmonella enterica Serovar Typhi Entry into MDCK cells by Using a Green Fluorescent Protein-Labelled Cystic Fibrosis Transmembrane Conductance Regulator Receptor Infect. Immun., February 1, 2000; 68(2): 861 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tsukita and S. Yonemura Cortical Actin Organization: Lessons from ERM (Ezrin/Radixin/Moesin) Proteins J. Biol. Chem., December 3, 1999; 274(49): 34507 - 34510. [Full Text] [PDF]
|
|
|
|
 |
|
 B. D. Moyer, D. Loffing-Cueni, J. Loffing, D. Reynolds, and B. A. Stanton Butyrate increases apical membrane CFTR but reduces chloride secretion in MDCK cells Am J Physiol Renal Physiol, August 1, 1999; 277(2): F271 - F276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Ferguson and W. F. Flintoff Topological and Functional Analysis of the Human Reduced Folate Carrier by Hemagglutinin Epitope Insertion J. Biol. Chem., June 4, 1999; 274(23): 16269 - 16278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Kachinsky, S. C. Froehner, and S. L. Milgram A PDZ-containing Scaffold Related to the Dystrophin Complex at the Basolateral Membrane of Epithelial Cells J. Cell Biol., April 19, 1999; 145(2): 391 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Johnston, C. L. Ward, and R. R. Kopito Aggresomes: A Cellular Response to Misfolded Proteins J. Cell Biol., December 28, 1998; 143(7): 1883 - 1898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-L. Ji, M. L. Chalfant, B. Jovov, J. P. Lockhart, S. B. Parker, C. M. Fuller, B. A. Stanton, and D. J. Benos The Cytosolic Termini of the beta - and gamma -ENaC Subunits Are Involved in the Functional Interactions between Cystic Fibrosis Transmembrane Conductance Regulator and Epithelial Sodium Channel J. Biol. Chem., September 1, 2000; 275(36): 27947 - 27956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Moyer, M. Duhaime, C. Shaw, J. Denton, D. Reynolds, K. H. Karlson, J. Pfeiffer, S. Wang, J. E. Mickle, M. Milewski, et al. The PDZ-interacting Domain of Cystic Fibrosis Transmembrane Conductance Regulator Is Required for Functional Expression in the Apical Plasma Membrane J. Biol. Chem., August 25, 2000; 275(35): 27069 - 27074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Rajan, M. E. Illing, N. F. Bence, and R. R. Kopito Specificity in intracellular protein aggregation and inclusion body formation PNAS, November 6, 2001; 98(23): 13060 - 13065. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
