| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 38, 24543-24549, September 18, 1998
From the Department of Minichromosome maintenance (Mcm) proteins and the
constituents of the origin recognition complex (Orc) are essential
components of the eukaryotic replication initiation apparatus.
Published evidence strongly suggests that the binding of Mcm proteins
to chromatin is contingent upon the prior binding of Orc proteins. Here
we use two different approaches to investigate the presence of the
human ORC2 protein and of Mcm proteins on chromatin of HeLa cells in
various cell cycle phases. First, we mobilized chromatin-bound proteins
by micrococcal nuclease and analyzed the resulting digestion products
by sucrose gradient centrifugations. Under digestion conditions when
Mcm proteins were almost entirely released from chromatin, ORC2 protein
was found to be associated with chromatin fragments containing several
hundred base pairs of DNA. Second, we used an in vivo
cross-linking procedure to covalently link Mcm proteins and ORC2 to DNA
by short exposure of intact HeLa cells to formaldehyde. Specific
immunoprecipitations revealed that cross-linked nucleoprotein fragments
carried either Mcm proteins or ORC2 protein, but not both. Based on the
lengths of the DNA fragments in immunoprecipitates, we estimate that
the distance between chromatin-bound ORC2 protein and chromatin-bound
Mcm proteins must be at least 500-1000 base pairs in HeLa cells.
Human Minichromosome Maintenance Proteins and Human Origin
Recognition Complex 2 Protein on Chromatin
,,,
Biology, Universität Konstanz, D-78457
Konstanz, Federal Republic of Germany and ¶ Wellcome/CRC
Institute, Cambridge, CB2 1QR, United Kingdom
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Q. Ge, D. A. Jackson, and J. J. Blow Dormant origins licensed by excess Mcm2 7 are required for human cells to survive replicative stress Genes & Dev., December 15, 2007; 21(24): 3331 - 3341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Liu, J. J. Bissler, R. R. Sinden, and M. Leffak Unstable Spinocerebellar Ataxia Type 10 (ATTCT){middle dot}(AGAAT) Repeats Are Associated with Aberrant Replication at the ATX10 Locus and Replication Origin-Dependent Expansion at an Ectopic Site in Human Cells Mol. Cell. Biol., November 15, 2007; 27(22): 7828 - 7838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Lebofsky, R. Heilig, M. Sonnleitner, J. Weissenbach, and A. Bensimon DNA Replication Origin Interference Increases the Spacing between Initiation Events in Human Cells Mol. Biol. Cell, December 1, 2006; 17(12): 5337 - 5345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gerhardt, S. Jafar, M.-P. Spindler, E. Ott, and A. Schepers Identification of New Human Origins of DNA Replication by an Origin-Trapping Assay Mol. Cell. Biol., October 15, 2006; 26(20): 7731 - 7746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ghosh, M. Kemp, G. Liu, M. Ritzi, A. Schepers, and M. Leffak Differential Binding of Replication Proteins across the Human c-myc Replicator. Mol. Cell. Biol., July 1, 2006; 26(14): 5270 - 5283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Woodward, T. Gohler, M. G. Luciani, M. Oehlmann, X. Ge, A. Gartner, D. A. Jackson, and J. J. Blow Excess Mcm2-7 license dormant origins of replication that can be used under conditions of replicative stress J. Cell Biol., June 5, 2006; 173(5): 673 - 683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Baltin, S. Leist, F. Odronitz, H.