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J Biol Chem, Vol. 273, Issue 37, 23897-23903, September 11, 1998
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From the Several lines of evidence have recently
underscored the significance of fatty acids or fatty acid-derived
metabolites as signaling molecules in adipocyte differentiation. The
acyl-CoA-binding protein (ACBP), which functions as an intracellular
acyl-CoA pool former and transporter, is induced during adipocyte
differentiation. In this report we describe the effects of expression
of high levels of ACBP antisense RNA on the differentiation of 3T3-L1
cells. Pools of 3T3-L1 cells transfected with vectors expressing ACBP antisense RNA showed significantly less lipid accumulation as compared
with cells transfected with the control vector. When individual clones
were analyzed the degree of differentiation at day 10 was inversely
correlated with the level of ACBP antisense RNA expression at day 0. Furthermore, in the clones with the highest levels of ACBP antisense
expression, the induction of expression of the adipogenic transcription
factors peroxisome proliferator-activated receptor Department of Molecular Biology and the
¶ Institute of Biochemistry, Odense University, Campusvej 55, DK-5230 Odense, Denmark
and
CCAAT/enhancer-binding protein 
as well as several adipocyte-specific genes was significantly delayed and reduced. The
adipogenic potential of antisense-expressing cells was partially restored by transfection with a vector expressing high levels of ACBP.
Taken together, these results are strong evidence that inhibition of
differentiation is causally related to the decreased expression of
ACBP, indicating that ACBP plays an important role during adipocyte
differentiation.
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