HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Myslinski, E. Articles by Carbon, P. Search for Related Content PubMed PubMed Citation Articles by Myslinski, E. Articles by Carbon, P. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 34, 21998-22006, August 21, 1998

ZNF76 and ZNF143 Are Two Human Homologs of the Transcriptional Activator Staf

From the UPR 9002 du CNRS "Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance," IBMC, 15, rue René Descartes, 67084 Strasbourg Cedex, France

The transcriptional activator Staf, originally identified in Xenopus laevis, is implicated in the enhanced transcription of small nuclear RNA (snRNA) and snRNA-type genes by RNA polymerases II (Pol II) and III (Pol III). This zinc finger protein also possesses the capacity to stimulate expression from a Pol II mRNA promoter. Here, we report a study on two human proteins, ZNF76 and ZNF143, that are 64 and 84% identical to their Xenopus counterpart, respectively. Northern blot analysis revealed that ZNF76 and ZNF143 mRNAs were expressed in all normal adult tissues examined. By using in vivo and in vitro assays, we have analyzed the DNA binding capacities and transcriptional properties of ZNF76 and ZNF143. The binding affinities of ZNF76 and ZNF143 for Staf divergent responsive elements were determined by gel shift assays, which revealed that the two proteins bound a same DNA motif with similar affinities. Also, polypeptide sequences containing the seven zinc fingers of ZNF76 and ZNF143 could efficiently repress in vivo the activated transcription from an snRNA-type promoter. Transfection experiments in Drosophila cells showed that ZNF76 and ZNF143 can activate transcription from an mRNA promoter through the Staf binding site. Finally, chimeric ZNF76 and ZNF143 proteins, carrying a heterologous DNA binding domain, are able to activate a Pol II mRNA promoter and snRNA Pol II and Pol III promoters in Xenopus oocytes, through the heterologous DNA binding site. Taken together, these findings demonstrate that ZNF76 and ZNF143 are two members of a same family of transactivator proteins. ZNF143 constitutes the human ortholog of the Xenopus Staf, and ZNF76 is a novel DNA binding protein related to Staf and ZNF143.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				B. A. Thompson, V. Tremblay, G. Lin, and D. A. Bochar CHD8 Is an ATP-Dependent Chromatin Remodeling Factor That Regulates {beta}-Catenin Target Genes Mol. Cell. Biol., June 15, 2008; 28(12): 3894 - 3904. [Abstract] [Full Text] [PDF]

				C.-C. Yuan, X. Zhao, L. Florens, S. K. Swanson, M. P. Washburn, and N. Hernandez CHD8 Associates with Human Staf and Contributes to Efficient U6 RNA Polymerase III Transcription Mol. Cell. Biol., December 15, 2007; 27(24): 8729 - 8738. [Abstract] [Full Text] [PDF]

				E. Myslinski, M.-A. Gerard, A. Krol, and P. Carbon Transcription of the human cell cycle regulated BUB1B gene requires hStaf/ZNF143 Nucleic Acids Res., May 11, 2007; 35(10): 3453 - 3464. [Abstract] [Full Text] [PDF]

				I. B. Jeffery, S. F. Madden, P. A. McGettigan, G. Perriere, A. C. Culhane, and D. G. Higgins Integrating transcription factor binding site information with gene expression datasets Bioinformatics, February 1, 2007; 23(3): 298 - 305. [Abstract] [Full Text] [PDF]

				E. Myslinski, M.-A. Gerard, A. Krol, and P. Carbon A Genome Scale Location Analysis of Human Staf/ZNF143-binding Sites Suggests a Widespread Role for Human Staf/ZNF143 in Mammalian Promoters J. Biol. Chem., December 29, 2006; 281(52): 39953 - 39962. [Abstract] [Full Text] [PDF]

				J. R. Prigge and E. E. Schmidt Interaction of Protein Inhibitor of Activated STAT (PIAS) Proteins with the TATA-binding Protein, TBP J. Biol. Chem., May 5, 2006; 281(18): 12260 - 12269. [Abstract] [Full Text] [PDF]