-P. Wollscheid, M. Baack, T. Kapitza, D. Schaarschmidt, and R. Knippers DNA Replication in Protein Extracts from Human Cells Requires ORC and Mcm Proteins J. Biol. Chem., May 5, 2006; 281(18): 12428 - 12435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Teer, Y. J. Machida, H. Labit, O. Novac, O. Hyrien, K. Marheineke, M. Zannis-Hadjopoulos, and A. Dutta Proliferating Human Cells Hypomorphic for Origin Recognition Complex 2 and Pre-replicative Complex Formation Have a Defect in p53 Activation and Cdk2 Kinase Activation J. Biol. Chem., March 10, 2006; 281(10): 6253 - 6260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-E. Kim, S. A. McAvoy, D. I. Smith, and J. Chen Human TopBP1 Ensures Genome Integrity during Normal S Phase Mol. Cell. Biol., December 15, 2005; 25(24): 10907 - 10915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sibani, G. B. Price, and M. Zannis-Hadjopoulos Decreased origin usage and initiation of DNA replication in haploinsufficient HCT116 Ku80+/- cells J. Cell Sci., August 1, 2005; 118(15): 3247 - 3261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Miccoli, I. Frouin, O. Novac, D. Di Paola, F. Harper, M. Zannis-Hadjopoulos, G. Maga, D. S. F. Biard, and J. F. Angulo The Human Stress-Activated Protein kin17 Belongs to the Multiprotein DNA Replication Complex and Associates In Vivo with Mammalian Replication Origins Mol. Cell. Biol., May 1, 2005; 25(9): 3814 - 3830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Volkening and I. Hoffmann Involvement of Human MCM8 in Prereplication Complex Assembly by Recruiting hcdc6 to Chromatin Mol. Cell. Biol., February 15, 2005; 25(4): 1560 - 1568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Shechter, C. Y. Ying, and J. Gautier DNA Unwinding Is an MCM Complex-dependent and ATP Hydrolysis-dependent Process J. Biol. Chem., October 29, 2004; 279(44): 45586 - 45593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kulartz and R. Knippers The Replicative Regulator Protein Geminin on Chromatin in the HeLa Cell Cycle J. Biol. Chem., October 1, 2004; 279(40): 41686 - 41694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ekholm-Reed, J. Mendez, D. Tedesco, A. Zetterberg, B. Stillman, and S. I. Reed Deregulation of cyclin E in human cells interferes with prereplication complex assembly J. Cell Biol., June 21, 2004; 165(6): 789 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Forsburg Eukaryotic MCM Proteins: Beyond Replication Initiation Microbiol. Mol. Biol. Rev., March 1, 2004; 68(1): 109 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ritzi, K. Tillack, J. Gerhardt, E. Ott, S. Humme, E. Kremmer, W. Hammerschmidt, and A. Schepers Complex protein-DNA dynamics at the latent origin of DNA replication of Epstein-Barr virus J. Cell Sci., October 1, 2003; 116(19): 3971 - 3984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gregan, K. Lindner, L. Brimage, R. Franklin, M. Namdar, E. A. Hart, S. J. Aves, and S. E. Kearsey Fission Yeast Cdc23/Mcm10 Functions after Pre-replicative Complex Formation To Promote Cdc45 Chromatin Binding Mol. Biol. Cell, September 1, 2003; 14(9): 3876 - 3887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S.F. Biard, L. Miccoli, E. Despras, F. Harper, E. Pichard, C. Creminon, and J. F. Angulo Participation of kin17 Protein in Replication Factories and in Other DNA Transactions Mediated by High Molecular Weight Nuclear Complexes Mol. Cancer Res., May 1, 2003; 1(7): 519 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Schaarschmidt, E.-M. Ladenburger, C. Keller, and R. Knippers Human Mcm proteins at a replication origin during the G1 to S phase transition Nucleic Acids Res., October 1, 2002; 30(19): 4176 - 4185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Edwards, A. V. Tutter, C. Cvetic, C. H. Gilbert, T. A. Prokhorova, and J. C. Walter MCM2-7 Complexes Bind Chromatin in a Distributed Pattern Surrounding the Origin Recognition Complex in Xenopus Egg Extracts J. Biol. Chem., August 30, 2002; 277(36): 33049 - 33057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Keller, E.