HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Blackman, M. J. Articles by Hackett, F. Search for Related Content PubMed PubMed Citation Articles by Blackman, M. J. Articles by Hackett, F. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 36, 23398-23409, September 4, 1998

A Subtilisin-like Protein in Secretory Organelles of Plasmodium falciparum Merozoites

Michael J. Blackman, Hisashi Fujioka^¶, William H. L. Stafford, Mohammed Sajid, Barbara Clough, Suzanne L. Fleck, Masamichi Aikawa, Muni Grainger, and Fiona Hackett

From the  Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom, the ^¶ Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, and the  Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-11, Japan

In the vertebrate host, the malaria parasite invades and replicates asexually within circulating erythrocytes. Parasite proteolytic enzymes play an essential but poorly understood role in erythrocyte invasion. We have identified a Plasmodium falciparum gene, denoted pfsub-1, encoding a member of the subtilisin-like serine protease family (subtilases). The pfsub-1 gene is expressed in asexual blood stages of P. falciparum, and the primary gene product (PfSUB-1) undergoes post-translational processing during secretory transport in a manner consistent with its being converted to a mature, enzymatically active form, as documented for other subtilases. In the invasive merozoite, the putative mature protease (p47) is concentrated in dense granules, which are secretory organelles located toward the apical end of the merozoite. At some point following merozoite release and completion of erythrocyte invasion, p47 is secreted from the parasite in a truncated, soluble form. The subcellular location and timing of secretion of p47 suggest that it is likely to play a role in erythrocyte invasion. PfSUB-1 is a new potential target for antimalarial drug development.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. D. Brydges, X. W. Zhou, M.-H. Huynh, J. M. Harper, J. Mital, K. D. Z. Adjogble, W. Daubener, G. E. Ward, and V. B. Carruthers Targeted Deletion of MIC5 Enhances Trimming Proteolysis of Toxoplasma Invasion Proteins Eukaryot. Cell, December 1, 2006; 5(12): 2174 - 2183. [Abstract] [Full Text] [PDF]

				E. Montero, L. M. Gonzalez, M. Rodriguez, Y. Oksov, M. J. Blackman, and C. A. Lobo A Conserved Subtilisin Protease Identified in Babesia divergens Merozoites J. Biol. Chem., November 24, 2006; 281(47): 35717 - 35726. [Abstract] [Full Text] [PDF]

				L. Jean, F. Hackett, S. R. Martin, and M. J. Blackman Functional Characterization of the Propeptide of Plasmodium falciparum Subtilisin-like Protease-1 J. Biol. Chem., August 1, 2003; 278(31): 28572 - 28579. [Abstract] [Full Text] [PDF]

				S. A. Howell, I. Well, S. L. Fleck, C. Kettleborough, C. R. Collins, and M. J. Blackman A Single Malaria Merozoite Serine Protease Mediates Shedding of Multiple Surface Proteins by Juxtamembrane Cleavage J. Biol. Chem., June 20, 2003; 278(26): 23890 - 23898. [Abstract] [Full Text] [PDF]

				Y. Wu, X. Wang, X. Liu, and Y. Wang Data-Mining Approaches Reveal Hidden Families of Proteases in the Genome of Malaria Parasite Genome Res., April 1, 2003; 13(4): 601 - 616. [Abstract] [Full Text] [PDF]

				N. R. Bradshaw, N. D. Chilcoat, J. W. Verbsky, and A. P. Turkewitz Proprotein Processing within Secretory Dense Core Granules of Tetrahymena thermophila J. Biol. Chem., January 31, 2003; 278(6): 4087 - 4095. [Abstract] [Full Text] [PDF]

				F. Li, A. Dluzewski, A. M. Coley, A. Thomas, L. Tilley, R. F. Anders, and M. Foley Phage-displayed Peptides Bind to the Malarial Protein Apical Membrane Antigen-1 and Inhibit the Merozoite Invasion of Host Erythrocytes J. Biol. Chem., December 20, 2002; 277(52): 50303 - 50310. [Abstract] [Full Text] [PDF]

