|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 36, 23567-23574, September 4, 1998
From the Department of Medical Nutrition, Karolinska Institute,
Huddinge University Hospital, F60 Novum,
S-141 86 Huddinge, Sweden
Several studies have established that the
prolactin (PRL) gene is expressed not only in lactotrophs and
somatotrophs of the anterior pituitary but, albeit to a lesser extent,
in non-pituitary cells like human thymocytes, decidualized endometrium,
mammary glands during lactation, and some human non-pituitary cell
lines. Despite the requirement in the pituitary for the
pituitary-specific transcription factor Pit-1/GHF-1 for PRL expression,
the expression in non-pituitary cells occurs in the absence of
Pit-1/GHF-1 and can be repressed by glucocorticoids. This prompted us
to investigate the transcription factors in non-pituitary cells which
are involved in controlling expression and glucocorticoid repression of
a previously characterized negative glucocorticoid response element
from the bovine prolactin gene (PRL3 nGRE). Here we have demonstrated
that non-pituitary cells (COS-7 and mouse hepatoma Hepa1c1c7 cells) conferred increased expression via the PRL3 nGRE mainly because of the
binding of the ubiquitously expressed POU-homeodomain-containing octamer transcription factor-1 (Oct-1) to an AT-rich sequence present
in the PRL3 sequence. However, full transcriptional activity required
the binding of a second ubiquitously expressed homeodomain-containing protein, Pbx, previously shown to bind cooperatively with several homeotic selector proteins. The Pbx binding site in the PRL3 nGRE, located just upstream of the Oct-1 binding site, showed a strong sequence similarity with known Pbx binding sites and bound Pbx with an
affinity similar to that of other established Pbx target sequences.
Interestingly, both Oct-1 and Pbx binding to the PRL3 nGRE were found
to be required for glucocorticoid repression. Addition of in
vitro translated glucocorticoid receptor DNA binding domain to
the nuclear extract prevented Oct-1 and Pbx from binding to the PRL
element. The involvement of the homeobox protein Pbx in glucocorticoid
repression via an nGRE identifies a new role for this protein.
Glucocorticoids Repress Transcription from a Negative
Glucocorticoid Response Element Recognized by Two
Homeodomain-containing Proteins, Pbx and Oct-1
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Newton and N. S. Holden Separating Transrepression and Transactivation: A Distressing Divorce for the Glucocorticoid Receptor? Mol. Pharmacol., October 1, 2007; 72(4): 799 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. G. d. Paz, S. Simeonidis, C. Leo, D. W. Rose, and T. Collins Regulation of NF-{kappa}B-dependent Gene Expression by the POU Domain Transcription Factor Oct-1 J. Biol. Chem., March 16, 2007; 282(11): 8424 - 8434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Tang, M. Mazur, and P. L. Mellon The Protein Kinase C Pathway Acts through Multiple Transcription Factors to Repress Gonadotropin-Releasing Hormone Gene Expression in Hypothalamic GT1-7 Neuronal Cells Mol. Endocrinol., November 1, 2005; 19(11): 2769 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rave-Harel, M. L. Givens, S. B. Nelson, H. A. Duong, D. Coss, M. E. Clark, S. B. Hall, M. P. Kamps, and P. L. Mellon TALE Homeodomain Proteins Regulate Gonadotropin-releasing Hormone Gene Expression Independently and via Interactions with Oct-1 J. Biol. Chem., July 16, 2004; 279(29): 30287 - 30297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Alcorn, K. N. Islam, P. P. Young, and C. R. Mendelson Glucocorticoid inhibition of SP-A gene expression in lung type II cells is mediated via the TTF-1-binding element Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L767 - L776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Fu