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Vol. 273, Issue 4, 1911-1916, January 23, 1998

Molecular Cloning of a New Secreted Sulfated Mucin-like Protein With a C-type Lectin Domain That Is Expressed in Lymphoblastic Cells

From the Laboratoire de Virologie, Faculté de Médecine, 06107 Nice Cedex 2, France

We have previously demonstrated hyposialylation of the two major CD45 and leukosialin (CD43) molecules at the surface of latently human immunodeficiency virus type 1-infected CEM T cells (CEM_LAI/NP), (Lefebvre, J. C., Giordanengo, V., Doglio, A., Cagnon, L., Breittmayer, J. P., Peyron, J. F., and Lesimple, J. (1994) Virology 199, 265-274; Lefebvre, J. C., Giordanengo, V., Limouse, M., Doglio, A., Cucchiarini, M., Monpoux, F., Mariani, R., and Peyron, J. F. (1994) J. Exp. Med. 180, 1609-1617). Searching to clarify mechanism(s) of hyposialylation, we observed two sulfated secreted glycoproteins (molecular mass ~47 and ~40 kDa) (P47 and P40), which were differentially sulfated and/or differentially secreted in the culture supernatants of CEM_LAI/NP cells when compared with parental CEM cells. A hybridoma clone (7H1) resulting from the fusion between CEM_LAI/NP and human embryonic fibroblasts MRC5 cells produced very large amounts of P47 that was purified using Jacalin lectin (specific for O-glycans) and microsequenced. Cloning of P47 was achieved using a CEM_LAI/NP cDNA library screened with a degenerate oligonucleotide probe based on its NH₂-terminal amino acid sequence. A single open reading frame encoding a protein of 323 amino acids was deduced from the longest isolated recombinant (1.4 kilobase). P47 is a secreted sulfated protein. It carries an NH₂-terminal RGD (Arg-Gly-Asp) triplet, a striking -helical leucine zipper composed of six heptads, and a C-terminal C-type lectin domain. The NH₂-terminal portion is rich in glutamic acids with a predicted pI of 3.9. In addition, a hinge region with numerous condensed potential sites for O-glycan side chains, which are also the most likely sulfation sites, is located between the RGD and leucine zipper domains. Transcripts were detected in lymphoid tissues (notably bone marrow) and abundantly in T and B lymphoblastoid but very faintly in monocytoid cell lines.

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