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J Biol Chem, Vol. 273, Issue 37, 23938-23945, September 11, 1998

The pad1⁺ Gene Encodes a Subunit of the 26 S Proteasome in Fission Yeast

Mary Penney, Caroline Wilkinson, Mairi Wallace, Jean-Paul Javerzat^§, Katherine Ferrell^¶, Michael Seeger^¶, Wolfgang Dubiel^¶, Stuart McKay, Robin Allshire, and Colin Gordon

From the  MRC Human Genetics Unit Western General Hospital, Edinburgh EH4 2XU Scotland, United Kingdom, ^§ Institut de Biochemie et Genetique Cellulaires, 33077 Bordeaux Cedex, France, and ^¶ Institute of Biochemistry Medical Faculty (Charite), Humboldt-University, 10117 Berlin, Germany

We have isolated a fission yeast mutant, mts5-1, in a screen for mutations that confer both methyl 2-benzimidazolecarbamate resistance (MBC^R) and temperature sensitivity (ts) on Schizosaccharomyces pombe. This screen has previously isolated mutations in the 26 S proteasome subunits Mts2, Mts3, and Mts4. We show that the mutation in the mts5-1 strain occurs in the pad1⁺ gene. pad1⁺ was originally isolated on a multicopy plasmid that was capable of conferring staurosporine resistance on a wild type strain. mts5-1/pad1-1 has a similar phenotype to 26 S proteasome mutants previously isolated in the same screen and we show that Pad1 interacts genetically with two of these subunits, Mts3 and Mts4. In this study we describe the identification of Pad1 as a subunit of the 26 S proteasome in fission yeast.

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Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.

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