JBC Ideal method for primary cell transfection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rouault, J.-P.
Right arrow Articles by Corbo, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rouault, J.-P.
Right arrow Articles by Corbo, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 35, 22563-22569, August 28, 1998

Interaction of BTG1 and p53-regulated BTG2 Gene Products with mCaf1, the Murine Homolog of a Component of the Yeast CCR4 Transcriptional Regulatory Complex

Jean-Pierre RouaultDagger , Déborah PrévôtDagger , Cyril BerthetDagger , Anne-Marie BirotDagger , Marc Billaud**, Jean-Pierre MagaudDagger Dagger Dagger , and Laura CorboDagger

From the Dagger  Unité INSERM U453, Centre Léon Bérard, 69373 Lyon Cedex 08, France, the Dagger Dagger  Laboratoire de Cytogénétique Moléculaire, Hôpital Edouard Herriot, 69373 Lyon Cedex 03, France, and the ** Laboratoire de Génétique, UMR 5641 CNRS, Domaine Rockefeller, Université Claude Bernard, Lyon 1, France

Both BTG1 and BTG2 are involved in cell-growth control. BTG2 expression is regulated by p53, and its inactivation in embryonic stem cells leads to the disruption of DNA damage-induced G2/M cell-cycle arrest. In order to investigate the mechanism underlying Btg-mediated functions, we looked for possible functional partners of Btg1 and Btg2. Using yeast two-hybrid screening, protein-binding assays, and transient transfection assays in HeLa cells, we demonstrated the physical in vitro and in vivo interaction of both Btg1 and Btg2 with the mouse protein mCaf1 (i.e. mouse CCR4-associated factor 1).