				V. P. Kelly, T. Suzuki, O. Nakajima, T. Arai, Y. Tamai, S. Takahashi, S. Nishimura, and M. Yamamoto The Distal Sequence Element of the Selenocysteine tRNA Gene Is a Tissue-Dependent Enhancer Essential for Mouse Embryogenesis Mol. Cell. Biol., May 1, 2005; 25(9): 3658 - 3669. [Abstract] [Full Text] [PDF]

				G. Zheng and Y.-C. Yang ZNF76, a Novel Transcriptional Repressor Targeting TATA-binding Protein, Is Modulated by Sumoylation J. Biol. Chem., October 8, 2004; 279(41): 42410 - 42421. [Abstract] [Full Text] [PDF]

				C. E. Grossman, Y. Qian, K. Banki, and A. Perl ZNF143 Mediates Basal and Tissue-specific Expression of Human Transaldolase J. Biol. Chem., March 26, 2004; 279(13): 12190 - 12205. [Abstract] [Full Text] [PDF]

				F. Di Leva, M. I. Ferrante, F. Demarchi, A. Caravelli, M. R. Matarazzo, M. Giacca, M. D'Urso, M. D'Esposito, and A. Franze Human Synaptobrevin-like 1 Gene Basal Transcription Is Regulated through the Interaction of Selenocysteine tRNA Gene Transcription Activating Factor-Zinc Finger 143 Factors with Evolutionary Conserved Cis-elements J. Biol. Chem., February 27, 2004; 279(9): 7734 - 7739. [Abstract] [Full Text] [PDF]

				D. Saur, B. Seidler, H. Paehge, V. Schusdziarra, and H.-D. Allescher Complex Regulation of Human Neuronal Nitric-oxide Synthase Exon 1c Gene Transcription. ESSENTIAL ROLE OF Sp AND ZNF FAMILY MEMBERS OF TRANSCRIPTION FACTORS J. Biol. Chem., July 5, 2002; 277(28): 25798 - 25814. [Abstract] [Full Text] [PDF]

				E. Myslinski, J.-C. Ame, A. Krol, and P. Carbon An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene Nucleic Acids Res., June 15, 2001; 29(12): 2502 - 2509. [Abstract] [Full Text] [PDF]

				H. A. Hirsch, L. Gu, and R. W. Henry The Retinoblastoma Tumor Suppressor Protein Targets Distinct General Transcription Factors To Regulate RNA Polymerase III Gene Expression Mol. Cell. Biol., December 15, 2000; 20(24): 9182 - 9191. [Abstract] [Full Text]

				M. Schaub, A. Krol, and P. Carbon Structural organization of Staf-DNA complexes Nucleic Acids Res., May 15, 2000; 28(10): 2114 - 2121. [Abstract] [Full Text] [PDF]

				M. Schaub, E. Myslinski, A. Krol, and P. Carbon Maximization of Selenocysteine tRNA and U6 Small Nuclear RNA Transcriptional Activation Achieved by Flexible Utilization of a Staf Zinc Finger J. Biol. Chem., August 27, 1999; 274(35): 25042 - 25050. [Abstract] [Full Text] [PDF]

				M. Schaub, A. Krol, and P. Carbon Flexible Zinc Finger Requirement for Binding of the Transcriptional Activator Staf to U6 Small Nuclear RNA and tRNASec Promoters J. Biol. Chem., August 20, 1999; 274(34): 24241 - 24249. [Abstract] [Full Text] [PDF]

				H. Kubota, S.-i. Yokota, H. Yanagi, and T. Yura Transcriptional Regulation of the Mouse Cytosolic Chaperonin Subunit Gene Ccta/t-Complex Polypeptide 1 by Selenocysteine tRNA Gene Transcription Activating Factor Family Zinc Finger Proteins J. Biol. Chem., September 8, 2000; 275(37): 28641 - 28648. [Abstract] [Full Text] [PDF]