JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshima, T.
Right arrow Articles by Yanagi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshima, T.
Right arrow Articles by Yanagi, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 39, 25466-25471, September 25, 1998

Heat Shock Factor 1 Mediates Hemin-induced hsp70 Gene Transcription in K562 Erythroleukemia Cells

Tadahiko Yoshima, Takashi Yura, and Hideki Yanagi

From HSP Research Institute, Kyoto Research Park, Shimogyo-ku, Kyoto 600-8813, Japan

Transcriptional induction of the hsp70 gene is mediated by heat shock factor 1 (HSF1) rapidly activated upon heat and other stresses. HSF2 has been thought to be responsible for accumulation of HSP70 during hemin-induced differentiation of human K562 erythroleukemia cells because of accompanying acquisition of HSF2 DNA binding activity. However, there has not been any direct evidence for such a functional role of HSF2. The purpose of this study is to clarify the roles of HSF1 and HSF2 in HSP70 induction in hemin-treated K562 cells. We show here that a chimeric polypeptide of HSF2 and GAL4 DNA binding domain (GAL4-BD-HSF2) was unable to induce a GAL4 binding site-containing luciferase reporter gene in response to hemin and that exogenously overproduced HSF2 also failed to increase expression of a heat shock element-containing reporter. On the contrary, expression of a GAL4-BD-HSF1 chimeric protein responded to hemin treatment as well as to heat shock, and transiently overexpressed HSF1 caused hemin-responsive induction of the reporter gene in a dose-dependent manner. These results indicate that HSF1, rather than HSF2, primarily mediates the hemin-induced transcription of the hsp70 gene.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. Ostling, J. K. Bjork, P. Roos-Mattjus, V. Mezger, and L. Sistonen
Heat Shock Factor 2 (HSF2) Contributes to Inducible Expression of hsp Genes through Interplay with HSF1
J. Biol. Chem., March 9, 2007; 282(10): 7077 - 7086.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Chang, P. Ostling, M. Akerfelt, D. Trouillet, M. Rallu, Y. Gitton, R. El Fatimy, V. Fardeau, S. Le Crom, M. Morange, et al.
Role of heat-shock factor 2 in cerebral cortex formation and as a regulatorof p35 expression.
Genes & Dev., April 1, 2006; 20(7): 836 - 847.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. He, F. Soncin, N. Grammatikakis, Y. Li, A. Siganou, J. Gong, S. A. Brown, R. E. Kingston, and S. K. Calderwood
Elevated Expression of Heat Shock Factor (HSF) 2A Stimulates HSF1-induced Transcription during Stress
J. Biol. Chem., September 12, 2003; 278(37): 35465 - 35475.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. R. McMillan, E. Christians, M. Forster, X. Xiao, P. Connell, J.-C. Plumier, X. Zuo, J. Richardson, S. Morgan, and I. J. Benjamin
Heat Shock Transcription Factor 2 Is Not Essential for Embryonic Development, Fertility, or Adult Cognitive and Psychomotor Function in Mice
Mol. Cell. Biol., November 15, 2002; 22(22): 8005 - 8014.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Mathew, S. K. Mathur, C. Jolly, S. G. Fox, S. Kim, and R. I. Morimoto
Stress-Specific Activation and Repression of Heat Shock Factors 1 and 2
Mol. Cell. Biol., November 1, 2001; 21(21): 7163 - 7171.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
Z. Fishelson, I. Hochman, L. E. Greene, and E. Eisenberg
Contribution of heat shock proteins to cell protection from complement-mediated lysis
Int. Immunol., August 1, 2001; 13(8): 983 - 991.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Pirkkala, T.-P. Alastalo, X. Zuo, I. J. Benjamin, and L. Sistonen
Disruption of Heat Shock Factor 1 Reveals an Essential Role in the Ubiquitin Proteolytic Pathway
Mol. Cell. Biol., April 15, 2000; 20(8): 2670 - 2675.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
I. R. de Mena, M. A. Fernandez-Moreno, B. Bornstein, L. S. Kaguni, and R. Garesse
Structure and Regulated Expression of the delta -Aminolevulinate Synthase Gene from Drosophila melanogaster
J. Biol. Chem., December 24, 1999; 274(52): 37321 - 37328.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Somasundaram and S. P. Bhat
Canonical Heat Shock Element in the alpha B-crystallin Gene Shows Tissue-specific and Developmentally Controlled Interactions with Heat Shock Factor
J. Biol. Chem., May 26, 2000; 275(22): 17154 - 17159.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.