and T. E. Porter Glucocorticoid Induction of Lactotrophs and Prolactin Gene Expression in Chicken Embryonic Pituitary Cells: A Delayed Response Relative to Stimulated Growth Hormone Production Endocrinology, March 1, 2004; 145(3): 1322 - 1330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lethimonier, G. Flouriot, O. Kah, and B. Ducouret The Glucocorticoid Receptor Represses the Positive Autoregulation of the Trout Estrogen Receptor Gene by Preventing the Enhancer Effect of a C/EBP{beta}-Like Protein Endocrinology, August 1, 2002; 143(8): 2961 - 2974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Zabel, W. Wheeler, J. J. Weis, and J. H. Weis Yin Yang 1, Oct1, and NFAT-4 Form Repeating, Cyclosporin-Sensitive Regulatory Modules Within the Murine CD21 Intronic Control Region J. Immunol., April 1, 2002; 168(7): 3341 - 3350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. P. Torra, V. Tsibulsky, F. Delaunay, R. Saladin, V. Laudet, J.-C. Fruchart, V. Kosykh, and B. Staels Circadian and Glucocorticoid Regulation of Rev-erb{alpha} Expression in Liver Endocrinology, October 1, 2000; 141(10): 3799 - 3806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-I. Jang, N. Karaman-Jurukovska, M. I. Morasso, P. M. Steinert, and N. G. Markova Complex Interactions between Epidermal POU Domain and Activator Protein 1 Transcription Factors Regulate the Expression of the Profilaggrin Gene in Normal Human Epidermal Keratinocytes J. Biol. Chem., May 12, 2000; 275(20): 15295 - 15304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Sapolsky, L. M. Romero, and A. U. Munck How Do Glucocorticoids Influence Stress Responses? Integrating Permissive, Suppressive, Stimulatory, and Preparative Actions Endocr. Rev., February 1, 2000; 21(1): 55 - 89. [Abstract] [Full Text]
|
|
|
|
|
|
L. I. McKay and J. A. Cidlowski Molecular Control of Immune/Inflammatory Responses: Interactions Between Nuclear Factor-{kappa}B and Steroid Receptor-Signaling Pathways Endocr. Rev., August 1, 1999; 20(4): 435 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Subramaniam, E. Treuter, and S. Okret Receptor Interacting Protein RIP140 Inhibits Both Positive and Negative Gene Regulation by Glucocorticoids J. Biol. Chem., June 18, 1999; 274(25): 18121 - 18127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-M. Ou, J. M. Storring, N. Kushwaha, and P. R. Albert Heterodimerization of Mineralocorticoid and Glucocorticoid Receptors at a Novel Negative Response Element of the 5-HT1A Receptor Gene J. Biol. Chem., April 20, 2001; 276(17): 14299 - 14307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kakizawa, T. Miyamoto, K. Ichikawa, T. Takeda, S. Suzuki, J.-i. Mori, M. Kumagai, K. Yamashita, and K. Hashizume Silencing Mediator for Retinoid and Thyroid Hormone Receptors Interacts with Octamer Transcription Factor-1 and Acts as a Transcriptional Repressor J. Biol. Chem., March 23, 2001; 276(13): 9720 - 9725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, L. Yin, and F. B. Hillgartner The Homeodomain Proteins PBX and MEIS1 Are Accessory Factors That Enhance Thyroid Hormone Regulation of the Malic Enzyme Gene in Hepatocytes J. Biol. Chem., June 22, 2001; 276(26): 23838 - 23848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. La Celle and R. R. Polakowska Human Homeobox HOXA7 Regulates Keratinocyte Transglutaminase Type 1 and Inhibits Differentiation J. Biol. Chem., August 24, 2001; 276(35): 32844 - 32853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. J. Shah, J. A. Iniguez-Lluhi, A. Romanelli, S. R. Kimball, and L. S. Jefferson The Activated Glucocorticoid Receptor Modulates Presumptive Autoregulation of Ribosomal Protein S6 Protein Kinase, p70 S6K J. Biol. Chem., January 18, 2002; 277(4): 2525 - 2533. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |