JBC Invitrogen Ultrasensitive Cytokine Assays

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, L.-P.
Right arrow Articles by Deber, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, L.-P.
Right arrow Articles by Deber, C. M.

J Biol Chem, Vol. 273, Issue 37, 23645-23648, September 11, 1998

COMMUNICATION
Uncoupling Hydrophobicity and Helicity in Transmembrane Segments
alpha -HELICAL PROPENSITIES OF THE AMINO ACIDS IN NON-POLAR ENVIRONMENTS

Li-Ping Liu and Charles M. Deber

From Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8 and the Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada

Although the chains of amino acids in proteins that span the membrane are demonstrably helical and hydrophobic, little attention has been paid toward addressing the range of helical propensities of individual amino acids in the non-polar environment of membranes. Because it is inappropriate to apply soluble protein-based structure prediction algorithms to membrane proteins, we have used de novo designed peptides (KKAAAXAAAAAXAAWAAXAAAKKKK-amide, where X indicates one of the 20 commonly occurring amino acids) that mimic a protein membrane-spanning domain to determine the alpha -helical proclivity of each residue in the isotropic non-polar environment of n-butanol. Peptide helicities measured by circular dichroism spectroscopy were found to range from theta 222 = -17,000 ° (Pro) to -38,800 ° (Ile) in n-butanol. The relative helicity of each amino acid is shown to be well correlated with its occurrence frequency in natural transmembrane segments, indicating that the helical propensity of individual residues in concert with their hydrophobicity may be a key determinant of the conformations of protein segments in membranes.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Koide, K. Ito, and Y. Akiyama
Substrate Recognition and Binding by RseP, an Escherichia coli Intramembrane Protease
J. Biol. Chem., April 11, 2008; 283(15): 9562 - 9570.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. He, R. Eckert, T. Pharm, M. D. Simanian, C. Hu, D. K. Yarbrough, F. Qi, M. H. Anderson, and W. Shi
Novel Synthetic Antimicrobial Peptides against Streptococcus mutans
Antimicrob. Agents Chemother., April 1, 2007; 51(4): 1351 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Esteban-Martin and J. Salgado
Self-Assembling of Peptide/Membrane Complexes by Atomistic Molecular Dynamics Simulations
Biophys. J., February 1, 2007; 92(3): 903 - 912.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Ding, J. K. Rainey, C. Xu, B. D. Sykes, and L. Fliegel
Structural and Functional Characterization of Transmembrane Segment VII of the Na+/H+ Exchanger Isoform 1
J. Biol. Chem., October 6, 2006; 281(40): 29817 - 29829.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Glukhov, M. Stark, L. L. Burrows, and C. M. Deber
Basis for Selectivity of Cationic Antimicrobial Peptides for Bacterial Versus Mammalian Membranes
J. Biol. Chem., October 7, 2005; 280(40): 33960 - 33967.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Y. Choi, A. W. Partridge, C. Daniels, K. Du, G. L. Lukacs, and C. M. Deber
Destabilization of the Transmembrane Domain Induces Misfolding in a Phenotypic Mutant of Cystic Fibrosis Transmembrane Conductance Regulator
J. Biol. Chem., February 11, 2005; 280(6): 4968 - 4974.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. W. Hofmann, K. Weise, J. Ollesch, P. Agrawal, H. Stalz, W. Stelzer, F. Hulsbergen, H. de Groot, K. Gerwert, J. Reed, et al.
De novo design of conformationally flexible transmembrane peptides driving membrane fusion
PNAS, October 12, 2004; 101(41): 14776 - 14781.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Caballero-Herrera and L. Nilsson
Molecular Dynamics Simulations of the E1/E2 Transmembrane Domain of the Semliki Forest Virus
Biophys. J., December 1, 2003; 85(6): 3646 - 3658.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
W. F. DeGrado, H. Gratkowski, and J. D. Lear
How do helix-helix interactions help determine the folds of membrane proteins? Perspectives from the study of homo-oligomeric helical bundles
Protein Sci., April 1, 2003; 12(4): 647 - 665.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. M. Nappi, J. A. Schaefer, and L. M. Petti
Molecular Examination of the Transmembrane Requirements of the Platelet-derived Growth Factor beta Receptor for a Productive Interaction with the Bovine Papillomavirus E5 Oncoprotein
J. Biol. Chem., November 27, 2002; 277(49): 47149 - 47159.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. J. De Rosa, D. Rayes, G. Spitzmaul, and C. Bouzat
Nicotinic Receptor M3 Transmembrane Domain: Position 8' Contributes to Channel Gating
Mol. Pharmacol., August 1, 2002; 62(2): 406 - 414.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W. Hosia, J. Johansson, and W. J. Griffiths
Hydrogen/Deuterium Exchange and Aggregation of a Polyvaline and a Polyleucine {alpha}-Helix Investigated by Matrix-assisted Laser Desorption Ionization Mass Spectrometry
Mol. Cell. Proteomics, August 1, 2002; 1(8): 592 - 597.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F.-X. Ding, D. Schreiber, N. C. VerBerkmoes, J. M. Becker, and F. Naider
The Chain Length Dependence of Helix Formation of the Second Transmembrane Domain of a G Protein-coupled Receptor of Saccharomyces cerevisiae
J. Biol. Chem., April 19, 2002; 277(17): 14483 - 14492.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
C. M. Deber, C. Wang, L.-P. Liu, A. S. Prior, S. Agrawal, B. L. Muskat, and A. J. Cuticchia
TM Finder: A prediction program for transmembrane protein segments using a combination of hydrophobicity and nonpolar phase helicity scales
Protein Sci., January 1, 2001; 10(1): 212 - 219.
[Abstract] [Full Text]

Home page
 J. Cell Biol.Home page
Y. Kida, M. Sakaguchi, M. Fukuda, K. Mikoshiba, and K. Mihara
Membrane Topogenesis of a Type I Signal-Anchor Protein, Mouse Synaptotagmin II, on the Endoplasmic Reticulum
J. Cell Biol., August 21, 2000; 150(4): 719 - 730.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Rosch, D. Naeher, V. Laird, V. Goder, and M. Spiess
The Topogenic Contribution of Uncharged Amino Acids on Signal Sequence Orientation in the Endoplasmic Reticulum
J. Biol. Chem., May 12, 2000; 275(20): 14916 - 14922.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Langosch, B. Brosig, and R. Pipkorn
Peptide Mimics of the Vesicular Stomatitis Virus G-protein Transmembrane Segment Drive Membrane Fusion in Vitro
J. Biol. Chem., August 17, 2001; 276(34): 32016 - 32021.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.