				M. Kaviratne, S. M. Khan, W. Jarra, and P. R. Preiser Small Variant STEVOR Antigen Is Uniquely Located within Maurer's Clefts in Plasmodium falciparum-Infected Red Blood Cells Eukaryot. Cell, December 1, 2002; 1(6): 926 - 935. [Abstract] [Full Text] [PDF]

				C. Withers-Martinez, J. W. Saldanha, B. Ely, F. Hackett, T. O'Connor, and M. J. Blackman Expression of Recombinant Plasmodium falciparum Subtilisin-like Protease-1 in Insect Cells. CHARACTERIZATION, COMPARISON WITH THE PARASITE PROTEASE, AND HOMOLOGY MODELING J. Biol. Chem., August 9, 2002; 277(33): 29698 - 29709. [Abstract] [Full Text] [PDF]

				J. M. Fakruddin, S. Biswas, and Y. D. Sharma Metalloprotease Activity in a Small Heat Shock Protein of the Human Malaria Parasite Plasmodium vivax Infect. Immun., March 1, 2000; 68(3): 1202 - 1206. [Abstract] [Full Text] [PDF]

				M. Sajid, C. Withers-Martinez, and M. J. Blackman Maturation and Specificity of Plasmodium falciparum Subtilisin-like Protease-1, a Malaria Merozoite Subtilisin-like Serine Protease J. Biol. Chem., January 7, 2000; 275(1): 631 - 641. [Abstract] [Full Text] [PDF]

				C. Withers-Martinez, E. P. Carpenter, F. Hackett, B. Ely, M. Sajid, M. Grainger, and M. J. Blackman PCR-based gene synthesis as an efficient approach for expression of the A+T-rich malaria genome Protein Eng. Des. Sel., December 1, 1999; 12(12): 1113 - 1120. [Abstract] [Full Text] [PDF]

				Y. A. W. Skeiky, M. J. Lodes, J. A. Guderian, R. Mohamath, T. Bement, M. R. Alderson, and S. G. Reed Cloning, Expression, and Immunological Evaluation of Two Putative Secreted Serine Protease Antigens of Mycobacterium tuberculosis Infect. Immun., August 1, 1999; 67(8): 3998 - 4007. [Abstract] [Full Text] [PDF]

				J.-C. Barale, T. Blisnick, H. Fujioka, P. M. Alzari, M. Aikawa, C. Braun-Breton, and G. Langsley Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase PNAS, May 25, 1999; 96(11): 6445 - 6450. [Abstract] [Full Text] [PDF]

				N. G. Seidah, S. J. Mowla, J. Hamelin, A. M. Mamarbachi, S. Benjannet, B. B. Toure, A. Basak, J. S. Munzer, J. Marcinkiewicz, M. Zhong, et al. Mammalian subtilisin/kexin isozyme SKI-1: A widely expressed proprotein convertase with a unique cleavage specificity and cellular localization PNAS, February 16, 1999; 96(4): 1321 - 1326. [Abstract] [Full Text] [PDF]

				M. Hayashi, H. Yamada, T. Mitamura, T. Horii, A. Yamamoto, and Y. Moriyama Vacuolar H+-ATPase Localized in Plasma Membranes of Malaria Parasite Cells, Plasmodium falciparum, Is Involved in Regional Acidification of Parasitized Erythrocytes J. Biol. Chem., October 27, 2000; 275(44): 34353 - 34358. [Abstract] [Full Text] [PDF]

				S. A. Howell, C. Withers-Martinez, C. H. M. Kocken, A. W. Thomas, and M. J. Blackman Proteolytic Processing and Primary Structure of Plasmodium falciparum Apical Membrane Antigen-1 J. Biol. Chem., August 10, 2001; 276(33): 31311 - 31320. [Abstract] [Full Text] [PDF]

				S. A. Miller, E. M. Binder, M. J. Blackman, V. B. Carruthers, and K. Kim A Conserved Subtilisin-like Protein TgSUB1 in Microneme Organelles of Toxoplasma gondii J. Biol. Chem., November 21, 2001; 276(48): 45341 - 45348. [Abstract] [Full Text] [PDF]