mCaf1 was identified through its interaction with the CCR4 protein, a component of a general transcription multisubunit complex, which, in yeast, regulates the expression of different genes involved in cell-cycle regulation and progression. These data suggest that Btg proteins, through their association with mCaf1, may participate, either directly or indirectly, in the transcriptional regulation of the genes involved in the control of the cell cycle. Finally, we found that box B, one of two conserved domains which define the Btg family, plays a functional role, namely that it is essential to the Btg-mCaf1 interaction.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
Y. Robin-Lespinasse, S. Sentis, C. Kolytcheff, M.-C. Rostan, L. Corbo, and M. Le Romancer
hCAF1, a new regulator of PRMT1-dependent arginine methylation
J. Cell Sci., February 15, 2007; 120(4): 638 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Kawakubo, E. Brachtel, T. Hayashida, G. Yeo, J. Kish, A. Muzikansky, P. D. Walden, and S. Maheswaran
Loss of B-cell translocation gene-2 in estrogen receptor-positive breast carcinoma is associated with tumor grade and overexpression of cyclin d1 protein.
Cancer Res., July 15, 2006; 66(14): 7075 - 7082.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. E. Thompson, L. B. Lamont, and J. Kimble
Germ-line induction of the Caenorhabditis elegans vulva
PNAS, January 17, 2006; 103(3): 620 - 625.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Sentis, M. Le Romancer, C. Bianchin, M.-C. Rostan, and L. Corbo
Sumoylation of the Estrogen Receptor {alpha} Hinge Region Regulates Its Transcriptional Activity
Mol. Endocrinol., November 1, 2005; 19(11): 2671 - 2684.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Hong, M. S. Ryu, and I. K. Lim
Phosphorylation of Serine 147 of tis21/BTG2/pc3 by p-Erk1/2 Induces Pin-1 Binding in Cytoplasm and Cell Death
J. Biol. Chem., June 3, 2005; 280(22): 21256 - 21263.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
K. Okochi, T. Suzuki, J.-i. Inoue, S. Matsuda, and T. Yamamoto
Interaction of anti-proliferative protein Tob with poly(A)-binding protein and inducible poly(A)-binding protein: implication of Tob in translational control
Genes Cells, February 1, 2005; 10(2): 151 - 163.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Park, Y. J. Lee, H.-J. Lee, T. Seki, K.-H. Hong, J. Park, H. Beppu, I. K. Lim, J.-W. Yoon, E. Li, et al.
B-Cell Translocation Gene 2 (Btg2) Regulates Vertebral Patterning by Modulating Bone Morphogenetic Protein/Smad Signaling
Mol. Cell. Biol., December 1, 2004; 24(23): 10256 - 10262.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Berthet, A.-M. Morera, M.-J. Asensio, M.-A. Chauvin, A.-P. Morel, F. Dijoud, J.-P. Magaud, P. Durand, and J.-P. Rouault
CCR4-Associated Factor CAF1 Is an Essential Factor for Spermatogenesis
Mol. Cell. Biol., July 1, 2004; 24(13): 5808 - 5820.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Canzoniere, S. Farioli-Vecchioli, F. Conti, M. T. Ciotti, A. M. Tata, G. Augusti-Tocco, E. Mattei, M. K. Lakshmana, V. Krizhanovsky, S. A. Reeves, et al.
Dual Control of Neurogenesis by PC3 through Cell Cycle Inhibition and Induction of Math1
J. Neurosci., March 31, 2004; 24(13): 3355 - 3369.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
W. J. Bakker, M. Blazquez-Domingo, A. Kolbus, J. Besooyen, P. Steinlein, H. Beug, P. J. Coffer, B. Lowenberg, M. von Lindern, and T. B. van Dijk
FoxO3a regulates erythroid differentiation and induces BTG1, an activator of protein arginine methyl transferase 1
J. Cell Biol., January 19, 2004; 164(2): 175 - 184.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Lee, S. Cha, M.-S. Lee, G. J. Cho, W. S. Choi, and K. Suk
Role of Antiproliferative B Cell Translocation Gene-1 as an Apoptotic Sensitizer in Activation-Induced Cell Death of Brain Microglia
J. Immunol., December 1, 2003; 171(11): 5802 - 5811.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A.-P. Morel, S. Sentis, C. Bianchin, M. Le Romancer, L. Jonard, M.-C. Rostan, R. Rimokh, and L. Corbo
BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms
J. Cell Sci., July 15, 2003; 116(14): 2929 - 2936.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Yoshida, T. Nakamura, M. Komoda, H. Satoh, T. Suzuki, J. K. Tsuzuku, T. Miyasaka, E. H. Yoshida, H. Umemori, R. K. Kunisaki, et al.
Mice lacking a transcriptional corepressor Tob are predisposed to cancer
Genes & Dev., May 15, 2003; 17(10): 1201 - 1206.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Suzuki, J. K-Tsuzuku, R. Ajima, T. Nakamura, Y. Yoshida, and T. Yamamoto
Phosphorylation of three regulatory serines of Tob by Erk1 and Erk2 is required for Ras-mediated cell proliferation and transformation
Genes & Dev., June 1, 2002; 16(11): 1356 - 1370.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
M. A. Ficazzola, M. Fraiman, J. Gitlin, K. Woo, J. Melamed, M. A. Rubin, and P. D. Walden
Antiproliferative B cell translocation gene 2 protein is down-regulated post-transcriptionally as an early event in prostate carcinogenesis
Carcinogenesis, August 1, 2001; 22(8): 1271 - 1279.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P.-J. Chen, S. Cho, S.-W. Jin, and R. E. Ellis
Specification of Germ Cell Fates by FOG-3 Has Been Conserved During Nematode Evolution
Genetics, August 1, 2001; 158(4): 1513 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-M. Derocq, O. Jbilo, M. Bouaboula, M. Segui, C. Clere, and P. Casellas
Genomic and Functional Changes Induced by the Activation of the Peripheral Cannabinoid Receptor CB2 in the Promyelocytic Cells HL-60. POSSIBLE INVOLVEMENT OF THE CB2 RECEPTOR IN CELL DIFFERENTIATION
J. Biol. Chem., May 19, 2000; 275(21): 15621 - 15628.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Guardavaccaro, G. Corrente, F. Covone, L. Micheli, I. D'Agnano, G. Starace, M. Caruso, and F. Tirone
Arrest of G1-S Progression by the p53-Inducible Gene PC3 Is Rb Dependent and Relies on the Inhibition of Cyclin D1 Transcription
Mol. Cell. Biol., March 1, 2000; 20(5): 1797 - 1815.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
D. Prevot, T. Voeltzel, A.-M. Birot, A.-P. Morel, M.-C. Rostan, J.-P. Magaud, and L. Corbo
The Leukemia-associated Protein Btg1 and the p53-regulated Protein Btg2 Interact with the Homeoprotein Hoxb9 and Enhance Its Transcriptional Activation
J. Biol. Chem., January 7, 2000; 275(1): 147 - 153.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Farjot, A. Sergeant, and I. Mikaelian
A New Nucleoporin-like Protein Interacts with Both HIV-1 Rev Nuclear Export Signal and CRM-1
J. Biol. Chem., June 11, 1999; 274(24): 17309 - 17317.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Iacopetti, M. Michelini, I. Stuckmann, B. Oback, E. Aaku-Saraste, and W. B. Huttner
Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division
PNAS, April 13, 1999; 96(8): 4639 - 4644.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Prevot, A.-P. Morel, T. Voeltzel, M.-C. Rostan, R. Rimokh, J.-P. Magaud, and L. Corbo
Relationships of the Antiproliferative Proteins BTG1 and BTG2 with CAF1, the Human Homolog of a Component of the Yeast CCR4 Transcriptional Complex. INVOLVEMENT IN ESTROGEN RECEPTOR alpha SIGNALING PATHWAY
J. Biol. Chem., March 23, 2001; 276(13): 9640 - 9648.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.