| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 37, 23952-23958, September 11, 1998
-Na,K-ATPase
From the Department of Pediatrics, Division of Pediatric
Nephrology, Yale University School of Medicine,
New Haven, Connecticut 06520
Ankyrins are a family of adapter molecules that
mediate linkages between integral membrane and cytoskeletal proteins.
Such interactions are crucial to the polarized distribution of membrane proteins in transporting epithelia. We have cloned and characterized a
novel 190-kDa member of this family from a rat kidney cDNA library, which we term AnkG190 based on the predicted size and
homology with the larger neuronal AnkG isoform.
AnkG190 displays a unique 31-residue amino terminus, a
repeats domain consisting of 24 repetitive 33-residue motifs, a
spectrin binding domain, and a truncated regulatory domain. Probes
derived from the unique amino terminus hybridize to an 8-kilobase
message exclusively in kidney and lung and specifically to the kidney
outer medullary collecting ducts by in situ hybridization.
Transfections of Madin-Darby canine kidney and COS-7 epithelial cell
lines with a full-length AnkG190 construct result in
(a) expression at the lateral plasma membrane, (b) functional assembly with the cytoskeleton, and
(c) interaction with at least one membrane protein, the
Na,K-ATPase. Two independent Na,K-ATPase binding domains on
AnkG190 are demonstrated as follows: one within the distal
12 ankyrin repeats, and a second site within the spectrin binding
domain. Thus, ankyrins may interact with integral membrane proteins in
a pleiotropic manner that may involve complex tertiary structural
determinants.
This article has been cited by other articles:
|
|
 |
|
  K. Kizhatil, J. Q. Davis, L. Davis, J. Hoffman, B. L. M. Hogan, and V. Bennett Ankyrin-G Is a Molecular Partner of E-cadherin in Epithelial Cells and Early Embryos J. Biol. Chem., September 7, 2007; 282(36): 26552 - 26561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Cunha and P. J. Mohler Cardiac ankyrins: Essential components for development and maintenance of excitable membrane domains in heart Cardiovasc Res, July 1, 2006; 71(1): 22 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Khundmiri, E. J. Weinman, D. Steplock, J. Cole, A. Ahmad, P. D. Baumann, M. Barati, M. J. Rane, and E. Lederer Parathyroid Hormone Regulation of Na+,K+-ATPase Requires the PDZ 1 Domain of Sodium Hydrogen Exchanger Regulatory Factor-1 in Opossum Kidney Cells J. Am. Soc. Nephrol., September 1, 2005; 16(9): 2598 - 2607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lopez, S. Metral, D. Eladari, S. Drevensek, P. Gane, R. Chambrey, V. Bennett, J.-P. Cartron, C. Le Van Kim, and Y. Colin The Ammonium Transporter RhBG: REQUIREMENT OF A TYROSINE-BASED SIGNAL AND ANKYRIN-G FOR BASOLATERAL TARGETING AND MEMBRANE ANCHORAGE IN POLARIZED KIDNEY EPITHELIAL CELLS J. Biol. Chem., March 4, 2005; 280(9): 8221 - 8228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Mosavi, T. J. Cammett, D. C. Desrosiers, and Z.-y. Peng The ankyrin repeat as molecular architecture for protein recognition Protein Sci., June 1, 2004; 13(6): 1435 - 1448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kizhatil and V. Bennett Lateral Membrane Biogenesis in Human Bronchial Epithelial Cells Requires 190-kDa Ankyrin-G J. Biol. Chem., April 16, 2004; 279(16): 16706 - 16714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lencesova, A. O'Neill, W. G. Resneck, R. J. Bloch, and M. P. Blaustein Plasma Membrane-Cytoskeleton-Endoplasmic Reticulum Complexes in Neurons and Astrocytes J. Biol. Chem., January 23, 2004; 279(4): 2885 - 2893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Del Rio, A. Imam, M. DeLeon, G. Gomez, J. Mishra, Q. Ma, S. Parikh, and P. Devarajan The Death Domain of Kidney Ankyrin Interacts with Fas and Promotes Fas-Mediated Cell Death in Renal Epithelia J. Am. Soc. Nephrol., January 1, 2004; 15(1): 41 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Woroniecki, J. R. Ferdinand, J. S. Morrow, and P. Devarajan Dissociation of spectrin-ankyrin complex as a basis for loss of Na-K-ATPase polarity after ischemia Am J Physiol Renal Physiol, February 1, 2003; 284(2): F358 - F364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gagelin, B. Constantin, C. Deprette, M.-A. Ludosky, M. Recouvreur, J. Cartaud, C. Cognard, G. Raymond, and E. Kordeli Identification of AnkG107, a Muscle-specific Ankyrin-G Isoform J. Biol. Chem., April 5, 2002; 277(15): 12978 - 12987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bennett and A. J. Baines Spectrin and Ankyrin-Based Pathways: Metazoan Inventions for Integrating Cells Into Tissues Physiol Rev, July 1, 2001; 81(3): 1353 - 1392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Williams, W. Resneck, T Kaysser, J. Ursitti, C. Birkenmeier, J. Barker, and R. Bloch Na,K-ATPase in skeletal muscle: two populations of beta-spectrin control localization in the sarcolemma but not partitioning between the sarcolemma and the transverse tubules J. Cell Sci., January 2, 2001; 114(4): 751 - 762. [Abstract] [PDF]
|
|
|
|
|
|
J. G. J. Hoenderop, P. H. G. M. Willems, and R. J. M. Bindels Toward a comprehensive molecular model of active calcium reabsorption Am J Physiol Renal Physiol, March 1, 2000; 278(3): F352 - F360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. De Matteis and J. Morrow Spectrin tethers and mesh in the biosynthetic pathway J. Cell Sci., January 7, 2000; 113(13): 2331 - 2343. [Abstract] [PDF]
|
|
|
|
 |
|
 A. M. Kachinsky, S. C. Froehner, and S. L. Milgram A PDZ-containing Scaffold Related to the Dystrophin Complex at the Basolateral Membrane of Epithelial Cells J. Cell Biol., April 19, 1999; 145(2): 391 - 402. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
