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Vol. 273, Issue 4, 2452-2457, January 23, 1998

Role of the Core DNA Polymerase III Subunits at the Replication Fork
IS THE ONLY SUBUNIT REQUIRED FOR PROCESSIVE REPLICATION

Kenneth J. Marians, Hiroshi Hiasa, Deok Ryong Kim^§, and Charles S. McHenry^§

From the  Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021 and the ^§ Department of Biochemistry, Biophysics, and Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262

The DNA polymerase III holoenzyme is composed of 10 subunits. The core of the polymerase contains the catalytic polymerase subunit, , the proofreading 3  5 exonuclease, , and a subunit of unknown function, . The availability of the holoenzyme subunits in purified form has allowed us to investigate their roles at the replication fork. We show here that of the three subunits in the core polymerase, only  is required to form processive replication forks that move at high rates and that exhibit coupled leading- and lagging-strand synthesis in vitro. Taken together with previous data this suggests that the primary determinant of replication fork processivity is the interaction between another holoenzyme subunit, , and the replication fork helicase, DnaB.

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