-M. Ladenburger, M. Kremer, and R. Knippers The Origin Recognition Complex Marks a Replication Origin in the Human TOP1 Gene Promoter J. Biol. Chem., August 23, 2002; 277(35): 31430 - 31440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Novac, D. Alvarez, C. E. Pearson, G. B. Price, and M. Zannis-Hadjopoulos The Human Cruciform-binding Protein, CBP, Is Involved in DNA Replication and Associates in Vivo with Mammalian Replication Origins J. Biol. Chem., March 22, 2002; 277(13): 11174 - 11183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-M. Ladenburger, C. Keller, and R. Knippers Identification of a Binding Region for Human Origin Recognition Complex Proteins 1 and 2 That Coincides with an Origin of DNA Replication Mol. Cell. Biol., February 15, 2002; 22(4): 1036 - 1048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Li and M. L. DePamphilis Mammalian Orc1 Protein Is Selectively Released from Chromatin and Ubiquitinated during the S-to-M Transition in the Cell Division Cycle Mol. Cell. Biol., January 1, 2002; 22(1): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Crevel, A. Ivetic, K. Ohno, M. Yamaguchi, and S. Cotterill Nearest neighbour analysis of MCM protein complexes in Drosophila melanogaster Nucleic Acids Res., December 1, 2001; 29(23): 4834 - 4842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Novac, D. Matheos, F. D. Araujo, G. B. Price, and M. Zannis-Hadjopoulos In Vivo Association of Ku with Mammalian Origins of DNA Replication Mol. Biol. Cell, November 1, 2001; 12(11): 3386 - 3401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bielinsky and S. Gerbi Where it all starts: eukaryotic origins of DNA replication J. Cell Sci., January 2, 2001; 114(4): 643 - 651. [Abstract] [PDF]
|
|
|
|
|
|
G Crevel, H Huikeshoven, and S Cotterill Df31 is a novel nuclear protein involved in chromatin structure in Drosophila melanogaster J. Cell Sci., January 1, 2001; 114(1): 37 - 47. [Abstract] [PDF]
|
|
|
|
|
|
J. Méndez and B. Stillman Chromatin Association of Human Origin Recognition Complex, Cdc6, and Minichromosome Maintenance Proteins during the Cell Cycle: Assembly of Prereplication Complexes in Late Mitosis Mol. Cell. Biol., November 15, 2000; 20(22): 8602 - 8612. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Tatsumi, T. Tsurimoto, K. Shirahige, H. Yoshikawa, and C. Obuse Association of Human Origin Recognition Complex 1 with Chromatin DNA and Nuclease-resistant Nuclear Structures J. Biol. Chem., February 25, 2000; 275(8): 5904 - 5910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ogawa, T. Takahashi, and H. Masukata Association of Fission Yeast Orp1 and Mcm6 Proteins with Chromosomal Replication Origins Mol. Cell. Biol., October 1, 1999; 19(10): 7228 - 7236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Treuner, M. Findeisen, U. Strausfeld, and R. Knippers Phosphorylation of Replication Protein A Middle Subunit (RPA32) Leads to a Disassembly of the RPA Heterotrimer J. Biol. Chem., May 28, 1999; 274(22): 15556 - 15561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z Lygerou and P Nurse The fission yeast origin recognition complex is constitutively associated with chromatin and is differentially modified through the cell cycle J. Cell Sci., January 11, 1999; 112(21): 3703 - 3712. [Abstract] [PDF]
|
|
|
|
|
|
S. Kreitz, M. Ritzi, M. Baack, and R. Knippers The Human Origin Recognition Complex Protein 1 Dissociates from Chromatin during S Phase in HeLa Cells J. Biol. Chem., February 23, 2001; 276(9): 6337 - 6342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kappes, K. Burger, M. Baack, F. O. Fackelmayer, and C. Gruss Subcellular Localization of the Human Proto-oncogene Protein DEK J. Biol. Chem., July 6, 2001; 276(28): 26317 - 26323. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
