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J Biol Chem, Vol. 273, Issue 34, 21825-21833, August 21, 1998

Trypanosoma brucei TBRGG1, a Mitochondrial Oligo(U)-binding Protein That Co-localizes with an in Vitro RNA Editing Activity

Luc Vanhamme, David Perez-Morga, Christian Marchal, Dave Speijer§, Laurence Lambert, Maurice Geuskens, Sylvie Alexandre, Naïma Ismaïli, Ulrich Göringer, Rob Benne§, and Etienne Pays

From the Department of Molecular Biology, Free University of Brussels, 67 rue des Chevaux, B1640 Rhode St. Genèse, Belgium, § Department of Biochemistry, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands, and  Gene Centre Munich, Max-Planck Institute for Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany

We report the characterization of a Trypanosoma brucei 75-kDa protein of the RGG (Arg-Gly-Gly) type, termed TBRGG1. Dicistronic and monocistronic transcripts of the TBRGG1 gene were produced by both alternative splicing and polyadenylation. TBRGG1 was found in two or three forms that differ in their electrophoretic mobility on SDS-polyacrylamide gel electrophoresis gels, one of which was more abundant in the procyclic form of the parasite. TBRGG1 was localized to the mitochondrion and appeared to be more abundant in bloodstream intermediate and stumpy forms in which the mitochondrion reactivates and during the procyclic stage, which possesses a fully functional mitochondrion. This protein was characterized to display oligo(U) binding characteristics and was found to co-localize with an in vitro RNA editing activity in a sedimentation analysis. TBRGG1 most likely corresponds to the 83-kDa oligo(U)-binding protein previously identified by UV cross-linking of guide RNA to mitochondrial lysates (Leegwater, P., Speijer, D., and Benne, R. (1995) Eur. J. Biochem. 227, 780-786).

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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